Detection of GMO soybean by Camv 35S Promoter and Lectin genes in baby food and soybean samples

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

Mahsa Kavousi ¹ , Fateme Salmani Zakaria ²

1 - Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran
2 - Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran.

Received: 2021-12-17 Accepted : 2022-06-10 Published : 2022-08-23

Keywords: Labeling, Baby food, Transgenic, Lectin gene, Camv 35S Promoter gene,

Abstract :

In most countries today the area under cultivation of transgenic crops has increased due to the increasing population.In Iran, there is no system for labeling transgenic products.Although baby food is one of the most important needs for growth and health of the baby, it is no exception.Therefore, in this study,we try to identify the transgenic products by PCR by examining the internal control gene Lectin and Camv 35S Promoter gene transcriptome.25 soybean samples were collected from shops and Twenty-five samples of baby food were collected from pharmacies in Tehran. Samples were extracted with TLAB brand tissue extraction kit. After analyzing the concentration and quality of DNA extracted by the nanodrop machine, the samples were screened by PCR.25 soybean samples were analyzed for 23 of the total samples (%92) for the presence of Lectin internal gene and 15 samples from all samples (%63) had Camv 35S Promoter gene.Of the 25 samples of baby food, 20 (%83) were positive for the presence of the inner gene,Lectin positive, and 13 (%52) were positive for Camv 35S Promoter gene transformation index. None of the products containing Camv 35S Promoter gene, indicating that the product was transgenic, were not labeled.It can be concluded that the rate of transgenicity in the samples tested is significantly higher.In baby foods, the transgenicity rate of Camv 35S Promoter gene was higher in Iranian samples than in foreign samples. In soybean samples, Camv 35S Promoter gene was present in Iranian products, while none of the samples tested had transgenic label