برآورد اندازه مؤثر جمعیت و ضرایب همخونی ژنومی در گوسفند نژاد بلوچی با استفاده از تراشه های متراکم نشانگری
Subject Areas : Camelم. پسندیده 1 , م. قلی زاده 2 , ق. رحیمی میانجی 3
1 - Department of Animal Science, Laboratory for Molecular Genetics and Animal Biotechnology, Faculty of Animal and Aquatic Science, Sari Agricultural Science and Natural Resources University, Sari, Iran
2 - Department of Animal Science, Laboratory for Molecular Genetics and Animal Biotechnology, Faculty of Animal and Aquatic Science, Sari Agricultural Science and Natural Resources University, Sari, Iran
3 - Department of Animal Science, Laboratory for Molecular Genetics and Animal Biotechnology, Faculty of Animal and Aquatic Science, Sari Agricultural Science and Natural Resources University, Sari, Iran
Keywords:
Abstract :
هدف از این مطالعه، بررسی اندازه مؤثر جمعیت و میزان همخونی ژنومی در 96 رأس گوسفند بلوچی با استفاده از اطلاعات نشانگرهای SNP موجود در سراسر ژنوم حاصل از تراشه متراکم است. با استفاده از نرم افزار NEESTIMATOR، اندازه مؤثر کنونی جمعیت براساس روش های هتروزیگوسیتی اضافی و LD به ترتیب 15 و 25 تخمین زده شدند. نتایج به دست آمده از اندازه مؤثر تاریخی جمعیت با استفاده از نرمافزار SNeP، نشان دهنده یک روند کاهشی سریع در طول 1000 نسل گذشته بود. اندازه Ne در چهار نسل قبل در دامنه 75-48 رأس بود. ضریب همخونی با استفاده از سه روش ماتریس روابط خویشاوندی (FGRM)، میزان هموزیگوسیتی (FHOM)و همبستگیگامتها (FUNI)با استفاده از نرمافزارGCTA محاسبه شدند. مقادیر FGRM، FHOM وFUNI بهطور مشابه برابر با 0.017- محاسبه شدند. میانگین هتروزیگوسیتی مورد انتظار و مشاهده شده با استفاده از نرمافزارPLINK به ترتیب 0.374 و 0.383 برآورد شدند. نتایج این تحقیق نشان داد که تنوع ژنتیکی مناسبی در نژاد بلوچی وجود دارد اما مقادیر پایین Ne بیانگر اجرای اقدامات ویژه ای برای جلوگیری از کاهش شدیدتر اندازه مؤثر جمعیت در این نژاد است. بنابراین طراحی برنامه های مناسب برای حفاظت از حیوانات خالص این نژاد ضروری است.
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