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      • Open Access Article

        1 - Investigation of heavy metals and important micro nutrients in milk powder and common baby food in Tehran market
        Mahdieh Khazai Shiva Dehghan Abkenar Nazanin Khakipour
        Introduction: Powdered milk or complementary foods, despite the importance ofbreastfeeding, play an important role in providing baby food in the world. The aim of thisstudy was to measure the amount of heavy metals in baby food. Heavy metals cadmium, lead,and arsenic ca More
        Introduction: Powdered milk or complementary foods, despite the importance ofbreastfeeding, play an important role in providing baby food in the world. The aim of thisstudy was to measure the amount of heavy metals in baby food. Heavy metals cadmium, lead,and arsenic cause the highest effects in humans, especially in children, and Fe, Mg, Ca, andZn are important micronutrients that, if deficient in nutrients, can have adverse effects onchildren's health.Materials and Methods: This study was performed on 8 baby food samples from 6 commoncommercial products in the market. Sampling was carried out by census method andmeasurement by induced plasma emission ICP-OES methods.Results: The results were reported by descriptive statistics. The results showed that arsenicranged between 2.8 to 8 ppm in all the samples examined that indicated excessiveconcentration of this heavy metal while the trace concentration of cadmium was observed.Lead concentration was in the range of 0.012 to 0.103 ppm. The concentration of iron,calcium, magnesium and zinc in some samples agreed with the standard values while in someindicated lower content.Conclusion: According to the results of this investigation, the amount of arsenic in baby foodsamples is higher than the specified limit. Some attention should be given to those samplesthat contain lower contents of calcium and zinc as noticed by standard values. Manuscript profile
      • Open Access Article

        2 - The Study on antibiotic resistance pattern of Cronobacter sakazakii strains isolated from infant formula and baby food types
        Samira abbasi bafetrat Mohammad Goli Hasan Momtaz
        Cronobacter sakazakii is one of the main pathogens that transmitted by feeding infants by contaminated infant formula and baby-food. This bacterium is one of the major causes of mortality, digestive diseases and meningitis in newborns. This study was performed in order More
        Cronobacter sakazakii is one of the main pathogens that transmitted by feeding infants by contaminated infant formula and baby-food. This bacterium is one of the major causes of mortality, digestive diseases and meningitis in newborns. This study was performed in order to detect microbiological and molecular contamination by Cronobacter sakazakii and study antibiotic resistance pattern of bacterial isolates. Two hundred samples of infant formula and baby food from deferent brands were collected and after microbial detection, bacteria’s isolates were detected, using PCR method. Then antibiotic resistance pattern of isolates were evaluated using disk diffusion method. Three samples from total 100 baby food (3%) and 5 samples from total 100 infant formula (5%) had Cronobacter sakazaki infection. A, B and C brands from infant formula and A, D brands from baby food were contaminated with Cronobacter sakazakii. B and C brands from infant formula (10% each of them) and A brand from baby food (8%) were infected with Cronobacter sakazakii. Isolates from Cronobacter sakazakii which isolate from infant formula and baby food demonstrated most Sensitivity to ciprofloxacin (20.38), meropenem (19.83) and imipenem (19.63) and most resistance to amoxicillin, ampicillin, tigecycline, ticarcillin, aztreonam and ceftazidime. Careful inspection on the raw material quality of infant formula and baby food as well as, exact supervision on production procedure, sanitation in addition to use natural antimicrobial can reduce Prevalence rate of this bacteria. Manuscript profile
      • Open Access Article

        3 - Detection of GMO soybean by Camv 35S Promoter and Lectin genes in baby food and soybean samples
        Mahsa Kavousi Fateme Salmani Zakaria
        In most countries today the area under cultivation of transgenic crops has increased due to the increasing population.In Iran, there is no system for labeling transgenic products.Although baby food is one of the most important needs for growth and health of the baby, it More
        In most countries today the area under cultivation of transgenic crops has increased due to the increasing population.In Iran, there is no system for labeling transgenic products.Although baby food is one of the most important needs for growth and health of the baby, it is no exception.Therefore, in this study,we try to identify the transgenic products by PCR by examining the internal control gene Lectin and Camv 35S Promoter gene transcriptome.25 soybean samples were collected from shops and Twenty-five samples of baby food were collected from pharmacies in Tehran. Samples were extracted with TLAB brand tissue extraction kit. After analyzing the concentration and quality of DNA extracted by the nanodrop machine, the samples were screened by PCR.25 soybean samples were analyzed for 23 of the total samples (%92) for the presence of Lectin internal gene and 15 samples from all samples (%63) had Camv 35S Promoter gene.Of the 25 samples of baby food, 20 (%83) were positive for the presence of the inner gene,Lectin positive, and 13 (%52) were positive for Camv 35S Promoter gene transformation index. None of the products containing Camv 35S Promoter gene, indicating that the product was transgenic, were not labeled.It can be concluded that the rate of transgenicity in the samples tested is significantly higher.In baby foods, the transgenicity rate of Camv 35S Promoter gene was higher in Iranian samples than in foreign samples. In soybean samples, Camv 35S Promoter gene was present in Iranian products, while none of the samples tested had transgenic label Manuscript profile
      • Open Access Article

        4 - Detection of transgenic soybeans through Nos terminator and Roundup ready genes in baby food samples
        Mahsa Kavousi Fatemeh Tayefekhani
        A large amount of soy consumed in Iran is imported and transgenic, but there is no system for labeling transgenic products, even baby food. In this study, PCR assay of NOS Terminator primer gene and Roundup Ready transgenic marker gene was used to identify Randup-resist More
        A large amount of soy consumed in Iran is imported and transgenic, but there is no system for labeling transgenic products, even baby food. In this study, PCR assay of NOS Terminator primer gene and Roundup Ready transgenic marker gene was used to identify Randup-resistant transgenic soybean in baby food. 25 samples of soy and 25 samples of baby food were collected randomly. After DNA extraction with TLAB brand tissue extraction kit and DNA concentration and quality, PCR was performed. The results showed that with 95% confidence we can say that there is no significant relationship between the presence of NOS Terminator in soy and baby food. While with 95% confidence we can say that there is a significant relationship between the presence of Roundup Ready in soy and baby food. The amount of transgenic soy used in baby food is less than transgenic soy studied in this study. It can be concluded that the rate of transgenics in the tested samples is significantly higher. None of these products had a transgenic label. Manuscript profile