Detection of transgenic soybeans through Nos terminator and Roundup ready genes in baby food samples
Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganismsMahsa Kavousi 1 , Fatemeh Tayefekhani 2
1 - Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran
2 - Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran.
Keywords: GMO, Soybean, Baby food, NOS Terminator, Roundup Ready,
Abstract :
A large amount of soy consumed in Iran is imported and transgenic, but there is no system for labeling transgenic products, even baby food. In this study, PCR assay of NOS Terminator primer gene and Roundup Ready transgenic marker gene was used to identify Randup-resistant transgenic soybean in baby food. 25 samples of soy and 25 samples of baby food were collected randomly. After DNA extraction with TLAB brand tissue extraction kit and DNA concentration and quality, PCR was performed. The results showed that with 95% confidence we can say that there is no significant relationship between the presence of NOS Terminator in soy and baby food. While with 95% confidence we can say that there is a significant relationship between the presence of Roundup Ready in soy and baby food. The amount of transgenic soy used in baby food is less than transgenic soy studied in this study. It can be concluded that the rate of transgenics in the tested samples is significantly higher. None of these products had a transgenic label.
