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Open Access Article
1 - A survey of prevalence and antibiotic resistance pattern of Staphylococcus aureus strains isolated from eggs in Ardabil area, Iran
آیدین Azizpour,Staphylococcus aureus is one of the main causes of foodborne diseases globally. Eggs can contaminated with S.aureus when they make contact with dirty surfaces, litter materials as well as clothing and hands of poultry workers. Therefore, the purpose of this study was to MoreStaphylococcus aureus is one of the main causes of foodborne diseases globally. Eggs can contaminated with S.aureus when they make contact with dirty surfaces, litter materials as well as clothing and hands of poultry workers. Therefore, the purpose of this study was to determine the prevalence and antibiotic resistance of S. aureus strains isolated from eggs in Ardabil area located in the northwest of Iran. One hundred and fifty eggs were collected randomly from poultry farms (n=50) and retail outlets (n=50 native and n=50 bulk) in different parts of Ardabil. In terms of S. aureus contamination, eggs were examined using bacterial culture and biochemical tests. From 150 egg samples examined, 9 samples (9.3%) were infected with S. aureus. Out of these, the highest contamination rate of S. aureus was observed in native, industrial and bulk eggs 50%, 28.58% and 21.42%, respectively. There was the highest resistance to penicillin (78.57%), tetracycline (71.42%) and ampicillin (64.28%), while the lowest resistance was observed to chloramphenicol (21.42%), gentamicin (14.28%) and ciprofloxacin (7.14%). All the S. aureus isolates were susceptible to vancomycin (100%). The results of this study indicate the presence of S. aureus infection in eggs and the relatively high prevalence of S. aureus isolates resistant to some antibiotics is a potential risk to human health. It is necessary to pay attention to hygienic points in storing and consuming eggs and monitoring the use of antibiotics. Manuscript profile -
Open Access Article
2 - Incresing Shelf Life of of Penaeus semisulcatus in NanoSilver Coatings Based on Titanium Dioxide
H. Ahari Z. Amanolah nejad M.A. Magharehei S. PaidariIntroduction: Shrimp is one the most famous food with high nutritional value. Shrimp is undoubtedly one of the best-known sea foods and determination of appropriate methods for its packaging increases the shelf life of products and reduces the microbial load in all food MoreIntroduction: Shrimp is one the most famous food with high nutritional value. Shrimp is undoubtedly one of the best-known sea foods and determination of appropriate methods for its packaging increases the shelf life of products and reduces the microbial load in all food processing plants. Materials and Methods: The aim of this study is to implement nano packaging to evaluate its antibacterial characteristics, shelf life determination as well as evolution of quality changes during shelf life. The current study evaluates the antibacterial features of produced nanopackaging containing 1000 to 6000 ppm of nanosilver during 0,3,7,10,14,21 and 25 days concerned will total count, E.coli and S.aureus, sensory analysis and determination of size and distribution of silver nano particles using SEM and FESEM. Results: The results showed that there is a significant difference between the control and produced nanopackaging. Moreover, there were a significant difference between the antibacterial effects of nanopackagings on E.coli and S.aureus (P<0.05). Conclusion: Sensory analysis of the product revealed that there is no significant difference between sensorya and quality characterristics of nano-packed shrimps and control shrimps. Manuscript profile -
Open Access Article
3 - Screening of Antagonistic Activity of Probiotic Bacteria Against Some Food-Borne Pathogens
M. El-Kholy S.H. El-Shinawy A. M. S. Meshref A. M. Korny -
Open Access Article
4 - Cytotoxicity, Anti-microbial activity and molecular docking simulation of novel Bis-chalcones linked to tetrahydro-[1,2,4]triazolo[3,4-a] isoquinoline moiety
Nada S. Ibrahim Elshimaa M. Eid Huwaida M. E. Hassaneen Hamdi M Hassaneen Ahmed H. M Elwahy Ismail A Abdelhamid -
Open Access Article
5 - Self-assembly of ZnO nanoparticles on Low-Density Polyethylene Film with sol- gel and its Application for Milk Active Packaging
Mohammad Ali Shayegh sayedehmona Alavinassab Somayeh Shahedi Saeid JafariThis study reports the antibacterial capability of low-density polyethylene (LDPE) modified with ZnO nanoparticles using a Sol-Gel technique. Antibacterial activity of prepared films against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) was examined. MoreThis study reports the antibacterial capability of low-density polyethylene (LDPE) modified with ZnO nanoparticles using a Sol-Gel technique. Antibacterial activity of prepared films against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) was examined. The operational conditions such as pH, time, amount of ZnO nanoparticles, and silanol concentration were optimized using the response surface methodology (RSM). The proposed film under optimum conditions was applied for the packaging of the milk sample. The highest antibacterial activity of ZnO/LPDE were pH (6.0), time (103 min), amount of ZnO nanoparticles (0.68 % w/v) and silanol agent concentration (4.81 % v/v). The antibacterial properties of ZnO/LDPE films were assessed based on the diameter of the inhibition zone in a disk diffusion test against E. coli and S. aureus. These films have significantly reduced the growth of mentioned bacteria. Overall, antimicrobial packaging shows promise as an effective method to inhibit the growth of certain bacteria like E. coli and S. aureus in milk. The resulting ZnO/LDPE package films containing milk samples exhibit superior and prolonged antibacterial activity against E. coli and S. aureus in 7 and 14 days. Manuscript profile -
Open Access Article
6 - Profile of erythromycin resistance genes ermA, B, C and msrA in Staphylococcus aureus strains isolated from Ahvaz hospitals
Zahra Noormohammadi Zahra Noormohammadi Shohre Zare Karizi sahar honarmand jahromiStaphylococcus aureus is currently one of the main causes of nosocomial. The present study aimed to evaluate erythromycin resistant genes in Staphylococcus aureus strains isolated from clinical samples obtained from hospitals. In this study, 100 patients with positive S MoreStaphylococcus aureus is currently one of the main causes of nosocomial. The present study aimed to evaluate erythromycin resistant genes in Staphylococcus aureus strains isolated from clinical samples obtained from hospitals. In this study, 100 patients with positive S. aureus in clinical samples were collected from hospitals in Ahvaz. Resistance to antibiotics erythromycin, tetracycline, penicillin and clindamycin using antibiogram disks on all samples of blood, urine, sputum and trachea was tested. The presence of four genes involved in antibiotic resistance, namely ermA, ermB, ermC and msrA were determined by PCR in genome and plasmid. Also 21 samples with positive ermC gene were sequenced for evaluation of genetic variation. Molecular analysis showed that 64 samples were resistant to erythromycin while 87.5%, 93.8%, 92.2% and 70.3% of samples were positive in ermA, ermB, ermC and msrA respectively and 25 samples were susceptible to erythromycin including % 88 ermA, 92 % ermB, 100% ermC and 48% msrA with P value of: 0.23, 0.66, 0.31 and 0.83 showed no significant differences between susceptible and resistance to erythromycin samples. The presence of erm genes in erythromycin susceptible samples may be due to mutations in promoters or coding regions of genes which may inhibit their functions. Also the results of sequencing of ermC gene proved presence of this gene on plasmids. Manuscript profile -
Open Access Article
7 - Effect of silver nanoparticles on surface hydrophobicity and biofilm formation in Staphylococcus aureus, Acinetobacter baumannii, Pseudomonas aeruginosa and Escherichia coli
alireza Farazandeh Fatemeh Noorbakhsh Sahar Honarmand JahromiIntroduction: Biofilms are microbial aggregates that adhere to a substrate as a surface and are surrounded by an exopolysaccharide matrix. The bacteria that make up biofilms are so resistant to antimicrobials and antibiotics that this has led to concerns in the medical MoreIntroduction: Biofilms are microbial aggregates that adhere to a substrate as a surface and are surrounded by an exopolysaccharide matrix. The bacteria that make up biofilms are so resistant to antimicrobials and antibiotics that this has led to concerns in the medical community.The aim of this study was effect of silver nanoparticles on surface hydrophobicity and biofilm formation in Staphylococcus aureus, Acinetobacter baumannii, Pseudomonas aeruginosa and Escherichia coli. Methods: This study was performed on 40 bacterial strains including Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli and Acinetobacter baumannii isolated from Milad Hospital in Tehran. The strains were identified by biochemical tests. Also, biofilm formation by microtiter plate method, surface hydrophobicity by MATH method and sensitivity of strains to silver nanoparticles were evaluated.Results: According to the results obtained in this study, all strains were able to form biofilms and no strains with negative biofilms were observed in the study. The highest effect of silver nanoparticles in this study was observed on strains at concentrations of 0.5, 1 and 2 μg / ml. Also, only 20% of Pseudomonas aeruginosa strains in this study were observed with moderate hydrophobicity and the others of the bacteria were weakly hydrophobic.Conclusion: silver nanoparticles in low concentrations with the effect of low toxicity had the ability to inhibit the biofilm formation of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Acinetobacter baumannii. Hydrophobicity in the studied bacteria were weak. Manuscript profile -
Open Access Article
8 - Antibacterial effects of green laser in vicinity of silver nanoparticles on methicillin-resistant staphylococcus aureus (MRSA)
Maliheh Ranjbaran Zahra Aghaebrahimi Jamshid Sabaghzadeh zohreh daraeizadeh arash abednezhad -
Open Access Article
9 - Grape Seed Extract Loaded Amino Functionalized SBA-15 Mesoporous Silica Nanoparticles as a potential drug delivery System: Antibacterial activity and release kinetics studies
Mahdi Shahriarinour Faten Divsar Zahra Nazari ShadMesoporous silica nanoparticles (MSNs) are used as efficient carrier candidate for the delivery of bioactive molecules, proteins, and drugs because of their properties such as safety, stability, biocompatibility, high surface area, and controllable pore sizes with narro MoreMesoporous silica nanoparticles (MSNs) are used as efficient carrier candidate for the delivery of bioactive molecules, proteins, and drugs because of their properties such as safety, stability, biocompatibility, high surface area, and controllable pore sizes with narrow distribution and facile surface modification. MSNs demonstrate high encapsulation capability for both hydrophilic and hydrophobic drugs. In this study, SBA-15 MSN was synthesized by hydrothermal method in acidic media and functionalized by post-grafting method using 3-aminopropyltriethoxysilane. The efficiency of amino-functionalized SBA-15 MSN (SBA-15-NH2) was investigated for the loading and release of grape seed extract (GSE). The disc diffusion assay indicated GSE and GSE loaded on SBA-15-NH2 (SBA-15-NH2@GSE) at the concentration of 32 mg/ml extract per disc resulted in the inhibition zone of 24 mm and 15 mm for S. aurous and 22 mm and 13 mm for E. coli, respectively. The antibacterial effect improved by increasing the concentration of GSE loaded into SBA-15-NH2 from 2 to 32 mg/ml. These results indicate that SBA-15-NH2@GSE owns a strong antibacterial potential valuable for the pharmaceutical industry. Manuscript profile -
Open Access Article
10 - Effect of nisin on microbial quality of farmed great sturgeon Huso huso in refrigerator and freezing temperatures
محمدرضا قمی مسعود Ghane مینا Eghbali مهدی NikooEffect of nisin on microbial quality of farmed great sturgeon Huso huso in refrigerator and freezing temperatures In this study the effects of nisin (a biotic preservative) (0, 0.2 and 0.4%) on the microbiological quality of farmed great sturgeon (H. huso) slices inocu MoreEffect of nisin on microbial quality of farmed great sturgeon Huso huso in refrigerator and freezing temperatures In this study the effects of nisin (a biotic preservative) (0, 0.2 and 0.4%) on the microbiological quality of farmed great sturgeon (H. huso) slices inoculated by Staphylococcus aureus and Escherichia coli (8 log cfu/ml) during storage (4 and -18 °C) for 10 days was determined. The counts of S. aureus did not change significantly after ten days of storage (P> 0.05) and in other words, it was not affected from different concentrations of nisin and storage temperatures. Frozen samples treated by nisin showed lower counts of E. coli. Nisin at both concentrations had the same effects on reducing the counts of both bacteria. The positive effects of such a low-risk biological preservative in studied food product appearing to reduce the microbial load, therefor its usage in preservation of food is industrially preferable rather than other chemical preservatives. Keywords: Nisin, Storage temperature, Staphylococcus aureus, Escherichia coli, Farmed beluga. Manuscript profile -
Open Access Article
11 - Effect of Purified Bacteriocin from Acinetobacter baumannii on some Pathogenic and Environmental Isolates and Its Inhibitory Effect on Hemolysin Production from S. aureu
Sahar Jabar Nasser Raghad A. Abdulrazaq