List of Articles BCL-2

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  1 - Study of rosemary extract on BCL2 and BAX gene expression in canine mammary gland carcinoma cell line (CF41.Mg)
  Shabani, S., Mortazavi, P. .
  Apoptosis is named genetically programmed cell death and have special role in physiological and pathological conditions. BAX protein as a key protein in the apoptosis induced by various factors in the apoptosis pathway acts and BCL-2 have anti-apoptotic effect in respon More

  Apoptosis is named genetically programmed cell death and have special role in physiological and pathological conditions. BAX protein as a key protein in the apoptosis induced by various factors in the apoptosis pathway acts and BCL-2 have anti-apoptotic effect in response to various stimuli of the mitochondrial apoptosis by preventing the release of cytochrome C applies. In this study, the effect of rosemary extracts on the expression of BCL-2 and BAX genes on breast cancer cells dogs (CF41.Mg). in in vitro examined and the control group (which cell line CF41.Mg without drugs) were compared Cells at doses of 5,10,25,50 and 100 micromole rosemary extract for 24,48 and 72 hours were exposed then by using MTT, the effect of drug on cell survival were analyzed. The tests in which different levels of rosemary extract was added to the cell culture, it was determined that rosemary extract induced cell death after 48 hours’ maximum amount to be allocated. The result suggests that the antitumor activity of the extract after 48 hours at a dose 25 mg/ml is more effective. The results of this study confirm the inhibitory effect of rosemary extract on canine mammary tumor cells (CF41.Mg). Manuscript profile
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  2 - The effect of endurance training and consumption of nettle extract on the gene expression of BCL-2 and TNF-α in mice with melanoma
  lila mosavi barari barari asieh Abbasi Daloii hossien Abed Natanzi
  Inroduction & Objective: : Melanoma is the most serious type of skin cancer, which is made up of melanin pigment cells.The aim of this study was to evaluate the effect of endurance training and nettle consumption on BCL-2 and TNF-α gene expression in mice with More

  Inroduction & Objective: : Melanoma is the most serious type of skin cancer, which is made up of melanin pigment cells.The aim of this study was to evaluate the effect of endurance training and nettle consumption on BCL-2 and TNF-α gene expression in mice with melanoma.Material and Methods: The subjects included male Wistar rats that after two weeks of adjustment were randomly divided into 4 groups including: control, exercise, extract and exercise + extract. Exercise program included 30 minute of running on a treadmill was steep and at a speed of 16 meters per minute for the first week, and every week one meter per minute was added until the eighth week it reached 22 meters per minute. One week after melanoma induction, the experimental group consumed 30 mg / kg / day of nettle ethanol extract orally for 8 weeks. RT PCR was used to measure gene expression.Results: Data analysis showed that consumption of nettle extract and endurance training significantly reduced the expression level of BCL-2 and TNF-α genes in mice with melanoma in different groups (p = 0.000, p = 0.025, respectively). The results also showed that the difference in the level of changes in BCL-2 and TNF-α gene expression between the exercise and extract group with the control group (p = 0.002, p = 0.014, respectively) was significantly reduced.Conclusion: Regular physical activity combined with consumption of nettle extract can probably reduce anti-apoptotic factors and cause apoptosis in cancer cells by reducing inflammatory factors. Manuscript profile
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  3 - Effect of Green Tea (Camellia sinensis) Extract on Bcl_2 and Bax Genes Expression in Gastric Cancerous Cell Line (AGS)
  maryam Haghgoo Khomami Zahra Deilami
  Inroduction & Objective: Gastric cancer is second leading cause of cancer related death in the world. Use of herbal drugs because of having fewer side effects is of importance. The anticancer effects of catechin polyphenols in green tea have key role in promoting ca More

  Inroduction & Objective: Gastric cancer is second leading cause of cancer related death in the world. Use of herbal drugs because of having fewer side effects is of importance. The anticancer effects of catechin polyphenols in green tea have key role in promoting cancer cells to apoptosis. The aim of this study is to determine the changes in the expression rate of Bax and Bcl_2 genes in AGS cells after treatment with green tea extract. Materials and Methods: Concentrations of 800, 1200, 2000  μg /ml of Green Tea extraction were treated in AGS cells as two groups 48 and 72 hours. Extraction of  RNA, synthesis of cDNA and the study of genes expression was performed by Real time PCR and also GAPDH gene was used as reference gene. Results: The results of Real time PCR data analysis by 2- Ct method shows significant increase expression rate of  Bax / Bcl_2 genes in doses of 800, 1200 and 2000 μg / ml for times of 48 and 72 hours. Conclusion: Green tea is the reason of increasing Bax/Bcl_2 ratio which it is dependent to concentration and time, and it can lead cancerous cell to programed cell death. This extract can be useful in the treatment of gastric cancer for patients with this cancer. To clarify the effective molecules and their mechanisms, further studies are undertaken on animal models and humans. Manuscript profile
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  4 - The interactive effect of swimming training and vitamin D supplements on Bcl-2 gene expression in heart tissue of cadmium-poisoned rats
  Amir Atefat Izadi Javid Ouji
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  5 - Investigation of hydroalcoholic extract effect of Cordia Myxa fruit on the expression of BCL-2 and BAX genes in the apoptotic pathway and cell proliferation in human lung cancer cell line.
  NAHID ASKARI Kian Aghaabbasi elham rezvannejad
  10.30495/jdb.2023.1987391.1368
  Cancer is one of the most important causes of death worldwide. On the other hand, the therapeutic use of medicinal plants has increased during recent years. For this purpose, the aim of the present research was to investigate the effect of a Cordia myxa fruit extract on More

  Cancer is one of the most important causes of death worldwide. On the other hand, the therapeutic use of medicinal plants has increased during recent years. For this purpose, the aim of the present research was to investigate the effect of a Cordia myxa fruit extract on cellular proliferation and apoptosis gene expression (BCL-2 and BAX) in lung cancer cell line (A549). The plant was collected and Hydroalcoholic extract was prepared by macerating method. Various concentrations of Cordia myxa extracts (of 0.25, 0.5, 1, 2, 4 mg/mL) obtained to treat lung cancer and fibroblast cell lines. Cells were examined by MTT-assay and Real Time RT-qPCR to determine the plant extract-induced cytotoxicity in cell lines with and without treatment The results showed that, the concentration of ≥2 mg/ml extract had a significant lethal effect on cancer cells. The expression of BAX gene in the treated cell line increased and the expression of BCL-2 gene decreased compared to the control. The extract of Cordia myxa fruit didn’t show toxicity effects when used in low concentrations. However, this extract demonstrated the lower cytotoxicity against normal fibroblast cells. The combination therapy can be less toxic if both (plant extract and chemotherapeutic agent) use to treat cancers. It can be useful with fewer side effects of chemotherapeutic agents. Manuscript profile
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  6 - Effect of 12-week Aerobic Exercise on the Tumor Size and Expression of HIF-1α, BCL-2, Mir-15a, and VEGF Genes in BALB/C Female Mice with Breast Cancer
  Shaima Banoon Saade Abdalkareem Jasim Abdolmajid Ghasemian
  10.22034/jchr.2023.1981082.1692
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  7 - The Effect of 12 Weeks of Green Tea Extract Consumption on Expression of Bax and bcl-2 Genes of Old ’Rats’ Heart Tissue in Response to Acute Exhaustive Exercise
  Ahmad Kazemi Naser Behpoor Ahmad Hematfar
  10.22034/ascij.2022.1943959.1332
  The incidence of heart disease increases with age. Green tea is an antioxidant that can prevent cellular senescence. Mitochondria play a vital role in the intracellular pathway of apoptosis. Bax and bcl-2 proteins are the main regulators of mitochondrial apoptosis. Thus More

  The incidence of heart disease increases with age. Green tea is an antioxidant that can prevent cellular senescence. Mitochondria play a vital role in the intracellular pathway of apoptosis. Bax and bcl-2 proteins are the main regulators of mitochondrial apoptosis. Thus, the present study is aimed at investigating the effect of 12 weeks of green tea extract consumption on expression Bax and bcl-2 genes of old rats’ heart tissue in response to acute exhaustive exercise. Thirty-two male rats were randomly assigned to two groups of supplement and non-supplement, each consisting of 16 rats. The supplement group received green tea extract for 12 weeks. At the end of the 12 weeks, each of these groups was further divided into two groups of rest and acute exhaustive exercise. The non-supplement with the rest group was called the control group. The non-supplement with exercise group was called exercise group, the supplement group with rest was called the supplement group and finally, the last group was entitled to supplement with exercise. After 12 weeks of taking green tea extract, one session of the acute exhaustive exercise was held on the treadmill. The real-time PCR method was used to measure the expression of Bax and bcl-2 genes. The results showed that 12 weeks of green tea extract consumption significantly reduced Bax gene expression and Bax to bcl-2 gene expression ratio and significantly increased cardiac tissue bcl-2 gene expression in response to acute exhaustive exercise. About acute exhaustive exercise session, it significantly increased Bax gene expression and Bax to bcl-2 gene expression ratio as well as significantly decreasing bcl-2 gene expression in heart tissue. The study concludes that 12 weeks of green tea extract can reduce Bax gene expression and Bax to bcl-2 gene expression ratio and significantly increase cardiac tissue bcl-2 gene expression at rest and after acute exhaustive exercise   Manuscript profile