List of Articles subclinical ketosis

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  1 - Determination of Diagnostic Value of Novavet ketone meter in the diagnosis of cows with subclinical ketosis
  M.R Taheri Jazi maryam karimi dehkordi
  One of the most important metabolic abnormalities in the early lactation period is ketosis. The gold standard test for the diagnosis of ketosis is the measurement of beta-hydroxybutyrate (BHB) in the blood. In this study, the diagnostic value of the electronic ketone me More

  One of the most important metabolic abnormalities in the early lactation period is ketosis. The gold standard test for the diagnosis of ketosis is the measurement of beta-hydroxybutyrate (BHB) in the blood. In this study, the diagnostic value of the electronic ketone meter, Novavet, for diagnosing cows with ketosis was evaluated in comparison with the standard laboratory method. Blood samples were taken from 68 cows in the period of 7-14 days after calving. In the laboratory, serum BHB was measured by spectrophotometry and Randox kit.  At the time of blood sampling, the amount of BHB in whole blood was determined with ketone meter in the same cows. BHB values obtained from the two methods were analyzed by T-test and Pearson correlation statistical models. In order to determine the best cut points of BHB using Novavet machine, which can make the best distinction between healthy and ketosis cows (based on reference test), also to estimate the sensitivity and specificity of Novavet, the ROC analysis is used. The amount of BHB in whole blood in the manual method is significantly higher than the laboratory method. However, there is a significant and good correlation between these two methods. Also, Novavet at the cut point of 1.2 mmol/l has the highest combination of sensitivity and specificity (100 and 41.9%) compared to the standard test. Due to the good and significant correlation between the Novavet test and its high sensitivity, it can be said that this method has an acceptable accuracy for diagnosing hyperketonemic cows. Manuscript profile
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  2 - Evaluation of oxidative stress in cattle with subclinical ketosis and clinical endometritis
  farhad Balari Mahyari maryam karimi dehkordi mohammadreza nazem
  10.30495/jvcp.2020.670410
  Today, the most important factor in the occurrence of metabolic and reproductive diseases in dairy cattle is the disturbance of natural process in body’s cellular-molecular events that occur after increasing of oxidants and the formation of oxidative stress. The a More

  Today, the most important factor in the occurrence of metabolic and reproductive diseases in dairy cattle is the disturbance of natural process in body’s cellular-molecular events that occur after increasing of oxidants and the formation of oxidative stress. The aim of this study was to evaluate the possibility of a significant association between the serum concentrations of malondialdehyde (MDA) and total antioxidant capacity (TAC), as oxidative stress indexes, with the incidence of subclinical ketosis and clinical endometritis in dairy cows. For this purpose, 101 Holstein dairy cows were divided into 4 groups (healthy, subclinical ketosis, clinical endometritis, and both of them subclinical ketosis-clinical endometritis), and serum levels of MDA and TAC were measured in one month before delivery. The lowest serum concentration of TAC and the highest plasma MDA levels were observed in cows with both of diseases, while control group (healthy cows) had the highest serum concentration of TAC and lowest MDA levels (P≤0.05). There was no significant difference in serum concentrations of TAC and MDA between clinical endometritis and subclinical ketosis groups (p>0.05). The results of this study demonstrated a significant correlation between serum concentrations MDA and TAC with the incidence of clinical endometritis and prevalence of ketosis-endometritis in dairy cows (P≤0.05). Conflict of interest: None declared.Keywords: Malondialdehyde, Total Antioxidant Capacity, Clinical Endometritis, Subclinical Ketosis. Manuscript profile
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  3 - The association of some blood metabolites and NF-κB gene expression with subclinical ketosis in Holstein dairy cows during the transition period
  Saeideh Moradi Gholamali moghaddam Samad lotfollahzade Raziallah Jafari Abbas Rafat
  10.30495/jvcp.2022.1937013.1316
  Subclinical ketosis is one of the most important metabolic diseases which irreversible economic effects on the dairy industry. Subclinical ketosis has been associated with increases reactive oxygen species and oxidative stress in cells. Since the nuclear factor kappa-B More

  Subclinical ketosis is one of the most important metabolic diseases which irreversible economic effects on the dairy industry. Subclinical ketosis has been associated with increases reactive oxygen species and oxidative stress in cells. Since the nuclear factor kappa-B is known as a fast cellular response factor to endogenous stress; this study was conducted to evaluate relative gene expression and some blood metabolites in dairy cattle during the transition period were performed. Blood samples (5 mL) were obtained from the tail vein of 100 cows with fourth and fifth lactation one week pre-parturition and one week post-parturition. In the present study, It was found positive correlations between plasma NEFA concentrations in the pre-parturition period and BHBA, GGT, MDA, and NF-κB and negative correlations between plasma NEFA concentration and HDL. As well as, the positive correlations between BHBA plasma concentration and NEFA , ALP, GGT, MDA and NF-κB and negative correlations between plasma BHBA concentration and HDL in the post-parturition period. Also while the cut-off point BHBA≤1.2(Health) and BHBA≥ 1.2(mmol/l)(subclinical ketosis or disease) in the post-parturition period was designated, it was observed that NEFA(p<.0001), BHBA(p<.0001), MDA(p<.0001), GGT(p<.0001) and ALP(p=0.03) were gradually increased, and HDL(p= <.0001) gradually decreased in disease group. The results showed that the activity of NF-κB (p<.0001) was gradually increased in the disease group. Also results showed that increased NEFAs and BHBA increased inflammation response through activating the NF-κB pathway and oxidative stress. Manuscript profile
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  4 - Study of the levels of beta hydroxy butyrate, glucose, protein and albumin in Holstein cows with subclinical ketosis
  بهرام Amouoghli Tabrizi شهاب‌الدین Safi سیامک Asri Rezaee مهدی Abdie Nojamehr
    The objective of this study was to comparatively evaluate the levels of beta hydroxy butyrate (BHB), glucose, protein and albumin in serum of healthy Holstein cows and those with subclinical ketosis. In this survey, blood samples were collected at two stages from cow More

    The objective of this study was to comparatively evaluate the levels of beta hydroxy butyrate (BHB), glucose, protein and albumin in serum of healthy Holstein cows and those with subclinical ketosis. In this survey, blood samples were collected at two stages from cows selected at 7 dairy farms in Shahriar province of Tehran. Five to 7 ml of blood were taken from the coccygeal vein of 100 cows during the last week of pregnancy when the animals were dry and once again 2 months after parturition from the same cows, their sera separated and the amounts of BHB, glucose, protein and albumin determined by enzymatic techniques and commercially available kits. With the cut point of BHB at 1.2, 1.4 and 1.7 mmol/lit, the percentage of cows affected with subclinical ketosis were 18, 14 and 4 percent, respectively. Mean levels of BHB in ketotic cows was significantly higher than healthy cows before and after parturition while mean levels of glucose, protein and albumin was significantly lower during the same periods (P Manuscript profile
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  5 - Assessment of thyroid hormones, insulin and magnesium in dairy cattle with subclinical ketosis
  علی Sadeghi-nasab علی Hassanpour حبیب Sabaghsaray سعید Amiri- Sadeghan
  Ketosis, with ethologically energy deficiency, is one of the commonest metabolic diseases of dairy cattle. Assessment of some serum hormonal and metabolic status can be beneficent in prediction and diagnosis of subclinical ketosis. This study is performed for evaluation More

  Ketosis, with ethologically energy deficiency, is one of the commonest metabolic diseases of dairy cattle. Assessment of some serum hormonal and metabolic status can be beneficent in prediction and diagnosis of subclinical ketosis. This study is performed for evaluation and comparison of thyroid hormones, insulin and magnesium of subclinical ketosis and healthy cows. During 60 days in milk (DIM) serum Beta hydroxy butyric acid (BHBA) and glucose levels of 200 dairy cattle have been measured. Based on 1.4 mmol/l of BHBA as a cut of point experimented animals separated to subclinical and healthy groups. Mean values of serum T3 and T4 of affected cows were significantly higher than non-affected (p<0.05). On the other hand, magnesium and insulin level of subclinically cases were significantly lower than control group (p<0.01 and p<0.05 respectively). There was significant correlation coefficient between BHBA and insulin levels of ketosis cows (p<0.05, r=0.37). In conclusion, increasing levels of thyroid hormones and decreasing magnesium and insulin of subclinically affected cattle could be used for diagnosis and prediction of this kind of ketosis at the herd level.   Manuscript profile
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  6 - Evaluation of the levels of Leptin, Beta hydroxyl butyrate, Glucose, Cholesterol and Triglyceride in serum of Holstein cows with sub clinical ketosis
  سیامک Asri Rezayi بهرام Amouoghli Tabrizi بابک Saber Marouf
  Leptin hormone is secreted from the white adipocytes of adipose tissue and its levels increase with the increase in size of the adipose tissue.  One of the most important actions of this hormone is the regulation of body metabolism by consuming adipose tissue and p More

  Leptin hormone is secreted from the white adipocytes of adipose tissue and its levels increase with the increase in size of the adipose tissue.  One of the most important actions of this hormone is the regulation of body metabolism by consuming adipose tissue and production of energy. The objective of this study was determination of Leptin and BHB, glucose, cholesterol and triglyceride levels in healthy Holstein cows and cows with Sub clinical Ketosis and the determination of the prevalence of Sub clinical Ketosis, using BHB level in blood serum as the gold standard. In this survey 7 dairy farms were chosen in Shahriar, (Tehran province), Samples were taken from 100 cows at two periods: 1) last week of pregnancy (dry period), 2) The same cows at 2 months after parturition. Serum samples were harvested and leptin levels were measured using DBC ELISA kit,Canada, BHB levels  were measured using  RANBUT kits and glucose, cholesterol and triglyceride levels were measured by commercial  kits (ziest chem) and spectrophotometer. In this study, the prevalence of Sub clinical Ketosis, using the 1/2, 1/4, 1/7 mmol BHB, as the cut point was calculated as 18%, 14% and 4% respectively. Leptin levels decreased significantly after parturition in healthy cows and those affected by subclinical ketosis. There was a significant correlation between leptin and glucose (r=0.53) and BHB and glucose (r= -0.27) in pre parturient group of cows. In the group of cows 2 month after parturition, there was a significant correlation between leptin and glucose (r=0.65), BHB and triglyceride (r=0.97) and BHB and glucose (r= -0.64). In the group affected by subclinical ketosis, a significant correlation was observed between leptin and glucose (r=0.72), BHB and glucose (r=-0.38), BHB and triglyceride (r=0.85) and BHB and cholesterol (r=0.64). Manuscript profile
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  7 - Evaluation of sensitivity and specialty of milk BHBA strip in comparison with blood BHBA in purpose of detection of subclinical ketosis in dairy herds
  بابک Bastani, A. Shaghayegh, فرهاد Moosakhani,
  Ketosis is a common metabolic disorder frequently observed in dairy cows during early lactation period, it ischaracterized by increased levels of ketone bodies in blood, urine and milk. Sub clinical ketosis (SCK) in dairycattle is an excess level in circulating ketone b More

  Ketosis is a common metabolic disorder frequently observed in dairy cows during early lactation period, it ischaracterized by increased levels of ketone bodies in blood, urine and milk. Sub clinical ketosis (SCK) in dairycattle is an excess level in circulating ketone bodies in the absence of clinical signs of ketosis. In this study,cow side strip test is studied for sensitivity ,specialty, advantages and disadvantages in relationship with serumBHBA (Gold standard test).32 multiparus cows were used in this research. All diets were based on TMR. Allcows were sampled during 7-21 DIM. Cows with serum BHBA concentration over 1400μmol/lit and milk BHBAconcentration over x>100µmol/lit considered to suffer from subclinical ketosis. in this cut point, sensitivity andspecialty of milk BHBA were 42.2% and 100%. kappa and p-value were 0.68 and 0.0005(x<0.05).according to theinformation above we can not denote this test for detection of subclinical ketosis in dairy herds but we can advisethis cow side test as an individual test. Manuscript profile