Veterinary Clinical Pathology The Quarterly Scientific Journal: 1 (1)

Open Access Article

1 - Identification and determination of effective and immunogenic antigens of Ascaridia galli in poultry reared in Tabriz area
Yaghob Gharehdaghi Naser Hoghooghi Rad Parviz Shayan Ali Eslami

In a trial, in Tabriz area, the ability of an experimental vaccine from the larval homogenate of Ascaridia galli was evaluated during the year 1384. A group of 5 white leghorn hens were immunized twice with 100 micrograms larval homogenate of Ascaridia galli diluted in More

In a trial, in Tabriz area, the ability of an experimental vaccine from the larval homogenate of Ascaridia galli was evaluated during the year 1384. A group of 5 white leghorn hens were immunized twice with 100 micrograms larval homogenate of Ascaridia galli diluted in 1ml PBS and emulsified in 1ml Freund’s adjuvant orally. Booster immunization was also administered orally on day 21 The other group (5 white leghorn hens) received 1 ml PBS emulsified in 1ml of the same adjuvant. On day 33 each hen was administered approximately 10,000 Ascaridia galli infective eggs orally. Blood samples were collected from the animals in a 10 day interval from the first immunization until infective challenge and weekly thereafter until the end of study. Sera were tested by ELISA and Western-blotting. Hens were necropsied 8 weeks post challenge with infective eggs for recovery of the nematodes. Vaccinated hens showed a 76 percent reduction in mean EPG, 74 percent reduction in the average number of male worms, 79 percent reduction in the average number of female worms and 77 percent reduction in the mean number of total worms. Significance in mean optical density of sera in ELISA was noticed (p<0.001). Proteins of Ascaridia galli were separated by SDS-PAGE. The vaccinated sera indicated a high reactivity in Western-Blotting. Manuscript profile

Open Access Article

2 - Investigation of clinical and histopathological effects of Rofecoxib after surgical trauma of the gum in rabbit
Ali Rezaie داریوش Mohajeri آرش Khaki افشین Akbari

  In this study, clinical and histopathological effects of Rofecoxib, which is usually used in surgery as an analgesic and anti inflammatory drug is evaluated on the process of healing following surgical trauma. 15 New Zealand white rabbits with the same More

  In this study, clinical and histopathological effects of Rofecoxib, which is usually used in surgery as an analgesic and anti inflammatory drug is evaluated on the process of healing following surgical trauma. 15 New Zealand white rabbits with the same condition were selected and after numbering, divided to three different groups so that there were 5 rabbits per group. Then in each group after anesthesia  with Xylazine and Ketamine a circular surgical trauma was created in the right lateral region of mandible using punch biopsy and after 12 days, clinical and histopathological effects of treatment with low dose (6 mg/kg/day) and high dose (12 mg/kg/day) Rofecoxib was compared with control group that received placebo. It was evident that use of this drug had positive effects on healing which was obvious from clinical findings and comparison of photomicrographs of the healing site in both low dose and high dose treatment groups compared with control group. Also it was determined that use of high dose of Rofecoxib had better effects on healing process compared to low dose of this drug. Manuscript profile

Open Access Article

3 - A serological survey of ovine and caprine brucellosis in slaughterhouses of East Azerbaijan province during 2004
Afshin Javadi قهرمان Akrami Nojadeh M.R Javadi مهدی Ahmad Khanli

Brucellosis is a zoonotic disease which could be transmitted from animals to humans by ingestion of contaminated raw milk, dairy products or contact with meat or raw animal products. Thus, determination of brucella contaminated sheep and goat meat due to the risk of bru More

Brucellosis is a zoonotic disease which could be transmitted from animals to humans by ingestion of contaminated raw milk, dairy products or contact with meat or raw animal products. Thus, determination of brucella contaminated sheep and goat meat due to the risk of brucellosis transmission to human was the purpose of the present study. For this study, blood samples were collected directly during slaughtering from 441 female sheep and 300 female goats with an age of over a year slaughtered at abattoirs of East Azarbaijan province. At first, positive samples were separated by Rose Bengal test and then quantited with Wright and 2-ME tests. All the results were compared with standard veterinary tables and the data were analyzed using the Chi-Square test. According to this research, the prevalence rate of brucellosis among sheep and goats was 4.53% and 5.33% respectively. The prevalence rate of sheep and goat brucellosis in Shabestar city was significantly higher than other cities (P<0.01) but there was no significant difference between sheep and goat brucellosis. Manuscript profile

Open Access Article

4 - Investigation of the occurrence of pregnancy toxemia in the sheep of Sofian area of Tabriz
Ali Hassanpour Gh Moghaddam بهرام Amoughli Tabrizi حمید Mirzaie کریم Hajipour Sofiani

This study was conducted to investigate the occurrence of pregnancy toxemia in the sheep of Sofian area. In this study 134 blood samples were collected randomly from pregnant ewes before and after parturition, and the mean serum concentration of glucose, betahydroxybuty More

This study was conducted to investigate the occurrence of pregnancy toxemia in the sheep of Sofian area. In this study 134 blood samples were collected randomly from pregnant ewes before and after parturition, and the mean serum concentration of glucose, betahydroxybutyric acid (BHBA), urea and calcium were measured. The mean ± SD of BHBA concentration in prepartum and postpartum periods was 0.46± 0.15 and 0.4± 0.11 mmol/l respectively. There was significant difference between these two periods (P<0.05). The mean ± SD of blood glucose concentration before and after parturation was 51.6± 16.04 and 62.18 ± 15.12 mg/dl respectively with a significant difference between the two periods (P<0.05). There was significant negative correlation between BHBA and serum glucose in prepartum and postpartum periods (r=- 0.59 and r=-0.55) (P<0.01). The mean level of BUN in prepartum period was greater than postpartum period but this difference was not significant. The mean ± SD of serum calcium concentration at prepartum and postpartum periods was 12.59 ± 2.72 and 10.06 ± 0.77 mg/dl respectively with a significant difference between these two periods (P<0.01) but there was no correlation between glucose and calcium concentrations in serum. In this study blood samples were collected randomly from ewes at different ages but there was no correlation between disease occurrence and the age of ewes. Thus considering the results this study it is concluded that pregnant ewes are predisposed to subclinical pregnancy toxemia which is economically important both in pregnant ewes and lambs born from them and preventive measures must be employed. Manuscript profile

Open Access Article

5 - A study on some phenotypic characteristics of the Karabakh horse in comparison with the Kurdish and Arabian horses
payam mosapour A.M Aghazade Ali Hassanpour H.A Mahpeikar مجید Ebrahimi Hamed

In order to preserve genetic resources and revive local breeds within the country, some breed specifications of the Karabakh horse, an important local breed in Azarbaijan and Iran was studied. In the present study after obtaining information from horse owners and club m More

In order to preserve genetic resources and revive local breeds within the country, some breed specifications of the Karabakh horse, an important local breed in Azarbaijan and Iran was studied. In the present study after obtaining information from horse owners and club managers of Karabakh horse, the phenotypic characteristics were completed through relevant measurements. 23 phenotype specifications were measured in 42 Karabakh horses, 32 Kurdish horses and 25 Arabian horses which included: face length, face width, ear length, ear width, height at withers, height at knee, height at arm, diameter of the cannon bone, chest heart girth width, back girth, height at croup, height at hock, neck length, back and loin length, croup length, tail length, body length, dorsal hoof height, lateral hoof height, pastern length, ratio of height to body length and weigh of the body. Overall significant differences were observed for most of the studied characteristics between the Karabakh, Kurdish and Arabian horses. In conclusion it could be mentioned that Karabakh horse is distinguished by specific phenotypic features such as low body weight, short height, broad- face, wide chest, long pastern, wide hoof and medium body length which makes it possible for it to be used in sports such as racing and polo and farming activities. Thus preservation of this heritage and valuable genetic resource in its natural farmlands together whit promotion of cultural aspects by relevant authorities is recommended.  Manuscript profile

Open Access Article

6 - Seroepidemiologic survey of Hydatidosis in northwest Iran by Indirect Immunoflurscent Test
Ahmad Nematollahi رسول Jamali Gh Moghaddam

This survey was conducted to study the seroepidemiology of hydatidosis in northwest of Iran. Totally 852 blood samples from rural inhabitants of Sarab, Meshkin shahr, Moghan and Urmia were collected and their sera were separated. For antigen preparation, the livers and More

This survey was conducted to study the seroepidemiology of hydatidosis in northwest of Iran. Totally 852 blood samples from rural inhabitants of Sarab, Meshkin shahr, Moghan and Urmia were collected and their sera were separated. For antigen preparation, the livers and lungs of sheep infected with hydatid cyst and slaughtered at Tabriz abattoir were collected. The protoscolex was separated from the hydatid cyst and injected into the rabbit diaphragm using the sandwich technique. Then sections of the diaphragm were cut using the cryocut apparatus and fixed onto slides. The prepared sections were examined using marked antihuman globulin stained with Evan’s blue by IFA test. Initially sensitivity and specifity of this test was evaluated on sera of 58 hospitalized hydatidosis patients and 70 healthy persons. The sensitivity and specifity of the test were estimated 88% and 98/6% respectively. In this survey the titers over 1:10 were considered positive. Among 852 tested samples, 30(3/52%) were infected. Infection rate in males was higher than females. The highest infection rate was from Meshkin shahr (3.5%) and the age group of 20-40 years was the highest infected category. Manuscript profile

Open Access Article

7 - Evaluation of the effects of Bacillus subtilis and Bacillus Licheni formis promix on blood metabolites and elements and weight gain in rearing calves
Gh Moghaddam Ahmad Nematollahi

Probiotics are beneficial microorganisms which are colonized in the digestive system of livestock and influence some of the blood parameters through special mechanisms. For this purpose, an experiment was conducted to clarify the influence of promix containing living sp More

Probiotics are beneficial microorganisms which are colonized in the digestive system of livestock and influence some of the blood parameters through special mechanisms. For this purpose, an experiment was conducted to clarify the influence of promix containing living spores of the two bacteria Bacillus subtilis and Bacillus licheniformis on body weight gain, blood metabolites and elements of calves. Twenty male Holstein calves were divided into two groups of ten calves and kept at separate bans. The control group were fed with a standard ration and the treatment group were fed with the same ration plus promix (1.6 × 109 cfu/g feed or 500 g/T feed) for 45 days. Calves were weighed in the 1st, 15th, 30th and 45th days and blood samples were collected for measurement of some metabolites and blood elements. The obtained means were compared using the T- test. The results indicated that the average daily weight gain using promix had a non-significant improvement of 4.8% (p>0.05). Also the average daily weight gain showed a non significant improvement in each of the 3 experimental periods (p>0.05). Blood phosphorus and calcium of calves increased significantly influenced by promix with the increase being non significant in the first experimental period and significant in the second and third periods (p<0.01). With the addition of  the probiotic, the amounts of blood glucose, urea and protein increased non significantly compared to the control group with the increase being non significant in the second and third experimental periods and significant in the first period.  Manuscript profile

Open Access Article

8 - Evaluation of serum calcium, phosphorus, alkaline phosphatase, magnesium, total protein and albumin after ovariectomy in the rat
بهرام Amoughli tabrizi داریوش Mohajeri امین Balila Ali Rezaie مهران Mesgari

Osteoporosis is a systemic disease characterized by decrease in bone mass and micro architectural deterioration of bone tissue. In this study, in order to evaluate the serum concentration of calcium, phosphorus, magnesium, alkaline phosphatase, total protein and albumin More

Osteoporosis is a systemic disease characterized by decrease in bone mass and micro architectural deterioration of bone tissue. In this study, in order to evaluate the serum concentration of calcium, phosphorus, magnesium, alkaline phosphatase, total protein and albumin during osteoporosis, 80 female Sprague-Dawley rats with approximate age of 10 weeks were allocated to 10  groups (3 control, 3 sham, 3 treatment groups and one pure control group). The animals in treatment groups were ovariectomized under general anesthesia whereas only the abdominal wall was incised in the sham groups using the same approach of the treatment groups. Treatment groups and shams were maintained for 5, 12 and 21 weeks respectively after ovariectomy alongside related control groups. Blood samples were collected from the retroorbital sinus at the end of each stage for measurement of the blood parameters. Evaluation of the serum levels of calcium, phosphorus and alkaline phosphatase revealed statistically significant differences between the ovariectomized groups and control groups and also pure control group and sham groups. The highest level of calcium and phosphorus was observed 5 weeks after ovariectomy and the lowest level at 12 and 21 weeks after ovariectomy. The levels of alkaline phosphatase had increased at 5, 12 and 21 weeks after ovariectomy. Statistically the mean levels of magnesium, albumin and total protein did not reveal a significant difference between treatment groups, shams and pure control group. Manuscript profile

Open Access Article

9 - Microscopic study of the histogenesis of ependymal cells located in the lateral ventricles of ovine fetal brain
سیدسجاد Hejazi رسول Shahruz محمد Manafi یوسف Angouri

The aim of this study is the determination of the morphogenesis of ependymal tissue cells during the fetal period to beginning of birth in lateral ventricles of sheep`s brain. The study was in the form of descriptive observation conducted on 50 sheep fetuses which were More

The aim of this study is the determination of the morphogenesis of ependymal tissue cells during the fetal period to beginning of birth in lateral ventricles of sheep`s brain. The study was in the form of descriptive observation conducted on 50 sheep fetuses which were collected randomly. The length of the collected fetuses were measured and their age calculated by the formula x=2.1 (Y+17). Following fixation of the fetal brains in 10% buffered formalin they were separated from the skull and sectioned transversely caudal to the optic chiasma in order to obtain samples from the lateral ventricles. The samples were studied histologically after the histotechnique and staining procedures. Microscopically the group of neuroblastic cells surrounding the lateral ventricles consisted of ventricular and subventricular zones with intense mitotic activity in the ventricular zone. The ependymal epithelial tissue changed from pseudostratified to simple cuboidal during development. The thickness and mitotic activity of the neuroblastic mass at different parts of the lateral ventricular wall had a direct relationship with development of other parts of the brain. There was also a direct relationship between the retention of cilia in ependymal cells and the extension of the choroids plexus.          Manuscript profile

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