Evaluation of sensitivity and specialty of milk BHBA strip in comparison with blood BHBA in purpose of detection of subclinical ketosis in dairy herds

Subject Areas : clinical veterinary science

بابک Bastani, ¹ ( Faculty of veterinary medicine, Karaj branch , Islamic azad university ,Karaj, Iran )
A. Shaghayegh, ² ( Department of clinical sciences ,Faculty of veterinary medicine, Karaj branch, Islamic azad university, Karaj ,Iran )
فرهاد Moosakhani, ³ ( Department of pathobiology, Faculty of veterinary medicine, Karaj branch, Islamic azad university, Karaj, Iran )

Received: 2016-11-03 Accepted : 2016-11-03 Published : 2013-12-22

Keywords: dairy cow, subclinical ketosis, cow side tests, milk BHBA, serum BHBA,

Abstract :

Ketosis is a common metabolic disorder frequently observed in dairy cows during early lactation period, it ischaracterized by increased levels of ketone bodies in blood, urine and milk. Sub clinical ketosis (SCK) in dairycattle is an excess level in circulating ketone bodies in the absence of clinical signs of ketosis. In this study,cow side strip test is studied for sensitivity ,specialty, advantages and disadvantages in relationship with serumBHBA (Gold standard test).32 multiparus cows were used in this research. All diets were based on TMR. Allcows were sampled during 7-21 DIM. Cows with serum BHBA concentration over 1400μmol/lit and milk BHBAconcentration over x>100µmol/lit considered to suffer from subclinical ketosis. in this cut point, sensitivity andspecialty of milk BHBA were 42.2% and 100%. kappa and p-value were 0.68 and 0.0005(x<0.05).according to theinformation above we can not denote this test for detection of subclinical ketosis in dairy herds but we can advisethis cow side test as an individual test.