Veterinary Clinical Pathology The Quarterly Scientific Journal: 1 (9)

Open Access Article

1 - Evaluation of the effects of rations with different levels of metabolizable energy on performance of laying hens
علی Nobakht H.R Hassanzadeh سامان Mahdavi

 This study was conducted to evaluate the effects of rations with different levels of energy on performance of laying hens. The experiment was conducted with two hundred and fifty six laying hens of commercial Hi-Line W-36 strain in a completely randomized design w More

 This study was conducted to evaluate the effects of rations with different levels of energy on performance of laying hens. The experiment was conducted with two hundred and fifty six laying hens of commercial Hi-Line W-36 strain in a completely randomized design with 4 treatment and 4 replicates (16 laying hens in each replicate). Treatments included: (1) diet with amount of metabolizable energy recommended by NRC 1994 (as control group),   (2) diet with 10% higher level of metabolizable energy than recommended by NRC 1994, (3) diet with 10% lower level of metabolizable energy than recommended by NRC 1994 and (4) diet with 15% lower level of metabolizable energy than recommended by NRC 1994 that were used for 10 weeks (from 41 to 51 weeks of age). The results indicated that the amount of feed intake was significantly different among treatments (p<0.05). The highest amount of feed intake (130.50 g) was observed in treatment four with 15% lower level of metabolizable energy than recommended by NRC 1994. The amount of feed price was also significantly different between treatments (p<0.05). The lowest feed price per kilogram of egg produced (5705 Rials) was observed in treatment four with 15% lower level of metabolizable energy than recommended by NRC 1994. There were no significant differences in egg traits among treatments. It can be concluded that using diets with 15% lower level of metabolizable energy than recommended by NRC 1994 in laying hens, can reduce feed costs without affecting egg traits.     Manuscript profile

Open Access Article

2 - The effects of date consumption on serumic levels of glucose, lipids and lipoproteins in diabetic rats
بهرام Amouoghli Tabrizi علی Hassanpour وحید Kohi احمد Ostovar آرش Alizade

 Diabetes mellitus is an important problem amongst human and animals. In veterinary medicine too, diabetes mellitus occurs in many animals particularly household pets. This study was conducted in order to evaluate the effect of date consumption on serumic levels of More

 Diabetes mellitus is an important problem amongst human and animals. In veterinary medicine too, diabetes mellitus occurs in many animals particularly household pets. This study was conducted in order to evaluate the effect of date consumption on serumic levels of glucose, triglyceride, cholesterol, LDL, VLDL and HDL in rats suffering from experimental diabetes mellitus. Thirty male wistar rats with the age of 8 weeks and mean body weight of 200±20 gr were selected and divided into 5 groups so that there were 6 rats per group. The groups consisted of day one healthy control, final day healthy control, healthy treatment consuming date, diabetic treatment consuming date and diabetic control group. Diabetic treatment and control groups received 100 mg/kg of alloxan subcutaneously to create experimental diabetes. The two healthy control groups also received equal amounts of normal saline solution subcutaneously. The injections were repeated a week later in all groups. After observing the diabetes symptoms including polydipsia, polyuria, glucosuria and hyperglycemia in groups that were given alloxan, feeding of healthy and diabetic treatment groups with equal ratio of date and pellet was initiated. Final day healthy control group and diabetic control group were fed only with pellet. The groups were fed for 10 days following the start of diabetes symptoms. Blood samples were collected from all groups on day 10. Evaluation of the serumic levels of glucose, cholesterol and LDL revealed statistically significant increase in diabetic control group in comparison with the healthy control groups and also in healthy and  diabetic treatment groups consuming date as compared with diabetic control and healthy groups (p<0.05). Evaluation of the serumic levels of triglyceride in diabetic and healthy treatment groups consuming date did not reveal statistically significant difference with the healthy control groups but revealed a significant decrease in comparison with the diabetic control group. Serumic levels of VLDL in diabetic and healthy treatment groups consuming date indicated significant decrease compared with the diabetic control group (p<0.05) but the changes were not significant in comparison with the healthy control groups. There were no statistically significant differences in serumic levels of HDL amoung the groups. It can be concluded from these results that consumption of date as a natural fruit can be effective in preventing symptoms of diabetes mellitus.   Manuscript profile

Open Access Article

3 - Effect of probiotics in poultry diet on microbial hazards of poultry meat
افشین Javadi حمید Mirzaii عیسی Ebrahimi

        Probiotics are products from microbial cells that have useful influence on health and tranquility of humans. According to numerous studies, many valuable properties such as anti-mutagenic, anti-carcinogenic, boosting body immun More

        Probiotics are products from microbial cells that have useful influence on health and tranquility of humans. According to numerous studies, many valuable properties such as anti-mutagenic, anti-carcinogenic, boosting body immunity and resistance against enteric pathogens have been attributed to probiotics. Therefore the aim of this study is to specify the effect of probiotics use in the diet of broilers on microbial hazards of poultry meat. For this purpose, two groups of treatment and control each containing 40 broiler chicks were chosen and edible probiotic were given to the treatment group throughout the whole 55 days of the rearing period under the same conditions and following slaughter 100 grams of skin and breast meat samples were collected from each carcass and transferred to food hygiene laboratory of the veterinary faculty under sterile conditions. Total microbial count,    Staphylococcus aureous, fecal Streptococci, Clostridium perferingens, Coliforms counts and detection of Salmonella and E.coli were performedon the samples according to standard approaches of Iran. The results were analyzed using independent t-Test and Chi-square test. Comparison of the means of total microbial count, coliform, enteric streptococci and meat staphylococcus counts in the control and treatment group using independent t-Test showed a significant reduction (p<0.005). Also, detection of E.coli in the treatment group showed a significant reduction in comparison with the control group (p<0.05) although, Salmonella and Clostridium reductions were not statistically significant. It seems that oral consumption of probiotics is effective in the reduction of enteric pathogenic bacteria of poultry meat. Therefore, if the frequency and level of microbial contamination of food including poultry meat with these micro-organisms is reduced on effective step is undertaken towards reduction of food born diseases and improvement of hygienic quality and shelf life of poultry meat.    Manuscript profile

Open Access Article

4 - Histopathological evaluation of the effect of vitamin E on rat kidney after complete unilateral urethral obstruction
G.h Mousavi داریوش Mohajeri علی Agebati Maleki رامین Kafash Elahi مهرداد Neshat میرهادی Khayat Nouri

Vitamin E is an important antioxidant, which plays a vital role in preventing free radical induced reperfusion injuries. Any disorder in the normal urinary flow is termed obstructive nephropathy which could ultimately lead to hydronephrosis, atrophy and complete impairm More

Vitamin E is an important antioxidant, which plays a vital role in preventing free radical induced reperfusion injuries. Any disorder in the normal urinary flow is termed obstructive nephropathy which could ultimately lead to hydronephrosis, atrophy and complete impairment of renal function. The aim of this study was to determine the effect of vitamin E on renal tissue following complete unilateral urethral obstruction in the rat. In this experimental study, 30 adult male Sprague-Dawley rats were randomly divided into three groups each containing 10 rats. The first group was considered as the control group. Following Unilateral urethral obstruction (UUO), rats in the second group received olive oil intraperitoneally for 15 days starting one day before surgery. Group three rats were given 50 Iu/kg of Vitamin E intraperitoneally for 15 days starting one day before unilateral obstruction (UUO-Vit E). Rats were euthanized at day 14 after surgery for histopathological evaluation and their left kidneys were collected and fixed in formalin and stained with Hematoxylen and Eosin, Trichrome-masson and Periodic Acid Schiff techniques. Histopathological evaluation revealed dilation of the Bowman’s capsule, severe glomerular and tubular atrophy, periglomerular sclerosis, interstitial infiltration of mononuclear cells, severe diffuse tubular degeneration, epithelial thickening of Bowman’s capsule, perivascular edema, interstitial fibrosis, hemorrhage and sub capsular fibrosis in the UUO group. Vitamin E administration in the UUO-Vit E group significantly decreased renal tissue lesions and fibrosis. The result of the present study indicated that urethral obstruction leads to severe renal fibrosis and tissue damage and concurrent administration of Vitamin E decreases tissue lesions and fibrosis resulting from the obstruction.   Manuscript profile

Open Access Article

5 - Study of the prevalence of parasitical skin infections of rainbow trout (250 – 300gr) in commercial fish farms of East Azarbaijan province
حسین Hashemzadeh Sh Notash مسعود Saeedi

Parasitic infestations constitute an important part of aquatic animal diseases. In order to study the prevalence of parasitic skin infections of rainbow trouts in mordad of 1385, 5 breeding farms were selected and 386 rainbow trouts with the body weight of 250-300 gr we More

Parasitic infestations constitute an important part of aquatic animal diseases. In order to study the prevalence of parasitic skin infections of rainbow trouts in mordad of 1385, 5 breeding farms were selected and 386 rainbow trouts with the body weight of 250-300 gr were collected randomly from each farm and examined. Of the 1930 examined specimens of 5 farms in this study, 14 were infected by Ichthyophthirius sp. (0.7%), 890 by Trichodina sp. (46.1%), 41 by Dactylogyrus sp. (2.1%), 111 by Gyrodactylus sp. (508%) and 874 were without any parasitic infections (45.3%). The highest infection rate belonged to Trichodina sp. Manuscript profile

Open Access Article

6 - The effect of phosphate solublizing bacteria as a novel probiotic on some biochemical parameters, carcass characteristics and performance of broiler chicks
مهدی Ghaderi Jouybari M.A Malbobi مهرداد Irani وحید Rezaei Pour مهدی Mohammad Zadeh Nagharchi

In this study, the effect of Pseudomonas Putida and Pantoa Agglomerans which belong to phosphate solublizing bacteria was investigated as a probiotic on serum concentrations of phosphorous and alkaline phosphatase, some carcass characteristics and performance of broiler More

In this study, the effect of Pseudomonas Putida and Pantoa Agglomerans which belong to phosphate solublizing bacteria was investigated as a probiotic on serum concentrations of phosphorous and alkaline phosphatase, some carcass characteristics and performance of broiler chicks. These bacteria, which were isolated with screening soil samples collected from various regions of Iran, were mixed with the diet. The experiment was conducted on a completely randomized design and a 2×4 factorial arrangement. The factors consisted of 4 probiotic levels (1-no probiotic use, 2- probiotic use through out rearing, 3- probiotic use at the start of rearing and during growth period, 4- probiotic use at the end of the rearing period) and 2 phosphorus levels (1-available, 2-total). In this experiment, 8 treatments, 4 replicates with 20 chicks at each replicate and a total of 640 day old chicks of ROSS strain were used. Comparison of means indicated that the probiotic had significantly increased body weight, feed intake and feed conversion ratio (p<0.05). The novel probiotic had increased carcass yield and serum phosphorus (p<0.05). On the other hand, a significant decreased abdominal fat and serum alkaline phosphatase was observed (p>0.05). Also there was no significant effect on breast and thigh weight (p<0.05). The Results of this study indicated that the employed bacteria increase the performance and bioavailability of phosphorous. Manuscript profile

Open Access Article

7 - Application of genomic densitometry for calculating the relative population of Escherichia Coli in the intestine of broiler chicks
A.R Seidavi

     In this study, the densitometry technique for calculating of the relative population of Escherichia coli in various segments of the intestine of broiler chicks was evaluated. Following preparation of the intestinal contents, the process of extraction More

     In this study, the densitometry technique for calculating of the relative population of Escherichia coli in various segments of the intestine of broiler chicks was evaluated. Following preparation of the intestinal contents, the process of extraction and purification of DNA from the contents of duodenum, jejunum, ileum and cecum was undertaken. A specific polymerase chain reaction (PCR) using two pairs of primers was employed to detect Escherichia coli and total bacteria present in the gastrointestinal tract of the chicks. Specific bands of E.coli were obtained using densitometry and Gel Proc Analyzer software based on linear regression with extrapolation. E.coli populations at different ages were also determined in various segments of the gastrointestinal tract of the chicks. The Results of this experiment indicated that 0.000004%, 0.07%, 0.64% and 2.51% of total bacteria present in the duodenum, jejunum, ileum and cecum respectively consisted of E.coli. Also, E.coli constitutes 1.76, 0.01 and 0.80% of the total intestinal bacteria of chicks at 4, 14 and 30 days of age respectively. Furthermore, it was shown that at 4 days of age, 0.30, 2.05 and 3.97% of the total bacteria present in the jejunum, ileum and cecum respectively were from E.coli species and this bacteria was absent in the duodenum. At 14 days of age these figures were 0.000009%, 0.00011% and 0.08% respectively while at 30 days of age 0.00011%, 0.009% and 2.40% of all bacteria in the duodenum, ileum and cecum were E.coli species and this bacteria was absent in the jejunum. In conclusion, the densitometry method based on PCR results can be regarded as a useful tool for densitometry the relative population E.coli in the gastrointestinal tract of poultry. Manuscript profile

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