Investigating the combined effect of endostatin along with ‎endometrial stem cells expressing TSP-1 anti-angiogenic gene on ‎angiogenic induce factors on the treatment of prostate cancer in rats

Subject Areas : Journal of Animal Biology

hossein Attar shakeri ¹ , Esmail Fattahi ² , Mohammadreza Nowroozi ³ , Seyed Ali Momeni ⁴ , Abbas Piryai ⁵

1 - Department of Animal Sciences, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
2 - Department of Animal Sciences, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
3 - Department of Animal Sciences, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
4 - Uro-Oncology Research Center, Tehran University of Medical Sciences, Tehran, Iran
5 - Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Received: 2020-11-11 Accepted : 2020-11-25 Published : 2021-02-19

Keywords: Rat, prostate cancer, endometrial stem cells, Endostatin, Anti-angiogenesis,

Abstract :

Prostate cancer is the second leading cause of cancer deaths in men. Androgen ‎deprivation therapy has been identified to induce oxidative stress in prostate cancer, ‎leading to reactivation of androgen receptor (AR) signaling. Thus, antioxidant therapies ‎have gained attention as adjuvants for this cancer. Here, we report for the first time that ‎endostatin along with endometrial stem cells expressing TSP-1 anti-angiogenic gene ‎improved androgen- phenotype and genotype in rats prostate cancer cells.‎‏ ‏Forty male ‎rats (n=8) weighing approximately 95-100 g were purchased from Pasteur Inistitute of ‎Tehran . Animals were suffered to prostate cancer by injecting LNCaP cell line for 12 ‎weeks. Prostate cancer animals were immediately received endostatin for 28 days. ‎Prostate cancer animals were received stem cell containing the Tsp-1 anti-angiogenesis ‎gene. Histological examinations were measured. The levels of IL8 and VEGF serum ‎were assayed.‎‏ ‏In group receiving cells and drug, amount and height of acinus wall folds ‎decreased compared to the patient group and the shape of the vesicles was clear and the ‎epithelial lining were cohesive and regular. The levels of IL8 and VEGF serum were ‎significantly decreased in combination group than to prostate cancer group (P≤0.01). ‎