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        1 - The impact of salt stress on growth and some biochemical parameters of Echinaceae purpurea L.
        Mahnaz Aghdasi Mohammad Fatemi Asieh Asadi
        Echinaceae prupurea, belongs to the Asteraceae family and is a perennial herb with different medicinal properties. The aim of the present study was to investigate the effect of salt stress on growth and some important biochemical parameters of this plant. For this purpo More
        Echinaceae prupurea, belongs to the Asteraceae family and is a perennial herb with different medicinal properties. The aim of the present study was to investigate the effect of salt stress on growth and some important biochemical parameters of this plant. For this purpose, Echinaceae prupurea plantlets were grown in Hoagland medium supplemented with different concentrations of NaCl (including 10, 50, 100, 300, and 500 mM) for 20 days. The experiment was carried out in a completely randomized design with five replications. The obtained result showed that the effects of different concentrations of NaCl were not significant on shoot dry weights. But root fresh and dry weights exhibited a significant increasing trend by 25 mM NaCl treatment. Meanwhile, the current data showed that increasing salt concentration in the medium caused an increase and decrease in soluble sugar level in shoots and roots, respectively. While salt stress did not show any significant effect on catalase enzyme activity, peroxidase enzyme activity was significantly increased by 75 mM NaCl treatment, in both shoots and roots. On the other hands, salinity treatment significantly decreased and increased total phenol levels in shoots and roots, respectively. The data from HPLC analysis demonstrated that the highest level of cichoric acid (1.3 mg/g dry weight) was observed in roots of control samples. While NaCl at the level of 25 and 50 mM concentrations did not show any significant effect on cichoric acid amount, it was significantly decreased by 75 mMN aCl treatment. Moreover, 50 mM NaCl treatment enhanced 2 and 5 fold caffeic acid (precursor of cichoric acid) and chlorogenic acid level in root organ, respectively. It seems salt stress can increase the important medicinal secondary metabolites of Echinaceae purpurea. Manuscript profile
      • Open Access Article

        2 - The effect of harvesting time on some phytochemical characteristics of artichoke (Cynara scolymus L.) medicinal plant leaves
        Marziyeh Allahdadi Laleh Mosharraf
        Artichoke (Cynara scolymus L.) is a perennial plant that belongs to the Asteraceae family. It has a special place in the pharmaceutical and food industries all over the world. In order to evaluate some phytochemical characteristics of artichoke leaves at different growt More
        Artichoke (Cynara scolymus L.) is a perennial plant that belongs to the Asteraceae family. It has a special place in the pharmaceutical and food industries all over the world. In order to evaluate some phytochemical characteristics of artichoke leaves at different growth stages, an experiment was conducted by using a randomized complete blocks design (RCBD) with three replications in Isfahan Agricultural and Natural Resources center during two consecutive years (2014-2015 and 2015-2016). In the second year, the leaves were collected at three growth stages: vegetative, heading and flowering in the second year. After harvesting, some quality factors such as their total phenolic content (Folin–Ciocalteu method), total flavonoids (Colorimetric Method of Aluminum Chloride), chlorogenic acid content (HPLC method) and antioxidant activity (DPPH free radical scavenging activity and reducing power) were measured. The results showed that the phytochemical and antioxidant properties of artichoke leaves were different at the three growth stages. The highest amount of phenolic content (76.25 mg GAE g-1 DW), total flavonoid (1.28 mg QE g-1 DW), chlorogenic acid content (2.25 % DM), DPPH (92.34 %) and reducing power (2.16) of methanolic extract was observed in heading stage. There was a positive and significant correlation between total phenolic content and antioxidant activity. Also, the relationship between chlorogenic acid content and DPPH and reducing power was positive and significant. According to the results, the harvesting of artichoke leaves at the heading stage is better than other growth stages due to their favorable characteristics. Manuscript profile
      • Open Access Article

        3 - Evaluation and comparison of the most important secondary metabolites of methanolic extract of different artichoke (Cynara scolymus L.) organs under drought stress and planting density
        manouchehr tahmasebi Yousef Hamidoghli mohammadbagher rezaee alireza hossaini
        In this project, in order to evaluate and compare the most important phenolic compounds in extract of different artichoke (Cynara scolymus L.) organs under water stress and planting density, an experiment was conducted as split plot in a randomized complete block design More
        In this project, in order to evaluate and compare the most important phenolic compounds in extract of different artichoke (Cynara scolymus L.) organs under water stress and planting density, an experiment was conducted as split plot in a randomized complete block design with three replications at Sarabelle-Ilam Agricultural Research Station during 2014-2015. The treatments comprised four irrigation regime (25, 50, 75 and 100 % of the water requirement of the plant) in the main plots and four densities(10, 20, 30 and 40 thousand bushes in hectare with 0.5, 0.67, 1 and 2m interval within the row, respectively, and 0.5m between rows) in sub plots. herbal sampels were harvested in the second year, and after drying in room temperature were used for methalonic extract. Identification and amount of naringin, caffeic and chlorogenic acid was done with HPLC methode. According to the obtained results, increasing drought stress caused 78 and 11% reduction in naringin amount compared to control treatment of bud and leaf extract, respectively. The maximum amount of caffeic acid (0.74 mg/g dry matter) was observed in leaf extract and interaction effect of full irrigation and 10 thousand bushes in hectare. Drought improved caffeic and chlorogenic acid biosynthesis of bud, but in interaction effect with density, only chlorogenic acid had the highest amount in sever water stress and 10 thousand bushes in hectare .Among consumed organs extract, caffeic acid and naringin had the highest amount in leaf extract, and chlorogenic acid in bud extract Manuscript profile
      • Open Access Article

        4 - مروری بر جنبه های مختلف گیاه دارویی کنگرفرنگی (Cynara scolymus L.)
        مرضیه الله دادی
        مقدمه و هدف: کنگرفرنگی(Cynara scolymus L.) گیاهیدارویی ازتیره Asteraceae است. این گیاه بومی جنوب مدیترانه و شمال آفریقا می‌باشد و امروزه در بسیاری از مناطق دنیا جهت مصارف دارویی و غذایی کشت می‌شود. کاپیتول‌ها بخش خوراکی گیاه هستند و برگ‏های آن در صنایع داروسازی More
        مقدمه و هدف: کنگرفرنگی(Cynara scolymus L.) گیاهیدارویی ازتیره Asteraceae است. این گیاه بومی جنوب مدیترانه و شمال آفریقا می‌باشد و امروزه در بسیاری از مناطق دنیا جهت مصارف دارویی و غذایی کشت می‌شود. کاپیتول‌ها بخش خوراکی گیاه هستند و برگ‏های آن در صنایع داروسازی مورد استفاده قرار می‏گیرند. کنگر فرنگی به‏دلیل داشتن ترکیبات پلی‏فنلی و فلاونوئیدی مختلف با اهمیت دارویی وآنتی‏اکسیدانی، درصنایع دارویی و طب سنتی جایگاه خاصی دارد. برگ کنگرفرنگی دارای خواص آنتی­اکسیداتیو، آنتی­باکتریال، تخلیه کننده صفرا و نیز خاصیت مدری می­باشد. بعلاوه، عصاره کنگرفرنگی بدلیل مهار بیوسنتز کلسترول منجر به کاهش کلسترول و چربی خون می­شود. اسیدکلروژنیک و سینارین ترکیبات عمده گیاه محسوب می­شوند که خواص دارویی این گیاه عمدتا به آنها مربوط می­باشد. مطالعه حاضر به بررسی جنبه­های مختلف این گیاه پرداخته است. توصیه کاربردی/صنعتی: گیاه کنگر فرنگی دارای فیتوکمیکال­های مختلف در مقادیر بالا است و می­توان از آن داروهایی با منشاء گیاهی تولید کرد. همچنین انجام مطالعات بیشتر در مورد خواص مفید و ناشناخته دیگر این گیاه چند منظوره ضروری به نظر می‌رسد.  Manuscript profile
      • Open Access Article

        5 - Evaluation of the phenolic compounds in the aerial part and roots of chicory (Cichorium intybus L.) collected from different localities of the Iran using high performance liquid chromatography (HPLC)
        mahshid Rahimifard fatemeh sefidkon somayeh fekri qomi مریم مکی زاده تفتی
        In this article, a phytochemical investigation including identification and measurement of some phenolic compounds in the hydroalcoholic extracts of the Cichorium intybus L. collected from 6 localities was carried out. Among the studied compounds, chicoric acid (0.55 to More
        In this article, a phytochemical investigation including identification and measurement of some phenolic compounds in the hydroalcoholic extracts of the Cichorium intybus L. collected from 6 localities was carried out. Among the studied compounds, chicoric acid (0.55 to 6.04 mg per gram of dry plant), chlorogenic acid (0.50 to 5.37 mg per gram of dry plant), and rutin (0.56 to 2.25 mg per gram of dry plant) were found in extracts of aerial parts and chicoric acid (0.03% to 0.29 mg per gram of dry plant) and chlorogenic acid (0.84% to 2.88 mg per gram of dry plant) were found in the roots of all samples. Among the studied populations, the highest amount of major compounds (chicoric acid and chlorogenic acid) were observed in the aerial parts of the sample collected from the National Botanical Garden of Iran with 6.04 mg of chicoric acid and 5.37 mg chlorogenic acid per gram of dry plant. Manuscript profile