Sequencing and Bioinformatics Analysis of Kappa-Casein Exon 4 Gene in Iranian Bacterianus and Dromedaries Camels
الموضوعات :
م. طهمورثپور
1
(Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran)
م.ه. سخاوتی
2
(Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran)
ا.ع. کهبیری
3
(Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran)
ع. محمدهاشمی
4
(Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran)
الکلمات المفتاحية: bioinformatics analysis, <, i>, Camelus bactrianus<, /i>, , <i>Camelus dromedaries</i>, κ-casein gene,
ملخص المقالة :
Kappa-casein, as a major protein component in mammalian milk, plays an essential role in formation and stabilization milk micelles and preventing them from aggregating and therefore, helping to keep calcium phosphate in solution and transfer of calcium and phosphors from animal milk to consumers. Therefore, the objective of the current study was to investigate genetic and phylogenetic analysis of exon 4 of k-casein (CSN3) gene in Iranian camels using 10 blood samples from Dromedary camels and 5 blood samples from Bacterian camels. After DNA extraction, partial DNA fragment (713 bp) of the k-casein gene was amplified and sequenced using specific primers by PCR reaction. Obtained sequences were edited and subsequently were compared with other k-casein gene sequences which were submitted in NCBI data base. The results showed that there were no significant variations among analyzed sequences. Furthermore, the Neighbor-Joining phylogenetic analysis showed that according to k-casein gene sequence, Iranian Dromedary and Bacterian camels have the lowest genetic distance with Lama species.
Ansari-Renani H., Salehi M., Ebadi Z. and Moradi S. (2010). Identification of hair follicle characteristics and activity of one and two humped camels. Small Rumin. Res. 90, 64-70.
Cui P., Ji R., Ding F., Qi D., Gao H. and Meng H. (2007). A complete mitochondrial genome sequence of the wild two-humped camel (Camelus bactrianus ferus): an evolutionary history of camelidae. BMC Genomics. 8, 241-245.
Giambra I.J., Zubeir E.l. and Erhardt G. (2013). Biochemical and molecular characterization of polymorphisms of αs1-casein in Sudanese camel (Camelus dromedarius) milk. Int. Dairy J. 28, 88-93.
Ikonen T., Ojala M. and Syväoja E.L. (2008). Effects of composite casein and beta-lactoglobulin genotypes on renneting properties and composition of bovine milk by assuming an animal model. J. Dairy Sci. 71, 188-195.
Lutfuaalah G., Amjad A., Amin F. and Ahmad A. (2011). Homology modeling of bovine kappa-casein. J. Chem. Soc. Pakistan.33, 433-438.
Medrano J.F. and Cordova E.A. (1990). Genotyping of bovine kappa-casein loci following DNA sequence amplification. Biotechnology. 8, 144-146.
Nagarajan G., Swami S.K., Ghorui S., Pathak K., Singh R. and Patil N. (2012). Cloning and sequence analysis of IL-2, IL-4 and IFN-γ from Indian Dromedary camels (Camelus dromedarius). Res. Vet. Sci. 92, 420-426.
Pauciullo A., Shuiep E., Cosenza G., Ramunno L. and Erhardt G. (2012). Molecular characterization and genetic variability at κ-casein gene (CSN3) in camels. Gene. 15, 22-30.
RachaganiS. andGupta I.D. (2008). Bovine kappa-casein gene polymorphism and its association with milk production traits. Genet. Mol. Biol. 13, 893-897.
Schlee P. and Rottmann O. (1992). Identification of bovine x-casein C using the polymerase chain reaction. J. Anim. Breed. Genet.109, 153-155.
Schwartz H. (1992). Productive performance and productivity of dromedaries (Camelus dromedarius). Anim. Res. Dev. 35,86-98.
Wilson D.E. (2005). Mammal Species of the World: A Taxonomic and Geographic Reference. JohnsHopkinsUniversity Press. US.
Wilson R.T (1984). The Camel. Longman, London,New York.
