Effect of Green Tea (Camellia sinensis) Extract on Bcl_2 and Bax Genes Expression in Gastric Cancerous Cell Line (AGS)

Subject Areas :

maryam  Haghgoo Khomami ¹ (IMsc, Faculty of Basic Science, Islamic Azad University, Zanjan Branch, Zanjan,Iran)
Zahra  Deilami ² (Assistant professor, Faculty of Basic Science, Islamic Azad University, Zanjan Branch, Zanjan,Iran)

Received: 2021-07-23 Accepted : 2021-10-07 Published : 2021-09-23

Keywords:

Abstract :

Inroduction & Objective: Gastric cancer is second leading cause of cancer related death in the world. Use of herbal drugs because of having fewer side effects is of importance. The anticancer effects of catechin polyphenols in green tea have key role in promoting cancer cells to apoptosis. The aim of this study is to determine the changes in the expression rate of Bax and Bcl_2 genes in AGS cells after treatment with green tea extract. Materials and Methods: Concentrations of 800, 1200, 2000  μg /ml of Green Tea extraction were treated in AGS cells as two groups 48 and 72 hours. Extraction of  RNA, synthesis of cDNA and the study of genes expression was performed by Real time PCR and also GAPDH gene was used as reference gene. Results: The results of Real time PCR data analysis by 2- Ct method shows significant increase expression rate of  Bax / Bcl_2 genes in doses of 800, 1200 and 2000 μg / ml for times of 48 and 72 hours. Conclusion: Green tea is the reason of increasing Bax/Bcl_2 ratio which it is dependent to concentration and time, and it can lead cancerous cell to programed cell death. This extract can be useful in the treatment of gastric cancer for patients with this cancer. To clarify the effective molecules and their mechanisms, further studies are undertaken on animal models and humans.

References:

1.Aceto, N,, Duss , S., MacDonald, G., Meyer, DS., Roloff, T-C., Hynes, N E. (2012). Co-expression of HER2 and HER3 receptor tyrosine kinases enhances invasion of breast cells via stimulation of interleukin-8 autocrine secretion. Breast Cancer Research, 14(5); R131.

2.Antonsson, B., Martinou, J-C. (2000). The Bcl-2 Protein Family. Experim Cell Research, 256(1);50-7.

3.Amini Sarteshnizi, N., Teimori, H., Zahri, S., Mobini Dehkordi, M., Khosravi, S., Amini Sarteshnizi, R. (2015). Effect of Chrysin on AGS human gastric cancer cell line. J Gorgan Univ Med Sci, 16(4);  63-8.

4.Evacuasiany, E., Ratnawati, H., Liana, L K., Widowati, W., Maesaroh, M., Mozef ,T. (2014). Cytotoxic and antioxidant activities of catechins inhibiting the malignancy of breast cancer, Oxid Antioxid Med Sci, 3(2); 141-6.  

5.Hoshyar, R., Bathaie, SZ., Sadeghizadeh, M. (2013). Crocin triggers the apoptosis through increasing the Bax/Bcl-2 ratio and caspase activation in human gastric adenocarcinoma, AGS, cells. DNA Cell Biol, 32(2); 50-7.

6.Hosainzadegan, H. , Ezzetpor, B. , Abdollahpor, F., Motamedy, M., Rashidipor, M . (2010). Study of cytotoxic activity of olive and green tea extracts on breast tumor cell line. J Ardabil Univ Med Sci, 10(4); 287-94. (persian)

7.Iravani, SH.(2013). Gastric cancer as a multifactorial disease. J Army Univ Med Sci., 11(2); 157-64. (persian)

8.Khosravi, A., Farid Hoseini, R., Jabbari Azad, F., Yousofzadeh, H., Moghiman, T., Vaez Tabasi, M. (2013). Inhibitory effects of saffron extract in preventing growth of colon adenocarcinoma cancerous cells. Medic J Mashhad Uni., 56(5);283-8. (persian).

9.Konturek, PC., Konturek, SJ., Sulekova, Z., Meixner, H., Bielanski, T., Starzynska, T.(2001).  Expression of hepatocyte growth factor, transforming growth factor alpha, apoptosis related proteins Bax and Bcl-2, and gastrin in human gastric cancer. Aliment Pharmacol Ther., 15(7); 989-99.

10.Koshida, Y., Saegusa, M., Okayasu, I. (1997). Apoptosis, cell proliferation and expression of Bcl-2 and Bax in gastric carcinomas: immuno histo chemical and clinico pathological study. British Journal of Cancer,75(3); 367.

11.Ma, J., Shi, M., Li, G., Wang, N., Wei, J., Wang, T. (2006). Regulation of Id1 expression by epigallocatechin‑3‑gallate and its effect on the proliferation and apoptosis of poorly differentiated AGS gastric cancer cells. International Journal of Oncology, 43;1052-8. 

12.Nishikawa, T., Nakajima, T., Moriguchi, M., Jo, M., Sekoguchi, S., Ishii, M.  (2006). A green tea polyphenol, epigalocatechin-3-gallate, induces apoptosis of human hepatocellular carcinoma, possibly through inhibition of Bcl-2 family proteins. Journal of Hepatology, 44(6); 1074-82.

13.Paul-Samojedny, M., Kokocin´ska, D., Samojedny, A., Mazurek, U., Partyka, R., Lorenz, Z. (2005). Expression of cell survival/death genes: Bcl-2 and Bax at the rate of colon cancer prognosis. BBA, 1741(1-2); 25-9.

14.Phukan, RK., Narain, K., Zomawia, E., Hazarika, NC., Mahanta, J.(2006). Dietary habits and stomach cancer in Mizoram, India. J Gastroenterol, 41(5); 418-24.

15.Qin,. J, Xie, L-P., Zheng, X-Y., Wang, Y-B., Bai, Y., Shen, H-F. (2007). A component of green tea, epigallocatechin-3-gallate, promotes apoptosis in T24 human bladder cancer cells via modulation of the PI3K/Akt pathway and Bcl-2 family proteins. BBRC, 354(4); 852-7.

16.Reed, J.C.(2000). Mechanisms of apoptosis. AJP, 157(5); 1415-30.

17.Singh, BN., Shankar, SH., Srivastava, RK. (2011). Green tea catechin, epigallocatechin-3-gallate (EGCG): Mechanisms, perspectives and clinical applications. Biochem Pharmacol, 82(12);1807-21.

18.Wang, YC., Bachrach, U.(2002). The specific anti-cancer activity of green tea epigallocatechin-3-gallate (EGCG). Amino Acids, 22(2); 131-43.