List of Articles Endometrial stem cells

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  1 - Studying the therapeutic effect of artemisinin along with endometrial stem cells containing TSP-1 gene on Alzheimer's disease in streptozotocin-induced Alzheimer's-diabetes model of rat
  پروین Poorgholam پریچهره Yaghmaei مهدی Noureddini زهرا Hajebrahimi
  10.30495/jcp.2023.22496
  Alzheimer's is the most common neurodegenerative disease of the elderly. Oxidative stress and inflammation play an important role in Alzheimer's disease. The risk of Alzheimer's disease is high in diabetic people, and insulin resistance occurs in the brain of Alzheimer' More

  Alzheimer's is the most common neurodegenerative disease of the elderly. Oxidative stress and inflammation play an important role in Alzheimer's disease. The risk of Alzheimer's disease is high in diabetic people, and insulin resistance occurs in the brain of Alzheimer's patients. Accumulation of amyloid plaques leads to angiogenesis and increased permeability of the vessels. Today, medicinal plants are receiving attention due to fewer side effects than chemical drugs. In the present study, the protective effect of Artemisia herbal product (artemisinin) along with endometrial stem cells containing the antiangiogenesis gene (thrombospondins-1 or TSP-1) was investigated in Alzheimer's disease induced by streptozotocin in a diabetic male rat laboratory model. 3 days after the induction of Alzheimer's and diabetes using streptozotocin, the animals received stem cells intranasally and then received artemisinin for one month (50 mg/kg). Initial and final weight, blood sugar, and interleukin-6 serum levels were measured, and brain amyloid plaques were examined by hematoxylin-eosin staining. The results showed that the induction of Alzheimer's and diabetes led to a significant decrease in body weight and an increase in blood sugar, interleukin-6, and brain amyloid plaques. Treatment with artemisinin and stem cells, separately and simultaneously, improved these parameters. The findings of this research showed that human endometrial stem cells that contain the TSP-1 gene can be used as a potential source of stem cells along with natural antioxidants artemisinin to reduce the symptoms of Alzheimer's disease. Manuscript profile
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  2 - Evaluation of adhession and viability of endometrial stem cells-derived osteoblast-like cells cultured on PLGA/HA scaffold
  mojdeh salehi jafar ai nasrin lotfibakhshaiesh shiva irani سمیه ابراهیمی باروق
  Introduction: The engineered tissue structure includes three components: cells, signaling pathway and scaffold. The polylactide-co-glycolide/hydroxyapatite (PLGA/HA) has attracted much attention due to their optimal properties because of using PLGA polymer and hydroxyap More

  Introduction: The engineered tissue structure includes three components: cells, signaling pathway and scaffold. The polylactide-co-glycolide/hydroxyapatite (PLGA/HA) has attracted much attention due to their optimal properties because of using PLGA polymer and hydroxyapatite. The aim of this study was to evaluate the biocompatibility effect of PLGA/HA scafffold on osteoblast differentiated from hEnSCs. We aimed to evallute the adhesin of cells on scaffolds. hEnSCs were isolated from human endometrium tissue and induced the osteogenic differentiation, then the differentiated cells were cultured on PLGA/HA synthetic scaffolds. A nanocomposite scaffolds based on PLGA/HA were fabricated by electrospinning methode and behavior of differentiated osteoblast cells was evaluated after seeding cells on this scaffold. Osteogenesis was investigated in terms of attachment, alkaline phosphatase activity and gene expression. Morphogenic of cells in scaffold was evaluated by SEM and biocampatibility of this scaffold was confirmed by MTT assay. The alkaline phosphatase activity confirmed osteoblast differentiation. SEM studies showed that the surface properties of scaffold were desirable and the cells had the ability to attach and proliferate better on the nanocomposite scaffolds. It has been shown that the nanocomposite scaffolds have appropriate properties to support the attachment of differentiated osteoblast cells. Manuscript profile
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  3 - Experimental Study on Evaluation of Effects of Implantation Site of Mesenchymal Stem Cells/scaffold on Recovery of Diabetic in a Rat Model
  elham hoveizi
  10.30495/jdb.2023.1966697.1331
  Diabetes Mellitus is an autoimmune and chronic disorder that has spread rapidly all over the world due to lifestyle and obesity. In this study, we are trying to find an effective step in controlling diabetes by designing an engineered tissue and grafting it to different More

  Diabetes Mellitus is an autoimmune and chronic disorder that has spread rapidly all over the world due to lifestyle and obesity. In this study, we are trying to find an effective step in controlling diabetes by designing an engineered tissue and grafting it to different sites in the animal model. Uterine endometrial mesenchymal stem cells (EnMSCs) were prepared using the enzymatic extraction method and PAN nanofiber scaffold by electrospinning method. EnMSCs were cultured on the scaffold and transplanted into diabetic rats treated with streptozotocin. The engineered tissue was transplanted in one group peritoneally in the abdominal cavity and the other group subcutaneously. Also, in another group of rats, EnMSCs were injected through the tail. After transplantation, blood glucose, insulin, and weight of rats were measured. The findings of the present study showed that the method and area of stem cell transplantation play an important role in the control of diabetes. In the groups receiving EnMSCs, glucose concentration, blood insulin level, and body weight were improved compared to the control group. Compared to other groups, glucose concentration decreased significantly and blood insulin level and body weight increased significantly in rats receiving a peritoneal transplant. In the subcutaneous transplant group and the injection group, there was no significant difference in the investigated criteria. According to the results of this study, transplantation of EnMSCs using PAN scaffold in the peritoneal site can be suggested for the treatment of diabetes, although more studies are needed in this field to provide a complete treatment. Manuscript profile
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  4 - Investigating the combined effect of endostatin along with ‎endometrial stem cells expressing TSP-1 anti-angiogenic gene on ‎angiogenic induce factors on the treatment of prostate cancer in rats
  hossein Attar shakeri Esmail Fattahi Mohammadreza Nowroozi Seyed Ali Momeni Abbas Piryai
  Prostate cancer is the second leading cause of cancer deaths in men. Androgen ‎deprivation therapy has been identified to induce oxidative stress in prostate cancer, ‎leading to reactivation of androgen receptor (AR) signaling. Thus, antioxidant therapies ‎h More

  Prostate cancer is the second leading cause of cancer deaths in men. Androgen ‎deprivation therapy has been identified to induce oxidative stress in prostate cancer, ‎leading to reactivation of androgen receptor (AR) signaling. Thus, antioxidant therapies ‎have gained attention as adjuvants for this cancer. Here, we report for the first time that ‎endostatin along with endometrial stem cells expressing TSP-1 anti-angiogenic gene ‎improved androgen- phenotype and genotype in rats prostate cancer cells.‎‏ ‏Forty male ‎rats (n=8) weighing approximately 95-100 g were purchased from Pasteur Inistitute of ‎Tehran . Animals were suffered to prostate cancer by injecting LNCaP cell line for 12 ‎weeks. Prostate cancer animals were immediately received endostatin for 28 days. ‎Prostate cancer animals were received stem cell containing the Tsp-1 anti-angiogenesis ‎gene. Histological examinations were measured. The levels of IL8 and VEGF serum ‎were assayed.‎‏ ‏In group receiving cells and drug, amount and height of acinus wall folds ‎decreased compared to the patient group and the shape of the vesicles was clear and the ‎epithelial lining were cohesive and regular. The levels of IL8 and VEGF serum were ‎significantly decreased in combination group than to prostate cancer group (P≤0.01). ‎ Manuscript profile
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  5 - The Effect of Endometrial Stem Cells with Pomalidomide on Serum Glutathione and Lipids in 6-OHDA Induced Rat Model of Parkinson
  Elham Ghasemi deligani Maryam Khosravi Mahmoud Salami Zavareh Ramin Haji Khani
  10.22034/ascij.2022.1939521.1303
  Parkinson’s disease is the second most common age-related neurodegenerative disease with no identified effective treatment. The aim of this study was to evaluate the effects of human endometrial stem cells with the anti-inflammatory drug pomalidomide on glutathion More

  Parkinson’s disease is the second most common age-related neurodegenerative disease with no identified effective treatment. The aim of this study was to evaluate the effects of human endometrial stem cells with the anti-inflammatory drug pomalidomide on glutathione (GSH) and serum lipids (total cholesterol,TC and low density lipoprotein, LDL)) in the Parkinson’s model in male Wistar rats. Male rats were randomly assigned to five groups (8 animals in each group) including: control, Parkinson’s, and 3 experimental Parkinson’s groups receiving stem cells, pomalidomide, and a combination of stem cells and pomalidomide. Parkinson’s was induced by injection of 6-hydroxy dopamine (concentration 6 μg) in the striatum by stereotaxic method. In the fourth week after surgery, the three experimental groups were treated with  4 mg/kg/daily of pomalidomide, 100,000  stem cells through intranasal route, and a combination of pomalidomide at a dose of 4 mg/kg/daily and 100,000 stem cells. At the end of day 28, blood samples were taken from the groups and factors were measured. The results revealed a significant increase in glutathione among the treated groups compared to the Parkinson’s group. There was also a significant decrease in glutathione in Parkinson’s group compared to the control group. Comparison between Parkinson’s group and the group treated with pomalidomide and endometrial stem cells showed a significant reduction in cholesterol. Moreover, LDL levels showed a significant decrease in LDL levels in the treated groups compared to the Parkinson’s group. Considering the improvement of the measured factors in Parkinson’s rat, allogeneic stem cells can be used as a potential source along with pomalidomide in future research for the treatment of Parkinson’s disease. Manuscript profile