Sequencing and Bioinformatics Analysis of Kappa-Casein Exon 4 Gene in Iranian Bacterianus and Dromedaries Camels

محورهای موضوعی : Camel

م. طهمورث‪پور ¹ , م.ه. سخاوتی ² , ا.ع. کهبیری ³ , ع. محمدهاشمی ⁴

1 - Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran
2 - Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran
3 - Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran
4 - Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran

تاریخ دریافت : 1393/04/15 تاریخ پذیرش : 1393/08/24 تاریخ انتشار : 1394/12/11

کلید واژه: bioinformatics analysis, &lt, i&gt, Camelus bactrianus&lt, /i&gt, , <i>Camelus dromedaries</i>, κ-casein gene,

چکیده مقاله :

Kappa-casein, as a major protein component in mammalian milk, plays an essential role in formation and stabilization milk micelles and preventing them from aggregating and therefore, helping to keep calcium phosphate in solution and transfer of calcium and phosphors from animal milk to consumers. Therefore, the objective of the current study was to investigate genetic and phylogenetic analysis of exon 4 of k-casein (CSN3) gene in Iranian camels using 10 blood samples from Dromedary camels and 5 blood samples from Bacterian camels. After DNA extraction, partial DNA fragment (713 bp) of the k-casein gene was amplified and sequenced using specific primers by PCR reaction. Obtained sequences were edited and subsequently were compared with other k-casein gene sequences which were submitted in NCBI data base. The results showed that there were no significant variations among analyzed sequences. Furthermore, the Neighbor-Joining phylogenetic analysis showed that according to k-casein gene sequence, Iranian Dromedary and Bacterian camels have the lowest genetic distance with Lama species.

چکیده انگلیسی:

کاپا-کازئین، به عنوان عمده‪ترین پروتئین موجود در شیر، نقش مهمی در شکل‪گیری میسل‪های موجود در شیر و کمک به افزایش حلالیت کلیسم و فسفر را ایفا می‪کند. از اینرو، هدف و رویکرد این مطالعه بررسی ژنتیکی و فیلوژنتیکی ناحیه 4 اگژون ژن کاپا-کازئین (CSN3) در شتر‪های ایران (10 نمونه خون از شتر تک کوهانه و 10 نمونه از شتر دوکوهانه) بود. بعد از استخراج DNA، قسمتی از ناحیه DNA (713 جفت باز) از ژن کاپا کازئین به وسیله پرایمر‪های اختصاصی تکثیر و تعیین توالی و در مرحله بعد با توالی‪های دیگر ژن کاپا-کازئین که توالیشان در بانک اطلاعاتی NCBI موجود بود مقایسه گردید. نتایج نشان داد تفاوت معنی‪داری بین توالی‪های مورد مقایسه مشاهده نشد. همچنین، نتایج آنالیز فیلوژنتیکی نشان داد بر اساس توالی ژن کاپا-کازئین، شتر‪های تک و دو کوهانه ایرانی کمترین تفاوت را باتوالی همین ژن با گونه لاما داشتند.

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