-
Open Access Article
1 - Tamali emphasizes the approach of Imamiyya jurisprudents by emphasizing the opponents of genetic actions
Sayyed Hassan Mortazavi Rad Masoud Raei Dehaghi Reza Abbasian Hamid Reza NikyarThe emergence of genetic science and the research on the human genome and the new functions that make up this new subject have created a range of discussions between experts and jurisprudents, and since jurisprudence has the ability to respond to the issues that are und MoreThe emergence of genetic science and the research on the human genome and the new functions that make up this new subject have created a range of discussions between experts and jurisprudents, and since jurisprudence has the ability to respond to the issues that are under investigation, This issue is also necessary for the views of the jurists to be considered. Referring to the views expressed by the jurisprudents, there are various statements ranging from consent to opposition to such actions. Among these, the arguments for the opposition to express their opposition to the particular effects on it and the type of reasoning presented are of particular importance. . What this research seeks to answer is, on the one hand, to explain the approach of jurists to this issue and, on the other hand, to discover why some scholars oppose genetic research. Findings of the research indicate that the reasons for the adversaries have a stronger position, although there are disagreements among the adherents, and what is presented as a reason for opposition from the opponents of genetic actions is a legal concern, rather than a reason, Because research on the human genome is not in the limelight in the form of initial titles, and if the issue of abusing the permits of such actions is posed, it is another issue that opponents follow from the interpretation of the Convention that has the negative consequences of such actions Emphasizing that by preventing the potential misuse of this There is a background and there is more evidence that the arguments of the opponents of genetic actions can be ruled out. Manuscript profile -
Open Access Article
2 - Comparison of structure and genetic diversity of Common Pochard (Aythya ferina) using cytochrome B mitochondrial gene in northern and southern regions of the country
Shabnam chavoshi Jalil Imani hamidReza Rezaei Pargol Ghavam MostafavBackground & Objective: The Common Pochard (Aythya ferina) is one of the most abundant and widely distributed species in the world, among ducks. The aim of this study was to investigate the structure and genetic diversity of Aythya ferina in Iran using cytochrome b MoreBackground & Objective: The Common Pochard (Aythya ferina) is one of the most abundant and widely distributed species in the world, among ducks. The aim of this study was to investigate the structure and genetic diversity of Aythya ferina in Iran using cytochrome b gene. Material and Methodology: For this study, samples were taken from 10 pieces of Aythya ferina that were taken from the north and south of the country. Haplotypic and nucleotide analyzes were performed using MEGA, Papart, DNAsp and Network software. Findings: In general, 7 different haplotypes were identified by examining 8 fragments of 929 pairs of cytochrome b gene of Aythya ferina. Haplotype diversity was calculated 0.818, nucleotide diversity was calculated 0.00149. The value of Nm or the amount of gene flow between the sampling logic was 1.383, which indicates a high gene flow and in other words, a very small gene distance between the populations of this species and its European and Asian populations. Rogers-Harping index, Tajima index and Fu Fs were calculated 0.112, -0.028 and -1.288, respectively. The mismatch distribution diagram is also plotted as a single view, all of which show an indeterminate population history similar to the model of sudden expansion. Discussion and Conclusion: According to the results obtained from the haplotype network and the cognitive tree, as well as a slight genetic difference between the studied populations, there is a possibility of migration of this bird from both Europe and East Asia to Iran. Considering the results of the present study, Iranian wetlands should be recognized as important and key areas and the necessary management and protection measures should be taken in these areas. Manuscript profile -
Open Access Article
3 - CRISPR System from Encounter with a Mysterious Repeated Sequence to Genome Editing Technology
Mojtaba Sohrabi Zahrasadat Monzavi zahra abdolahi hamed salmani somayeh dehghani sanij Abbas MorovvatiClustered regularly interspaced short palindromic repeat (CRISPR)- Cas systems are well-known acquired immunity systems that are widespread in archaea and bacteria. The RNA-guided nucleases from CRISPR-Cas systems are currently regarded as the most reliable tools for ge MoreClustered regularly interspaced short palindromic repeat (CRISPR)- Cas systems are well-known acquired immunity systems that are widespread in archaea and bacteria. The RNA-guided nucleases from CRISPR-Cas systems are currently regarded as the most reliable tools for genome editing and engineering. The first hint of their existence came in 1987, when an unusual repetitive DNA sequence, which subsequently was defined as a CRISPR, was discovered in the Escherichia coli genome during an analysis of genes involved in phosphate metabolism. Similar sequence patterns were then reported in a range of other bacteria as well as in halophilic archaea, suggesting an important role for such evolutionarily conserved clusters of repeated sequences. A critical step toward functional characterization of the CRISPR-Cas systems was the recognition of a link between CRISPRs and the associated Cas proteins, which were initially hypothesized to be involved in DNA repair in hyperthermophilic archaea. Comparative genomics, structural biology, and advanced biochemistry could then work hand in hand, not only culminating in the explosion of genome editing tools based on CRISPR-Cas9 and other class II CRISPR-Cas systems but also providing insights into the origin and evolution of this system from mobile genetic elements denoted casposons. To celebrate the 30th anniversary of the discovery of CRISPR, this minireview briefly discusses the fascinating history of CRISPR-Cas systems, from the original observation of an enigmatic sequence in E. coli to genome editing in humans. Manuscript profile -
Open Access Article
4 - The genetic structure of geographic populations of Chilo suppressalis (Walker) (Lepidoptera: Crambidae) in northern Iran based on mitochondrial COII gene
Abbas Heydari Alireza Nazari Mohammad Ali Oshaghi Elham SanatgarRice is one of the most important and oldest crop and is one of the three leading crops in the world. Chilo suppressalis (Walker), rice striped stem borer, is a key insect pest damaging the rice crop up to 33 percent. Understanding genetic structure of the pest is essen MoreRice is one of the most important and oldest crop and is one of the three leading crops in the world. Chilo suppressalis (Walker), rice striped stem borer, is a key insect pest damaging the rice crop up to 33 percent. Understanding genetic structure of the pest is essential for an effective pest management program and can corporate with control program. Aim of this stud was to determine the genetic structure of mitochondrial COII (mtDNA-COII) gene of the pest in north of Iran. Rice stem borer specimens were collected from rice fields in Guilan and Mazanderan provinces, at Caspian Sea coast at seasonal activity (spring) in 2016-2017. Genetic structure of 18 populations of the species was invesigated using polymerase chain reaction (PCR) followed by direct-sequencing of mtDNA-COII gene. The obtained sequences were compared with each other and the available data in GenBank database. In total, 312 and 676 specimens were collected from Guilan and Mazanderan provinces respectively. Results showed that all specimens from two provinces are identical are like specimen from South Korea with GenBank ID MK207057. Manuscript profile -
Open Access Article
5 - Comparing qualitative and quantitative results of some DNA extraction methods from genome of pathogenic and saprophytic mycobacteria
Ali Nasiri Younes Anzabi Mohammad reza MashayekhiGiven that the first step in performing molecular techniques such as PCR (Polymerase Chain Reaction (is having enough high quality DNA, the aim of this study was to compare five different extraction methods of genomic DNA of mycobacteria in order to introduce the best m MoreGiven that the first step in performing molecular techniques such as PCR (Polymerase Chain Reaction (is having enough high quality DNA, the aim of this study was to compare five different extraction methods of genomic DNA of mycobacteria in order to introduce the best method. For this purpose, pure colonies of Mycobacterium avium paratuberculosis were used as pathogenic mycobacterium and also a saprophytic strain called Mycobacterium phelei. The quality and quantity of the obtained DNA by each method was calculated, measured and evaluated respectively; based on extracted DNA concentrations (nanograms per microliter) at a wavelength of 260 nm and also optical density (OD) ratio based on the 260/280 wavelength by using the Nanodrop technique. The highest and lowest amounts of mycobacterial genome DNA was extracted using the CTAB (Cetyltrimethylammonium bromide) and boiling methods respectively. Comparison of the purity of extracted DNA also indicated that the highest purity was obtained using the CTAB method and the lowest using the boiling method. Also no significant differences were observed regarding the quality of extracted DNA from pathogenic and saprophytic mycobacteria among the evaluated extraction methods. Thus, according to the results of this study it appears that using the CTAB method is the most suitable way to extract genomic DNA for molecular detection of mycobacteria in different samples. Manuscript profile -
Open Access Article
6 - The Human Genome Project with an Approach to the Ethical Perspective and Human Freedom
Amirreza Mahmoudi Abbas Taghvaee Seyedeh Mahshid Miri BalajorshariIn this research, the results of the Human Genome Project (HGP) and the possible ethical problems it may cause were evaluated through concrete examples. The study has tried to reveal in detail how the social rights and freedoms, ethics, social norms, and values of the i MoreIn this research, the results of the Human Genome Project (HGP) and the possible ethical problems it may cause were evaluated through concrete examples. The study has tried to reveal in detail how the social rights and freedoms, ethics, social norms, and values of the individual will be affected and where the borders will begin and end in the event of the potential risks that HGP carries. Discussions have been made on the problem that the individual can be instrumentalized and turned into a commodity as a result of genetic interventions, and unfortunately, it has been determined that genetic interventions are in a structure that can lead to an intervention in the fundamental rights and freedoms of the individual. This study aims to show the usage areas of the human genome project and to examine the results of this project in terms of the ethical consequences of the techniques and applications used. Manuscript profile -
Open Access Article
7 - برآورد اندازه مؤثر جمعیت و ضرایب همخونی ژنومی در گوسفند نژاد بلوچی با استفاده از تراشه های متراکم نشانگری
م. پسندیده م. قلی زاده ق. رحیمی میانجیهدف از این مطالعه، بررسی اندازه مؤثر جمعیت و میزان همخونی ژنومی در 96 رأس گوسفند بلوچی با استفاده از اطلاعات نشانگرهای SNP موجود در سراسر ژنوم حاصل از تراشه متراکم است. با استفاده از نرم ­افزار NEESTIMATOR، اندازه مؤثر کنونی جمعیت براساس روش­ های هتروزیگوسیتی اض Moreهدف از این مطالعه، بررسی اندازه مؤثر جمعیت و میزان همخونی ژنومی در 96 رأس گوسفند بلوچی با استفاده از اطلاعات نشانگرهای SNP موجود در سراسر ژنوم حاصل از تراشه متراکم است. با استفاده از نرم ­افزار NEESTIMATOR، اندازه مؤثر کنونی جمعیت براساس روش­ های هتروزیگوسیتی اضافی و LD به ترتیب 15 و 25 تخمین زده شدند. نتایج به دست آمده از اندازه مؤثر تاریخی جمعیت با استفاده از نرم­افزار SNeP، نشان دهنده یک روند کاهشی سریع در طول 1000 نسل گذشته بود. اندازه Ne در چهار نسل قبل در دامنه 75-48 رأس بود. ضریب همخونی با استفاده از سه روش ماتریس روابط خویشاوندی (FGRM)، میزان هموزیگوسیتی (FHOM)و همبستگیگامت­ها (FUNI)با استفاده از نرم­افزارGCTA محاسبه شدند. مقادیر FGRM، FHOM وFUNI به­طور مشابه برابر با 0.017- محاسبه شدند. میانگین هتروزیگوسیتی مورد انتظار و مشاهده شده با استفاده از نرم­افزارPLINK به ترتیب 0.374 و 0.383 برآورد شدند. نتایج این تحقیق نشان داد که تنوع ژنتیکی مناسبی در نژاد بلوچی وجود دارد اما مقادیر پایین Ne بیانگر اجرای اقدامات ویژه ­ای برای جلوگیری از کاهش شدیدتر اندازه مؤثر جمعیت در این نژاد است. بنابراین طراحی برنامه­ های مناسب برای حفاظت از حیوانات خالص این نژاد ضروری است. Manuscript profile -
Open Access Article
8 - The Complete Mitochondrial Genome from Iraqi Meriz Goats and the Maternal Lineage Using Whole Genome Sequencing Data
S.I. Mustafa J.S. Heslop-Harrison T. Schwarzacher -
Open Access Article
9 - Spermatozoa Molecules in Relation to Bulls Fertility
اس.آ. لون ر. سینها آ. رحیم ب.آ. جانایی آ. سینق ن. شاه -
Open Access Article
10 - Genome Wide Association Studies, Next Generation Sequencing and Their Application in Animal Breeding and Genetics: A Review
ح. خانزاده ن. قوی حسین-زاده ش. قوتی -
Open Access Article
11 - پیشبینی جزایر CpG و بررسی ارتباط آنها با ویژگیهای ژنومی با استفاده از مدل مخفی مارکوف در گاو
A. برازنده م.ر. محمدآبادی م. قادری ح. نظام آبادی پورگاو یکی از مهمترین تأمین کننده­های مواد غذایی و مغذی بشر در جهان است. جزایر CpG نواحی مهم و مفیدی هستند که دارای ارتباط عملکردی با رویداد­های مهم اپی ژنتیکی در سطح ژنوم می­باشند. با توجه به اینکه تاکنون جزایر CpG در سطح ژنوم گاو بررسی نشده­اند هدف از این Moreگاو یکی از مهمترین تأمین کننده­های مواد غذایی و مغذی بشر در جهان است. جزایر CpG نواحی مهم و مفیدی هستند که دارای ارتباط عملکردی با رویداد­های مهم اپی ژنتیکی در سطح ژنوم می­باشند. با توجه به اینکه تاکنون جزایر CpG در سطح ژنوم گاو بررسی نشده­اند هدف از این مطالعه بررسی این جزایر در ژنوم گاو با استفاده از مدل­های محفی مارکوف است. تعداد 90668 جزیره CpG در ژنوم گاو پیش­بینی گردید. تغییرات تعداد و تراکم جزایر CpG در طول کروموزوم­ها بسیار کم بود. کروموزوم 25 دارای بیشترین تعداد (4556) و تراکم (CGIs/Mb 106٫20) جزایر CpG بود. همبستگی مثبت و معنی­دار بین تراکم جزایر CpG با محتوی GC، نسبت مشاهده شده به مورد انتظار CpG، نرخ نوترکیبی و تراکم ژن مشاهده گردید. با افزایش سایز کروموزم­ها تراکم جزایر CpG کاهش یافت و همچنین تراکم جزایر CpG در نواحی تلومری کروموزم­ها بیشتر از سایر قسمت­ها مشاهده گردید که می­تواند علت همبستگی مثبت بین نرخ نوترکیبی و تراکم جزایر CpG باشد. جهت یافتن تفاوت­های جزایر CpG بین ژنوم گاو و سایر مهره­داران جزایر CpG در یازده مهره­دار دیگر بررسی گردید. تغییرات تراکم جزایر CpG در بین حیوانات مورد مطالعه بسیار متفاوت بود. یافته­های این مطالعه می­تواند در درک بهتر نقش اپی ژنتیکی و نقش تکامل مولکولی جزایر CpG در ژنوم گاو مؤثر باشد. Manuscript profile -
Open Access Article
12 - Genetic Analysis of Three Structural Proteins in Iranian Infectious Bronchitis Virus Isolate
M. Nosrati M. Tahmorespoor M.R. Nassiry -
Open Access Article
13 - Genomic characterization and phylogenetic analysis of a narrow host-range Iranian strain of Xanthmonas citri sub. citri, NIGEB-88
Amir Jalali Seyed Mehdi Alavi Mohammad Hossein SangtarashBackground & Objectives: Xanthomonas citri subsp. citri (Xcc) is the causal agent of citrus bacterial canker. In Iran, for the first time, Alizadeh and Rahimian reported the presence of Xcc on Mexican lime (C. aurantifolia), in 1990. Early characterization data sugg MoreBackground & Objectives: Xanthomonas citri subsp. citri (Xcc) is the causal agent of citrus bacterial canker. In Iran, for the first time, Alizadeh and Rahimian reported the presence of Xcc on Mexican lime (C. aurantifolia), in 1990. Early characterization data suggested the presence of both wide (pathotype A) and narrow (pathotype A* and Aw) host range Xcc strains in Iran. However, more recently a discriminant analysis of MLVA-31 and MLVA-14 data in 2014 showed that Iranian strains of Xcc have genetically belonged to the host-restricted pathotype A* but not to the pathotype A. This study was aimed for genomic characterization and phylogenetic analysis of a narrow-host-rang Iranian strain of X. citri sub. citri, NIGEB-88.Materials & Methods: To determine genetic characteristics and phylogenetic relationships of Iranian strains, Illumina sequencing method was used to obtain a draft genome sequence of Xcc strain NIGEB-88.Results: General features of Iranian strain such as genome size, number of plasmids, average genomic GC content, number of CDS and structural RNA were similar to those of other Xcc pathotypes. Furthermore, studies of various potential virulence and host range determinants factors such as type III secretion system effectors, type IV secretion system and surface Lipopolysaccharides revealed that this pathogen is very close to XccA and XccAw strains.Conclusion: Phylogenetic and assessment of genes related to virulence and host specificity showed that XccA*NIGEB-88 is more closely related to other Iranian strain, XccA*NIGEB-386, and narrow-host-rang pathotype A*. Manuscript profile -
Open Access Article
14 - Molecular Analysis of Najdi Goat Population Using HVR1 Sequence of Mitochondrial Genome
Rouhollah Khademi Seyedeh Ommolbanin Ghasemian Hamid Reza Seyyed Abadi amin kazemizadehThis study was conducted to determine the sequence of the HVR1 region of the mitochondrial genome of the Najdi goat. To conduct this study, 30 blood samples of both gender were collected from unrelated goats. After DNA extraction, the desired region was amplified by spe MoreThis study was conducted to determine the sequence of the HVR1 region of the mitochondrial genome of the Najdi goat. To conduct this study, 30 blood samples of both gender were collected from unrelated goats. After DNA extraction, the desired region was amplified by specific primers by PCR technique, and the sequence was determined. For comparison, the phylogeny of the HVR1 region sequence obtained from Najdi goat was drawn with other breeds worldwide to determine the haplotype group. The phylogenetic tree drawn for the samples showed that they all originated from the same population and the number of 5 different haplotypes was determined based on 20 nucleotide polymorphisms (SNP) for the HVR1 region in the sequences. Also, the sequence of the HVR1 region of the studied sample with 11 sequences recorded from 6 haplotype groups from different countries in the NCBI database showed that the Najdi goat belongs to haplotype group A. Comparing the sequence of the HVR1 region with the sequences in the gene bank can contribute to our information about the Najdi goat breeds and open the ground for their better use in breeding programs. According to the obtained results, the genetic diversity of the Najdi goat has increased over many years, and this increase in genetic diversity can be due to the mixing of this breed with other breeds, which can lead to the extinction of the Najdi goat in the future, which requires more attention to this issue. Manuscript profile -
Open Access Article
15 - Genome-Wide Scanning for Signatures of Selection Revealed Karakul Sheep Breed in Compared to other Iranian Breeds
A. Mirzapour-Abibagloo N. Hedayat R. Khalkhali-Evrigh R. Seyedsharifi H. Abdi-Benemar R. Hassanzadeh A. Tanveer HussainKarakul (KAR) is one of the resistant sheep breeds to harsh desert conditions, which is also known for its excelent lamb pelt quality. This study was performed to identify the signature of selections in the KAR breed using whole-genome sequencing data (WGS) compared wit MoreKarakul (KAR) is one of the resistant sheep breeds to harsh desert conditions, which is also known for its excelent lamb pelt quality. This study was performed to identify the signature of selections in the KAR breed using whole-genome sequencing data (WGS) compared with five other Iranian native sheep. Three methods, including population differentiation index (Fst), nucleotide diversity (π), and cross-population extended haplotype homozygosity (XP-EHH) applied to detect the genomic signature of selection. Data analysis leads to identifying 38 shared genes among three methods as positively selected genes for the KAR breed. The most of mentioned genes were associated with coat color (KIT, DVL3, YPEL3, ERBB4, ZNF451, and CTSO), fat and energy metabolism (GDPD3, STARD13, ZNF106, MAPK3, RGS6, PHYH, AP2M1, SPAG9, DNAH9, NDUFAF6, and ARSK), muscle function (MYOCD and MCTP1), growth (CPNE4), altitude adaptation (DNAH9 and SERGEF), and reproduction (TBX6, PHYH, SPAG9, and ARSK). Based on our results, these candidate genes may have a positive effect on the adaptation of the KAR breed to a desert environment. Manuscript profile
Sanad
Sanad is a platform for managing Azad University publications
Links
Related Centers
Technical Support
Official pages
The rights to this website are owned by the Raimag Press Management System.
Copyright © 2021-2024