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      • Open Access Article

        1 - Contact toxicity of three plant essential oils on adult insects of Sithophilus oryzae (Coleoptera: Curculionidae)
        Yasamin Motamedi Majid Fallahzadeh Vahid Roshan
        Recently, there has been growing interest in research concerning the possible use of plant extracts as alternatives to synthetic insecticides. Essential oils are among the best-known substances tested against insects. These compounds may act as fumigants, contact insect More
        Recently, there has been growing interest in research concerning the possible use of plant extracts as alternatives to synthetic insecticides. Essential oils are among the best-known substances tested against insects. These compounds may act as fumigants, contact insecticides, repllants, antifeedants and may affect growth and development of the insect. In an attempt to find a natural and inexpensive method for the control of stored-product pests, contact toxicity of essential oils of Mentha longifolia L., Tagetes minuta L. and Salvia macrosiphon Boiss. was investigated on adult insects of Sitophilus oryzae L. (Coleoptera, Curculionidae) on laboratory condition. The oils were extracted by using Clevenger apparatus. The experiment was conducted using a completely randomized design of factorial experiment with five concentrations and three replications at R.H in darkness. The results showed that the percentage of mortality was increased with increase in concentration and exposure of time. The highest contact toxicity was related to M. longifolia and the lowest toxicity was recorded for S. macrosiphon against insects. At the highest concentration of essential oils (35 µl/ml) by M. longifolia,T. minuta and S. macrosiphon the mortality were recorded as 93.3%,83.3% and 70% against S. oryzae after 48h, respectively. The LC50 values were evaluated by M. longifolia, T. minuta and S. macrosiphon 18.892, 21.003 and 24.482 for S. oryzae after 48h .According to the results, the essential oils of M. longifolia,T. minuta and S. macrosiphon are sources of biologically active vapor that can be used in protection of stored grains against  rice weevil. Manuscript profile
      • Open Access Article

        2 - Fumigant toxicity of essential oils extracted from three plant species against Sithophilus oryzae (L.) (Coleoptera: Curculionidae)
        Y. Motamedi M. Fallahzadeh V. Roshan
        Over the past few decades, many investigations have been conducted on different plant products in order to obtain safer and more effective alternatives rather than chemical insecticides for controlling store-product insects. For this purpose, in search of alternatives a More
        Over the past few decades, many investigations have been conducted on different plant products in order to obtain safer and more effective alternatives rather than chemical insecticides for controlling store-product insects. For this purpose, in search of alternatives agents for conventional pesticides, the fumigant activity of essential oils from Mentha longifolia L., Tagetes minuta L. and Salvia macrosiphon Boiss. were tested against adult insects of Sitophilus oryzae L. (Coleoptera: Curculionidae) on laboratory condition. The experiment was conducted using a completely randomized design of factorial experiment with five concentrations and three replications at 25±10C, 60±5% R.H in darkness. The results showed that the percentage of mortality was increased with increase in concentration and exposure period. The highest fumigant toxicity was related to M. longifolia and the lowest toxicity was recorded for S. macrosiphon against insects. At the highest concentration of essential oils (25μl/L of air) by M. longifolia, T. minuta and S. macrosiphon the mortality were recorded as 80%, 73.3% and 66.6% after 12h and 96.6%, 86.6% and 73.3% after 24h against S. oryzae, respectively. The LC50 values were evaluated by M. longifoli, T. minuta and S. macrosiphon 8.166, 11.488 and 14.161 μl/L of air after 12h and 5.898, 7.814 and 11.068 μl/L of air after 24h for S. oryzae. According to the results, the essential oils of M. longifolia, T. minuta and S. macrosiphon are source of biologically active vapor that can be used in protection of stored grains against rice weevil. Manuscript profile
      • Open Access Article

        3 - Study on the chemical composition and insecticidal activity of the wild mint (Mentha longifolia L.) essential oil against the melon aphid (Aphis gossypii Glover)
        M. Zamani verdi H. Abbasipour S. Goudarzvande Chegini
        The melon aphid, Aphis gossypii Glover is a polyphagous and cosmopolite species which is widely distributed in moderate and tropical regions of the world. The high-risk chemical pesticides are currently used to control this pest that have environmental impacts and also More
        The melon aphid, Aphis gossypii Glover is a polyphagous and cosmopolite species which is widely distributed in moderate and tropical regions of the world. The high-risk chemical pesticides are currently used to control this pest that have environmental impacts and also affect non-target organisms. Therefore, in order to cope with this situation, we must think about the safe alternatives to control this pest. In the current study, chemical composition and fumigant toxicity of the essential oil from wild mint, Mentha longifoliaL. was studied against the nymphal and adult stages of the melon aphid, A. gossypii. The essential oil was obtained by hydro-distillation method, using a modified Clevenger-type apparatus. The amount of this essential oil was determined by mass chromatography method. Bioassay experiments were performed in a completely randomized design with five concentrations (0.1 to 0.9 μl/L air) and three replications. For each replicate, 10 samples of the last instar nymphs and adult aphids were separately considered. Also, nymph production deterrent effect of the oil at sublethal concentration of the oil was studied against the parthenogenesis form of aphid. Results indicated that essential oil of M. longifolia is highly toxic to the melon aphid.The major components in the oil were Pulegone (44.75%), Menthone (30.80%), 1,8- Cineole1 (5.49%) and Cyclohexanone, 5-methyl-2-(1-methylethyl)- (2R-cis (2.06%). Probit analysis showed that the LC50 values for nymphs and adults of A. gossypii were 0.059 and 0.0081 µl. L-1 air, respectively. By increasing the concentration of essential oil, the mortality rate also increased. Also, degree of nymph production deterrent effect was calculated for the essential oil of M. longifoliaas 80.03±3.68%. The overall results showed that the wild mint essential oil has a high potential in controlling the melon aphid especially in protected areas such as geenhouses.   Manuscript profile
      • Open Access Article

        4 - Fumigation toxicity of four plant essential oils on adults of Tribolium castaneum (Herbst) and T. confosum ( Du val)
        S. Mahmoodvand J. Shakarami R. Vafaei-Shoushtari
        The effects of four plant essential oils of Mentha longifolia, Thymus deanensis, Achillea wilhelmsii and Artemisia hausskenechtii were investigated on adult stage of Tribolium castaneum and T. confusum. The essential oils were prepared using water distillation method. E More
        The effects of four plant essential oils of Mentha longifolia, Thymus deanensis, Achillea wilhelmsii and Artemisia hausskenechtii were investigated on adult stage of Tribolium castaneum and T. confusum. The essential oils were prepared using water distillation method. Essential oils were used in five concentrations and five replications. The concentration selected based on primary experiment for each essential oil. Mortality rate of insects were recorded after 3, 6, 24, 48 and 72 hours. Results showed that the longer exposure of insect to essential oil and the higher concentration of essential oil, increased  mortality of the two beetle species in all treatments. There was significant difference among mortality effect of the essential oils. The LC50 value of M.longifolia after 24 hours for T. castaneum and T. confosum were 11.59 and 10.67 µl/lair respectively, while the values of LC50were 89.21 and 99.79 for T. daenensis, 55.40 and 98.71 for A. wilhelmsii and 60.64 and 62.25 µl/lair for A. hausskenechtii, respectively. The least LT50 recorded was in A. hausskenechtii essential oil with 23.10 and 21.10 hours against T. castaneum and T. confusun, respectively. Results showed that these essential oils can be as safe botanical pesticides for control of storage pests. Manuscript profile
      • Open Access Article

        5 - Effect of edible carrageenan- Mentha longifolia essential oil coating on microbial, chemical and sensory characteristics of fresh chicken fillet during refrigeration storage
        S. Kiani Ghalesard A. Fazlara M. Ghaderi-Ghahfarokhi M. Pourmahdi
        The coating is one of the methods to extend the chicken fillet shelf life. The present study was conducted to evaluate the effect of carrageenan edible coating (1%) containing (Mentha longifolia) essential oil (1.5%) on the quality of chicken fillet during refrigerated More
        The coating is one of the methods to extend the chicken fillet shelf life. The present study was conducted to evaluate the effect of carrageenan edible coating (1%) containing (Mentha longifolia) essential oil (1.5%) on the quality of chicken fillet during refrigerated storage. Samples were separated into three groups: uncoated (control), coated with carrageenan and coated with carrageenan contained essential oil (Mentha longifolia) were stored at refrigerator temperature for 21 days and were evaluated for microbial (psychrotrophic and mesophilic) counts, chemical properties [pH, total volatile nitrogen (TVN) and thiobarbituric acid (TBA)] and sensory characteristics (appearance, muscles elasticity, odor, and color). The results of the bacterial analysis showed that coating with carrageenan and carrageenan- essential oil coatings had significant effects on delaying the increasing trend of psychrophilic and mesophilic bacterial counts as compared to control. Chemically, carrageenan-essential oil treatment showed lower TBA, TVN, and pH values ​​than the other two groups during storage (P< 0.01). Moreover, the carrageenan and carrageenan contained essential oil treatments maintained sensorial factors at acceptable levels for 3 and 9 days respectively. Based on the results of the present study, carrageenan coating did not have the ability to extend the shelf life of chicken fillets but, the carrageenan-essential oil coating could extend the shelf life of chicken fillets for 9 days. Manuscript profile
      • Open Access Article

        6 - Investigating the variation of essential oil content, chlorophyll, carotenoid, anthocyanin and flavonoid of (Mentha longifolia ( L.) Hods. subsp. Longifolia) in several habitats of Marand
        Vahid Noroozi Saeed Yousefzadeh Kama lSadat Asilan Sirius Mansourifar
        In this research to determine the qualitative phytochemical characteristics of Mentha longifolia (L.) Hods. subsp. longifolia populations  in different  habitats , an experiment was conducted as completely randomized design with three replications during 2015. More
        In this research to determine the qualitative phytochemical characteristics of Mentha longifolia (L.) Hods. subsp. longifolia populations  in different  habitats , an experiment was conducted as completely randomized design with three replications during 2015. Aerial parts of plants were collected in different habitats of plant (Farfar, Yekan-Sadi, Bengin, Koshksara and Darvishmohammad - 1100 to 1300 m) in East Azerbaijan province and the plant samples removed under ground in 0.5×0.5 quadrate. Essential oils were extracted by hydro distillation method. The phytochemical analyses: total phenol, flavonoide, anthocyanine, caretenoide and chlorophyll were measured by using spectrophotometric technique. The results were showed that the amount of essential oil yield, total flavonoids, phenol, chlorophyll, carotenoid and anthocyanin content had high variation in different regions, but in Bengin population the plant had the highest content of these metabolites, respectively. In conclusion showed that the Bengin region had high altitude, with the highest nitrogen and organic matter content of soil and there was the positive correlation between the increasing of essential oil and another secondary metabolites of plant with the photosynthetic pigments contents. So due to high levels of plant metabolites compounds in Bengin population, this population of plant in this region can be produced cultivars with desirable crop traits. Manuscript profile
      • Open Access Article

        7 - Essential oil composition of Mentha longifolia L. Hudson. in different parts of Fars and Khorasan Razavi provinces
        Hassan Feizi zohre hoseeini Safiyeh Vatandoost Jertoodeh masoud alipanah
        Mentha longifolia (L.) Hudson. is belongs to Lamiaceae family which has been used as antiseptic, anti inflammation , astringent , tonic, antispasmodic, carminative and calming. The current experiment was aimed to comparision of quality and quantities of plant essential More
        Mentha longifolia (L.) Hudson. is belongs to Lamiaceae family which has been used as antiseptic, anti inflammation , astringent , tonic, antispasmodic, carminative and calming. The current experiment was aimed to comparision of quality and quantities of plant essential oil  in different parts of Fars and Khorasan Razavi provinces. The aerial parts of plant in blooming were collected from ten natural regions in two provinces during summer 2015 . The essential oils were extracted by  using water distillation method and  were analyzed by  GC and GC/MS. Results were showed that the highest essence efficiency of M. longifolia were reported from Chenaran, Mashhad and Kashmar regions  in Khorasan province. The highest of 1,8-cineole (36.47%) and mentole (16.3%) were reported from Fasa region, but pulegon with 45,2-49.5% were obtained from Kuvar and Bavanat region, respectively and finaly the highest of piperiton oxide (18.3%) and piperitenone (55.3%) were reported from Kazerun region in Fars province. So in conclusion, the M.longifolia due to the presence of more variation multiple compounds have significant potential for use in domestication and breeding programs.   Manuscript profile
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        8 - مقایسه اثر ضد باکتریایی عصاره اتانولی برگ گیاه Mentha longifolia (L.) Hud. در رویشگاههای مختلف شمال ایران
        پریسا تربتی نژاد سیده معصومه میرتقی فصیحه لیوانی هانیه باقری
      • Open Access Article

        9 - The antimicrobial effects of Mentha longifolia L. essential oil on Salmonella enteritidis in mayonnaise sauce
        shaghayegh Ghorbani Leila Rouzbeh-nasiraei Mohammad Hassan Juri
        Now adays, there is a considerable tendency to use herbal essential oil as natural preservatives in foods and nutrition. Studies of their antimicrobial impact in the food models to achieve a safe and healthy food is growing. Mentha longifolia plant of the Laminacea fami More
        Now adays, there is a considerable tendency to use herbal essential oil as natural preservatives in foods and nutrition. Studies of their antimicrobial impact in the food models to achieve a safe and healthy food is growing. Mentha longifolia plant of the Laminacea family has antimicrobial compounds, Carvacrol and Thymol which are found in the essential oil of this plant. The aim of this study is to survey and evaluate the antimicrobial activity of essential oils of Mentha longifolia and the possibility of its application as a natural preservative and flavor in mayonnaise. In this study, the antimicrobial effect of different concentrations (1250, 2500, 5000 and 10000 ppm) Mentha longifolia essential oil on Salmonella enteritidis in mayonnaise at 4 and 25 °C were evaluated in 30 days. Factorial experiments in a completely randomized design, with three repetitions were analyzed by SPSS software. The results showed that the antimicrobial effect of “essential oil of oregano” at 25 °C is higher than 4 °C (p<0.05) and this effect increased by increasing the density of oil in the samples. The greatest impact of the essential oil concentration was 10000 ppm at both temperatures. While the increase in essential oil concentration in the samples, the sensory acceptability of food was reduced considerably. According to the results of sensory evaluation and microbial tests, the concentration of 1250 ppm oil in mayonnaise samples, have favorable results in reducing the number of bacteria up to 6 logarithms and gain a sensory of acceptance. Manuscript profile
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        10 - اثر اسانس چند گیاه دارویی بر سیستم ایمنی ماهی قزل آلای رنگین کمان Oncorhynchus mykiss
        عبدالله قاسمی پیربلوطی اسماعیل پیرعلی غلام رضا پیشکار سید محمدعلی جلالی مهدی رئیسی محسن جعفریان دهکردی بهزاد حامدی
        مقدمه و هدف: اهمیت ماهی قزل آلا رنگین کمان (Oncorhynchus mykiss) در صنعت آبزی پروری ایران از یک سو و افزایش تلفات ناشی از تنش های مختلف مانند شیوع بیماری های عفونی و عدم تأثیر داروهای سنتزی از سوی دیگر، ما را بر آن داشت تا تحقیق حاضر را به منظور بهبود سیستم ایمنی و کاهش More
        مقدمه و هدف: اهمیت ماهی قزل آلا رنگین کمان (Oncorhynchus mykiss) در صنعت آبزی پروری ایران از یک سو و افزایش تلفات ناشی از تنش های مختلف مانند شیوع بیماری های عفونی و عدم تأثیر داروهای سنتزی از سوی دیگر، ما را بر آن داشت تا تحقیق حاضر را به منظور بهبود سیستم ایمنی و کاهش تلفات این ماهی با استفاده از ترکیبات طبیعی مانند اسانس گیاهان دارویی به مرحله اجرا درآوریم. روش تحقیق: به منظور بررسی اثر چند گیاه دارویی بر سیستم ایمنی ماهی قزل آلای رنگین کمان، آزمایشی در قالب یک طرح کاملاً تصادفی با 7 تیمار و 3 تکرار و در هر تکرار 12 قطعه ماهی در مجموع با 252 قطعه ماهی با میانگین وزن 5 ±50 گرم به مدت 8 هفته انجام گرفت. هفت تیمار آزمایش شامل 1% اسانس مرزه بختیاری، آویشن دنایی، مرزه خوزستانی، زرین گیاه، پونه کوهی، جیره معمولی+روغن زیتون و جیره معمولی (شاهد) بود. نتایج و بحث: نتایج این پژوهش نشان داد که بالاترین میزان صفات مربوط به شاخص های ایمنی یعنی درصد فاگوسیتوز و تعداد جرم فاگوسیته شده و میزان Igm در اثر تغذیه اسانس 3 گیاه پونه کوهی، مرزه خوزستانی، زرین گیاه بود (05/0>p). میزان لنفوسیت ها و هتروفیل های خون ماهی های تغذیه شده با جیره حاوی اسانس مرزه بختیاری در مقایسه با شاهد به ترتیب کاهش و افزایش مشاهده گردید (05/0>p). توصیه کاربردی/ صنعتی: در مجموع نتایج این تحقیق نشان داد که افزودن 1% اسانس گیاهان دارویی به خصوص پونه و مزره بختیاری به روغن زیتون به همراه جیره غذایی ماهی قزل آلای رنگین کمان سبب تقویت سیستم ایمنی ماهی می شود. Manuscript profile
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        11 - تغییرات در ترکیبات اسانس پونه کوهی Menthalongifolia(L.) Huds رشد یافته در ایران
        احمدرضا گلپرور امین هادی پناه محمدمهدی قیصری سعید صالحی رضا خلیلی اذر امید قاسمی
        مقدمه و هدف :پونه کوهی با نام علمی (MenthaLongifoliaL.) متعلق به خانواده نعناع (Lamiaceae)می­باشد.هدف از این مطالعه شناسایی عملکرد اسانس و ترکیبات شیمیایی موجود در قسمتهای هوایی گیاه بخصوص برگها، ساقه و گلهای گیاهپونه کوهیدر شرایط آب و هوایی ایران می­باشد. تحقیق More
        مقدمه و هدف :پونه کوهی با نام علمی (MenthaLongifoliaL.) متعلق به خانواده نعناع (Lamiaceae)می­باشد.هدف از این مطالعه شناسایی عملکرد اسانس و ترکیبات شیمیایی موجود در قسمتهای هوایی گیاه بخصوص برگها، ساقه و گلهای گیاهپونه کوهیدر شرایط آب و هوایی ایران می­باشد. تحقیق:استخراج اسانس به روش تقطیر با آب و به کمک دستگاه اسانس گیری طرح کلونجر صورت گرفت و میزان اسانس به وسیله دستگاه کروماتوگرافی گازی متصل به طیف سنج جرمی (GC/MS) مورد تجزیه و شناسایی قرار گرفت. تفاوت معنی­داری در عملکرد اسانس از قسمتهای هوایی (برگ، ساقه و گل)  مورد بررسی قرار گرفت. نتایج و بحث:نتایج بدست آمده از این تحقیق نشان داد که 34/1، 76/0 و 97/0 درصد در اسانس برگ، ساقه و گلهای پونه کوهی به ترتیب شناسایی شد. تفاوت معنی داری در سطح یک درصد در بین ترکیبات شیمیایی بدست آمد. ترکیبات اصلیاسانس برگهای گیاه شامل1,8-cineole(16/37 %)،piperitenone oxide(97/18 %)،sabinene(94/13 %)، α-pinene(92/8 %) و pulegone(14/6 %) بدست آمد.ترکیبات اصلیاسانس ساقه گیاه شامل1,8-cineole(81/36 %)،pulegone(61/18 %)، piperitenone oxide(21/12 %) و sabinene(05/7 %) حاصل شد. ترکیبات اصلیاسانس گلهای گیاه شاملpiperitenone oxide(67/37 %)،1,8-cineole(02/23 %)،sabinene(56/13 %) و α-pinene(45/10 %)بدست آمد. توصیه کاربردی/صنعتی:خصوصیات مورفولوژیکی می­تواند تحت شرایط فاکتورهای مختلف یعنی محیطی و ژنتیکی قرار گیرد.   Manuscript profile