Study of the levels of beta hydroxy butyrate, glucose, protein and albumin in Holstein cows with subclinical ketosis

Subject Areas : Veterinary Clinical Pathology

بهرام Amouoghli Tabrizi ¹ , شهاب‌الدین Safi ² , سیامک Asri Rezaee ³ , مهدی Abdie Nojamehr ⁴

1 - Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Tabriz, Tabriz, Iran.
2 - Department of Clinical Sciences, Faculty of Specialized Veterinary Sciences, Islamic Azad University, Science & Research Campus, Tehran, Iran.
3 - Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
4 - Department of Food Hygiene, Faculty of Veterinary Medicine, Islamic Azad University of Tabriz, Tabriz, Iran.

Received: 2007-09-09 Accepted : 2007-11-11 Published : 2007-08-23

Keywords: Protein, glucose, albumin, subclinical ketosis, beta hydroxy butyrate,

Abstract :

  The objective of this study was to comparatively evaluate the levels of beta hydroxy butyrate (BHB), glucose, protein and albumin in serum of healthy Holstein cows and those with subclinical ketosis. In this survey, blood samples were collected at two stages from cows selected at 7 dairy farms in Shahriar province of Tehran. Five to 7 ml of blood were taken from the coccygeal vein of 100 cows during the last week of pregnancy when the animals were dry and once again 2 months after parturition from the same cows, their sera separated and the amounts of BHB, glucose, protein and albumin determined by enzymatic techniques and commercially available kits. With the cut point of BHB at 1.2, 1.4 and 1.7 mmol/lit, the percentage of cows affected with subclinical ketosis were 18, 14 and 4 percent, respectively. Mean levels of BHB in ketotic cows was significantly higher than healthy cows before and after parturition while mean levels of glucose, protein and albumin was significantly lower during the same periods (P

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