List of Articles Mycoplasma gallisepticum

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  1 - Isolation and identification of Mycoplasma gallisepticum from commercial broiler Flocks in Ghaemshahr Town ship
  امید طیبی درازکلا سیدعلی پوربخش منصور بنانی پیمان حجتی زهرا صلاحی مه زاد ارمی
  Mycoplasmosis a common disease in the poultry industry. Among the most important illnessmakingin birds Mycoplasma, it can be called Mycoplasma gallisepticum. But so far, we did nothave any research about finding Mycoplasma gallisepticum by using of PCR method in Ghaemsh More

  Mycoplasmosis a common disease in the poultry industry. Among the most important illnessmakingin birds Mycoplasma, it can be called Mycoplasma gallisepticum. But so far, we did nothave any research about finding Mycoplasma gallisepticum by using of PCR method in GhaemshahrTown ship. The goal of this study (research) was isolation and identification Mycoplasmagallisepticum of Ghaemshahr Town ships commercial broiler flocks with using from microbiologyand molecules (PCR) methods. In this research performed sampling from eighty one ofGhaemshahr Town ships commercial broiler flocks. at the beginning of this work , from commercialbroiler flocks performed biopsy of windpipe, sirenx and airsocks and after transmitting samples toRazi institute, it began to culture PPLO bruth place and agar into sampling of tissues. in thisresearch, from the whole eighty one sampled farm broiler, twenty farm broiler in regard to culture,and forty farm broiler in PCR experiment in regard to genus be positive as well as ten farm broilerin PCR experiment in regard to Mycoplasma gallisepticum species. The results indicate thatGhaemshahr Town ships farm broiler be contaminated to Mycoplasma gallisepticum and basicprogramme is necessary for control and prophylaxis disease s factor, and be recommended tomaking use of molecule s experiment such as PCR for identification diseases factor and immediatediagnosis. Manuscript profile
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  2 - Detection of Mycoplasma gallisepticum by 16S rRNA PCR with specific primers in clinical samples
  S.A Pourbakhsh افشین Zakeri نریمان Sheikhi سعید Charkhkar عباس Ashtari
  Mycoplasma gallisepticum (MG) as one the major pathogens of birds, causes significant economic losses in poultry industry. The main purpose of the present study was to detect Mycoplasma gallisepticum in clinical samples using the 16S rRNA PCR method. For serological scr More

  Mycoplasma gallisepticum (MG) as one the major pathogens of birds, causes significant economic losses in poultry industry. The main purpose of the present study was to detect Mycoplasma gallisepticum in clinical samples using the 16S rRNA PCR method. For serological screaming test, 18 commercial laying farms and 8 broiler breeder farms were selected and rapid serum agglutination test (RSAT) was performed. For polymerase chain reaction sampling, 10 of the 17 farms that were positive in RSAT were selected and 109 sterile swab samples were collected from the palatine cleft, trachea, air sacs and lungs in each farm. Three swabs from three birds were placed in test tube containing 1 ml of phosphate buffered saline and transferred to laboratory form PCR testing. In this study, specific primers for 16S rRNA gene were used. The aforementioned primers are totally specific for MG and can be differentiated from other Mycoplasmaand bacteria present in the trachea of poultry of the 26 farms examined, 17 farms were positive in RSAT serologic test. The 530 bp PCR product produced by specific primers of all field strains appeared on electrophoresis gel in 46 samples from 10 farms accounting to 42.2%.The 16S rRNA PCR with very high sensitivity can be employed in definitive diagnosis of Mycoplasma gallisepticum infection in vitro.   Manuscript profile
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  3 - واکنش زنجیره پلیمراز ژن های Mgc2 و 16S rRNA جهت تشخیص مایکوپلاسما گالی‪سپتیکوم
  ا. ذاکری
  مایکوپلاسما‪ها باکتری‪های بسیار کوچکی هستند که فاقد دیواره سلولی و دارای جنس‪های مختلف که در قالب کلاس مولیکاتوس قرار می‪گیرند. این باکتری به عنوان کوچکترین میکرو ارگانیسمی است که به طور مستقل می‪تواند زندگی نماید. این دسته از باکتری‪ها می‪توانند بیماری‪های جدی و مزمن ا More

  مایکوپلاسما‪ها باکتری‪های بسیار کوچکی هستند که فاقد دیواره سلولی و دارای جنس‪های مختلف که در قالب کلاس مولیکاتوس قرار می‪گیرند. این باکتری به عنوان کوچکترین میکرو ارگانیسمی است که به طور مستقل می‪تواند زندگی نماید. این دسته از باکتری‪ها می‪توانند بیماری‪های جدی و مزمن ایجاد نمایند. مایکوپلاسما گالی‪سپتیکوم به عنوان یکی از مهمترین عوامل میکروبی پاتوژن پرندگان محسوب می‪گردد که باعث خسارات اقتصادی فراوان در صنعت طیور می‪گردد. هدف از انجام این مطالعه، بررسی وجود مایکوپلاسما گالی‪سپتیکوم در طیور به وسیله واکنش زنجیره پلی‪مراز ژن‪های mgc2 و 16S rRNA بود. برای این کار پرایمرهای اختصاصی برای ژن‪های مذکور مورد استفاده قرار گرفت. در ابتدا جهت غربالگری از تست سرمی RSAT از 26 فارم پرورشی نمونه خون اخذ گردید. هچنین جهت نمونه برداری برای PCR، 109 نمونه از نمونه‪هایی که در آزمایش RSAT مثبت شده بودند، اخذ گردید که این نمونه‪ها شامل سواب‪هایی از ریه، کیسه‪های هوایی و نای را شامل می‪شد. با توجه به اختصاصی بودن پرایمرهای استفاده شده در این مطالعه، در آزمایش PCR، باند 530 جفت بازی برای ژن 16S rRNA و باند 300 جفت بازی برای ژن mgc2 در ژل الکتروفورز تشکیل گردید که وجود مایکوپلاسما گالی‪سپتیکوم در این نمونه‪ها را تأیید می‪نمود. این تست می‪تواند به صورت معمول در آزمایشگاه‪ها جهت تشخیص باکتری مایکوپلاسما گالی‪سپتیکوم در کوتاهترین زمان ممکن و مستقیماً بر روی نمونه‪های بالینی انجام گیرد. Manuscript profile
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  4 - In silico analysis of pMGA1.2 protein of Mycoplasma gallisepticum in vaccine and pathogenic strains
  Farzaneh Pourkarimi Fatideh Majid Esmaelizad Mohammad Kargar Majid Tebianian Farshid Kafilzadeh
  10.30495/jmw.2023.1982503.2058
  Background & Objectives: Mycoplasma gallisepticum, the pathogen responsible for chronic respiratory disease in chickens, is the most economically important species of Mycoplasma that causing tremendous economic losses worldwide. The most abundant membrane proteins i More

  Background & Objectives: Mycoplasma gallisepticum, the pathogen responsible for chronic respiratory disease in chickens, is the most economically important species of Mycoplasma that causing tremendous economic losses worldwide. The most abundant membrane proteins in M. gallisepticum are pMGA, lipoproteins of about 67 kDa. The pMGA family genes have an extraordinary potential for diversifying antigenic structure on the surface of Mycoplasma gallisepticum cells. The aim of this study was to compare the pMGA protein patterns between different strains and hosts of Mycoplasma gallisepticum. Material & Methods: All Mycoplasma gallisepticum full genomes available in GenBank till January 2020 were considered and pMGA1.2 sequences were identified, grouped and coded. pMGA1.2 protein with a chain of 650 amino acids between two different hosts (poultry and house finch) was studied by bioinformatics software in all Mycoplasma gallisepticum full genomes. Results: pMGA1.2 gene among different strains of Mycoplasma gallisepticum showed five major groups with more than 10 percent divergence. Based on multiple sequence alignment, a specific pattern was identified in house finch isolates. Interestingly, two specific motifs 480DNQNVSNQ487 and 639SSNVSSPSY647 were found in the pMGA1.2 of TS-11 strain, which can probably be used as markers to identify and differentiate this vaccine strain from pathogenic Mycoplasma gallisepticum. Conclusion: This study showed that pMGA1.2 protein have some B-cell epitope antigenic regions that are conserved among all isolates and might be applicable to design serological test for detection antibody against Mycoplasma gallisepticum. Manuscript profile
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  5 - Seroprevalence of Mycoplasma gallisepticum infection in commercial layer farms in Alborz and Qazvin provinces
  پیام Haghighi Khoshkhoo, گیتا Akbari-Azad, مسعود Roohi, پدرام Sami-Yousef,
  Mycoplasma gallisepticum is associated with signifcant economic losses in domestic poultry, caused suboptimalegg production in layers. This survey was carried out to determine the seroprevalence of Mycoplasma gallisepticum infection in commercial layer farms in Alborz a More

  Mycoplasma gallisepticum is associated with signifcant economic losses in domestic poultry, caused suboptimalegg production in layers. This survey was carried out to determine the seroprevalence of Mycoplasma gallisepticum infection in commercial layer farms in Alborz and Qazvin provinces for primary evaluation of usage of liveMg vaccine in commercial layer flocks. A total of 2050 serum samples were collected from 41 commercial layerflocks (50 samples from each farm) mostly over 40-week-old. Sera were tested by Serum Plate Agglutination(SPA) method using commercial Mg antigen (Noblis MG®, Intervet Co, Holland). Positive reactions retested bySPA on 1:8 dilution and the farms with more than 10% positive reactions were considered positive serologically.The result showed that in the Alborz province, only one out of 21 (4.8%) farms were positive and the rest (95.2%)were negative; also, 51 out of 1050 (4.86% ) sera were positive, 991 out of 1050 (94.38%) were negative and 8out of 1050 (0.76%) were doubtful. In Qazvin province, 3 out of 20 (15%) farms were positive and 17 out of 20(85%) were negative; also, 74 out of 1000 (7.4%) sera were positive, 46 out of 1000 (4.6%) were doubtful and880 out of 1000 (88%) were negative. As seroprevalence of Mg infection in these two provinces which are veryimportant areas in poultry production in Iran were low,so it seems that using live vaccine against Mg needs morestudies. However, molecular identifcation should be used for completing this fnding and biosecurity notices arevery important for controlling of Mg. Manuscript profile