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Open Access Article

1 - Evaluation of serum biochemical profile using black radish (Raphanus sativus) in mice
Seyedeh Ommolbanin Ghasemian Maryam Maryam Karimi-Dehkord Ali Maddahi nejad Hooman Yousefi

Background & Aim: Considering the presence of active compounds in black horseradish, the present study aimed to evaluate the effect of hydroalcoholic extract of black radish on liver and kidney function in small laboratory mice. Materials & Methods: In this study, 40 s More

Background & Aim: Considering the presence of active compounds in black horseradish, the present study aimed to evaluate the effect of hydroalcoholic extract of black radish on liver and kidney function in small laboratory mice. Materials & Methods: In this study, 40 small laboratory mice were randomly divided into four groups of control and experimental groups (50, 100, and 150 mg/ml black radish extract). Intraperitoneal injection of hydroalcoholic extract of black radish was performed in the experimental groups. On the 21st day, the mice were anesthetized and then blood sampling was done. Blood urea nitrogen (BUN), creatinine, alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), triglyceride and cholesterol levels were compared between the groups. Results: The levels of BUN, creatinine, ALP, AST and ALT in the experimental groups were not significantly different from the control group (P>0.05). Treatment with horseradish extract led to a decrease in fat profiles in the experimental group compared to the control group. By increasing the dose of the extract, the amount of triglycerides decreased. This reduction was significant in the highest dose (150 mg/kg) (P<0.05). In addition, black radish extract led to a significant decrease in cholesterol in all three doses compared to the control group (P<0.05). Conclusion: The results of the present study indicate the absence of liver and kidney toxicity of the extract in the doses used in the study. It can also be said that in physiological conditions, the use of black horseradish hydroalcoholic extract reduces the fat profile Manuscript profile

Open Access Article

2 - Effect of Oilseed Radish (Rhaphanus sativus oleifera) Rotation as a Trap – Plant on Quantitative and Qualitative Characteristics of Sugar Beet (Beta vulgaris) and Reduce the Severity of Rhizomania Infection
Mastaneh Sharifi Saeid Sadeghzadeh Hemayati Gholam Reza Ashrafmansoori

In order to evaluate the rotation potential of different cultivars of oilseed radish (Rhaphanus sativus oleifera) named Adagio, Luna, Colouel and Accent on quantitative and qualitative Characteristics of Sugar Beet (Zarghan cultivar) and reduction of rhizomania infectio More

In order to evaluate the rotation potential of different cultivars of oilseed radish (Rhaphanus sativus oleifera) named Adagio, Luna, Colouel and Accent on quantitative and qualitative Characteristics of Sugar Beet (Zarghan cultivar) and reduction of rhizomania infection severity, an experiment was conducted using Latin Square Design with five replications in Zarghan station in 2009-2011 in Fars province. The treatments were applied in first year as 1- no trap plant (check), 2- Adagio, 3- Luna, 4- Colouel, 5- Accent, and in second year Zarghan cultivar was planted in the same plots. Quantitative and qualitative traits and rhizomania infection severity measured after harvest. Results of compound analysis showed that the effect of rotation with trap plant cultivars on qualitative traits in Zarghan cultivars was not significant , but based on Friedman test, rhizomania infection severity in cultivated plots with trap plant was significant in 5% probability level so that in plots that Luna and Accent  had been cultivated mean of disease degree was 1.85 and 2.5, respectively, that was less than other treatments. The biomass produced in the trap plant Luna and Accent less than other cultivars and their impact on disease reduction was remarkable. The effect of Trap plant compared to control treatment (without traps planted) on root yield and sugar yield was very evident in Zarghan comparison to control that root and sugar yield was reduced. In the control plot (without trap plant) the lowest root yield (36.65 t/ha) and sugar yield (6.28 t/ha) were included in separate groups. Acording to results it can be said that crop more than two years rotation with trap plant and using  relatively tolerant sugar beet cultivars, can use to decrease virus growth potential and increase quantity in sugar beet. Manuscript profile

Open Access Article

3 - Effects of seed heteroblasty on germination and seedling vigor in wild radish (Raphanus raphanistrum L.)
Ehsan Bijanzadeh Ali Behpoori

In order to investigate the effects of seed heteroblasty on germination and seedling vigor in wild radish (Raphanus raphanistrum L.) this study was conducted in Agricultural College of Darab in 2007. In laboratory and greenhouse, germination and seedling growth studies More

In order to investigate the effects of seed heteroblasty on germination and seedling vigor in wild radish (Raphanus raphanistrum L.) this study was conducted in Agricultural College of Darab in 2007. In laboratory and greenhouse, germination and seedling growth studies were conducted on seed harvested from different seed positions and heights of mother plant of wild radish by factorial in completely randomized design using four replications. Treatments consisted of three heights of mother plant (40-60, 60-80, 80-100 cm) and three seed positions within the inflorescence including: 1- Seeds in low part of inflorescence (seeds of the first to fifth siliceous in inflorescence), 2- Seeds in middle part of inflorescence (seeds of the sixth to tenth siliceous in inflorescence), 3- Seeds in upper part of inflorescence (seeds of the eleventh to fifteenth siliceous in inflorescence). The results in laboratory showed that among three heights of mother plant, the seeds of the sixth to tenth siliceous in inflorescence had the maximum germination percentage and produced the tallest length of radicle. Also, in height of 60-80 cm of mother plant, the seeds of sixth to tenth and first to fifth siliceous in inflorescence produced the tallest length of primary shoot. In greenhouse experiments, the seeds of  sixth to tenth  siliceous of inflorescence in height of 60-80 cm of mother plant produced the maximum number of plants and the seeds that harvested from sixth to tenth siliceous of  inflorescence in height of  80-100 cm of mother plant had the maximum height of wild radish. Manuscript profile

Open Access Article

4 - Enhancing bioavailability of lead for phytoremediation of contaminated soils
Zahra Arabi مهدی Homaee M.E Asadi

In this study the influence of enhancing synthetic chelate (HEDTA) were investigated on lead (Pb) solution in contaminated soils. The Pb phytoextraction capability of radish (Raphanus sativus L.) before and after chelating was also studied. The experiment was conducted More

In this study the influence of enhancing synthetic chelate (HEDTA) were investigated on lead (Pb) solution in contaminated soils. The Pb phytoextraction capability of radish (Raphanus sativus L.) before and after chelating was also studied. The experiment was conducted in a randomized complete factorial design, with three replicated for each treatment. The Pb treatments (as PbCl2) were consisted of 0 (control), 50, 100, 600 and 1000 mg Pb kg-1 soil. When plants were fully grown, the chelating agent HEDTA was added to Pb treatments in concentration of 1.5, 5 and 6 mMkg-1 soil, respectively. The control treatments was received no chelate. Ten days later, some samples were taken from the plants and soils to measure lead concentrations after applying the chelate. The results indicated that in all treatments, the concentrations of soluble Pb in soil were more than the control treatment. In all treatments, Pb concentrations in plant shoots and roots were increased by increasing Pb concentrations in the soil solution. In current study, Pb concentration in radish shoots and roots, after enhancing HEDTA was larger than control. The collected data were also showed that Pb uptake by radish roots was larger than that of shoots. Manuscript profile

Open Access Article

5 - Effect of Black Radish Hydroalcoholic Extract on Kidney and Functional Kidney in Balb/c Mice
Maryam Karimi Dehkordi Iman Jafari Guzel Rahbar Munira Shafahi Forough Mohammadi

10.22034/ascij.2023.1961079.1397

This study was performed to evaluate the effect of hydroalcoholic extract of black radish (Raphanus sativua) on complete blood cell count and kidney function in mice. Forty white mice were divided into four equal groups. Mice in the control group received only saline so More

This study was performed to evaluate the effect of hydroalcoholic extract of black radish (Raphanus sativua) on complete blood cell count and kidney function in mice. Forty white mice were divided into four equal groups. Mice in the control group received only saline solution and mice in the second to fourth groups received 50, 100 and 150 mg/kg body weight of black radish plant extract, respectively, by intraperitoneal injection. After a 21-day trial period, blood samples were taken from the heart to count complete blood cells (hematocrit, hemoglobin, red and white blood cells) and urea nitrogen and creatinine in serum were measured. The results showed that treatment with black radish extract in any of the studied doses did not cause a significant change in blood cell count and nitrogen urea and creatinine (p < 0.05). These results were confirmed by histopathological examination of the kidney. Treatment of mice with horseradish extract resulted in a non-significant decrease in blood urea nitrogen and creatinine compared to the control group. Also, with increasing the dose of the extract, the amount of urea nitrogen and creatinine in each group decreased compared to the previous group, although this decrease was not significant (p < 0.05). The results of the present study indicate the extract in the doses used in the study, is not nephrotoxic and it can be said that under physiological conditions, the use of hydroalcoholic extract of radish not only has no toxic effect on the kidney in high doses, but also has positive effects on kidney function. Manuscript profile

Open Access Article

6 - The effect of pretreatment of salicylic acid on radish (Raphanus sativus L.) seed germination under salt stress
Mojgan Ghanbari alireza eftekharian jahromi Shourangiz Javanmardi Mohsen Farzaneh

Optimal germination and plant establishment is an important consideration for agricultural productivity in arid and semi-arid areas. Priming is an approach for increasing plant establishment especially in undesirable conditions. To evaluate the effects of radish seeds p More

Optimal germination and plant establishment is an important consideration for agricultural productivity in arid and semi-arid areas. Priming is an approach for increasing plant establishment especially in undesirable conditions. To evaluate the effects of radish seeds priming with salicylic acid (SA) on seed germination indices and inducing tolerance to salt stress condition, this experiment was carried out. SA was applied as seed priming in 0, 1, 2 and 3 mM concentrations on radish seeds for 24 hours, then transferred to sterile Petri dishes and added 10 ml of NaCl solution with different concentrations including 0, 4, 8 and 12 dS/m. Germination rate and percentage, radicle and plumule length ratio, radicle / plumule weight ratio and proline content in radicle and plumule were recorded. Salinity decreased radish seed germination in high NaCl concentrations (12 dS/m). Application of SA increased germination percentage in stress and non-stress conditions. SA increased the plumule and radicle length ratio. However, 1 mM SA was the most effective treatment on the percentage and rate of seed germination. Proline content in plumule and radicle was increased under salt stress. Manuscript profile

Open Access Article

7 - Effect of salicylic acid on yield and osmolate content of radish (Raphanus sativus L.) at drought stress condition
Mojgan Ghanbari Mohsen Farzaneh Alireza Eftekharian Jahromi Shoorangiz Javanmardi

Possibility of reducing drought stress adverse effects on yield and osmolate content of radish (var. Radish cherry bell) by application a plant growth regulator, salicylic acid, was studied in Shiraz Branch, Islamic Azad University, Fars province, Iran in 2012. Experime More

Possibility of reducing drought stress adverse effects on yield and osmolate content of radish (var. Radish cherry bell) by application a plant growth regulator, salicylic acid, was studied in Shiraz Branch, Islamic Azad University, Fars province, Iran in 2012. Experiment was carried out as factorial based on a completely randomized design (CRD) with three replications under a long-term drought stress condition (45 days). Radish seeds were planted in pots with sandy-clay soil and drought stress was applied after two leaf stage. Factors were drought stress at three levels of one-day (normal), three-days (mild stress) and five-days (severe stress) irrigation intervals and salicylic acid at three levels (0, 1 and 2 mM). Yeild traits including length, diameter and weight of hypocotyl (under ground eatable part) and osmolates (prolin and soluble carbohydrates) were measured. The results showed that interaction effect of drought stress and salicylic acid was significant on all the studied traits. Sever stress condition caused a considerable reduction in hypocotyl length and diameter. Foliar application of salicylic acid 2 mM caused higher osmolate rates and it reduced the adverse effect of drought stress on radish yield. Manuscript profile

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