Determination of Diagnostic Value of Novavet ketone meter in the diagnosis of cows with subclinical ketosis

Subject Areas : Journal of Comparative Pathobiology

M.R  Taheri Jazi ¹ (D.V.M. Graduate, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran)
maryam  karimi dehkordi ² (Department of clinical Sciences, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran)

Received: 2021-11-03 Accepted : 2021-11-03 Published : 2020-12-21

Keywords: Novavet ketone meter, Subclinical ketosis, Dairy cow,

Abstract :

One of the most important metabolic abnormalities in the early lactation period is ketosis. The gold standard test for the diagnosis of ketosis is the measurement of beta-hydroxybutyrate (BHB) in the blood. In this study, the diagnostic value of the electronic ketone meter, Novavet, for diagnosing cows with ketosis was evaluated in comparison with the standard laboratory method. Blood samples were taken from 68 cows in the period of 7-14 days after calving. In the laboratory, serum BHB was measured by spectrophotometry and Randox kit.  At the time of blood sampling, the amount of BHB in whole blood was determined with ketone meter in the same cows. BHB values obtained from the two methods were analyzed by T-test and Pearson correlation statistical models. In order to determine the best cut points of BHB using Novavet machine, which can make the best distinction between healthy and ketosis cows (based on reference test), also to estimate the sensitivity and specificity of Novavet, the ROC analysis is used. The amount of BHB in whole blood in the manual method is significantly higher than the laboratory method. However, there is a significant and good correlation between these two methods. Also, Novavet at the cut point of 1.2 mmol/l has the highest combination of sensitivity and specificity (100 and 41.9%) compared to the standard test. Due to the good and significant correlation between the Novavet test and its high sensitivity, it can be said that this method has an acceptable accuracy for diagnosing hyperketonemic cows.

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