List of Articles دیسک دیفیوژن

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  1 - Antibiotic Resistance Pattern of Klebsiella Pneumoniae from Patients in Qaem Teaching Hospital, Mashhad University of Medical Sciences
  Ali Shamsazar Arshid Yousefi-Avarvand Farzad Khademi
  Klebsiella pneumoniae is a gram-negative, non-motile and capsular gram-negative bacillus. It is one of the most important human pathogens and is an important factor in causing nosocomial infections. The bacterium is inherently resistant to a number of antimicrobial agen More

  Klebsiella pneumoniae is a gram-negative, non-motile and capsular gram-negative bacillus. It is one of the most important human pathogens and is an important factor in causing nosocomial infections. The bacterium is inherently resistant to a number of antimicrobial agents. The aim of this study was to evaluate the antibiotic resistance pattern of Klebsiella pneumoniae isolated from patients referred to Ghaem hospital in Mashhad. A total of 274 Klebsiella pneumoniae isolates were collected from April to October 2013 from Qaem teaching hospital of Mashhad University of Medical Sciences. Identification of these isolates was performed using microbiological methods such as hot staining and differential media. Modified disk diffusion method (MDDM) was used to determine the susceptibility of bacteria to the antibiotics amoxicillin, nalidixic acid, cefotaxime, ceftizoxime, ceftriaxone, ceftazidime, ciprofloxazine, imipenem, and nitrofurantoin. In this study 175 isolates (87.63%) were from urine sample and 99 isolates (13.36%) were from different samples (blood, cerebrospinal fluid, ulcer, ascites, and secretions). Resistance of Klebsiella pneumoniae isolates to ciprofloxacin, imipenem, nitrofurantoin and gentamicin antibiotics were 71.46%, 9.56%, 3.39% and 30%, respectively. The results of this study indicate that resistance of Klebsiella pneumoniae isolates to different antibiotics is higher than previous studies. Further studies in this area could help guide us to better understand the antibiotic resistance of these infectious bacteria. Manuscript profile
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  2 - Studying the Antifungal effect of Hydroalcoholic extracts of three species of Medicinal plants from the growing areas of Meshgin Shahr against Clinical isolates of Candida Albicans.
  Hojjat Eghbal Mehdi Ahmadi Sabegh Neda Jahani Yousef Jahani Jelodar
  Method: dual mixture of Pune-Peppermint extract, Pune-Sourberry, Sour-Peppermint and Triple Blend of Hydroalcoholic extract of Pune, Peppermint and Sorghum used. The extracts were diluted with propylene glycol and in addition to pure extracts, concentrations of 10, 20, More

  Method: dual mixture of Pune-Peppermint extract, Pune-Sourberry, Sour-Peppermint and Triple Blend of Hydroalcoholic extract of Pune, Peppermint and Sorghum used. The extracts were diluted with propylene glycol and in addition to pure extracts, concentrations of 10, 20, 30, 40 and 50 mg / ml of extract were prepared. To study the effect of hydroalcoholic extracts of Pune, Mint and Sour and Dual Blend Triticale extracts of these three plants were used to grow Albicans candidate by antibiotic and MIC methodsResults: The results of this study showed that the triple mixture of Puneh, Mint and Savange extracts with a concentration of 50 mg / ml with a diameter of 32.2 mm had the highest diameter of the inhibition zone and the hydroalcoholic extract Peppermint at a concentration of 10 mg per milliliter with a diameter of 7 mm halo assigns the lowest diameter of the non-growth zone of Candida albicans. Also, the triple mixture of Puneh, Mint and Souri showed relatively similar results to fluconazole antibiotics.Conclusion: The results of the minimum inhibitory concentration showed that the lowest inhibitory concentration was related to the antibiotic fluconazole and the triple mixture of hydroalcoholic extract of Punea, Mint and Sardinia, and the lowest was the hydroalcoholic extract of Mint herb. Manuscript profile
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  3 - Antimicrobial effect of walnut leaves aqueous extract and comparison of disc diffusion and wells methods
  Afshin alimohammadi Afshin Javadi Elham Yaghma
  Today food safety is a public health issue. One of the methods of healthy food production is the use of natural materials. The use of essential oils and plant extracts as antibacterial and antifungal additives is one of these methods. Antimicrobial compositions obtained More

  Today food safety is a public health issue. One of the methods of healthy food production is the use of natural materials. The use of essential oils and plant extracts as antibacterial and antifungal additives is one of these methods. Antimicrobial compositions obtained from plants were removed by different mechanisms of antibiotics, in which the effective influences of walnut and its components are significant. The aim of this study was to evaluate the antimicrobial effect of walnut leaf extract on Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli), determine the optimal amount of extract and sensitivity to each of the bacteria against the extract and determine the most sensitive method of the study. In this study, the leaves was extracted from the walnut and then the antimicrobial properties of the extract and its optimal concentrations were measured using disc and well methods on S. aureus and E. coli. Based on the results, the extract of walnut leaves in the concentrations of 12.5% up to the above has antimicrobial effect. By increasing the amount of extract, the growth rate of the bacteria significantly decreases (p<0.01). The highest non-growth halo in both methods was obtained from the control sample containing chloramphenicol disk on the E. coli (24 mm) and after it, the sample that contain 50% extract on S. aureus had 13.33 non-growth halo in the well method, and in the disc method, the 50% extract of S. aureus had the highest non-growth halo (11 mm). In fact, S. aureus was more susceptible to walnut leaves extract than E. coli in both methods. However, between the two methods, the sensitivity of the wells samples was more than the discontinuation method. The overall result shows that the sample containing 50% walnut leaf extract on S. aureus has the most antimicrobial activity among other samples after the control sample (chloramphenicol disc on E. coli). Manuscript profile
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  4 - شناسایی مواد موثره موجود در عصاره و اسانس گیاه زینتی-دارویی سداب (Ruta graveolens L) و بررسی اثر ضد میکروبی آن‌ها
  فاطمه ترابی داود هاشم آبادی بهزاد کاویانی لیلا اسدپور
  این پژوهش با هدف بررسی مواد موثره موجود در عصاره و اسانس گیاه زینتی-دارویی سداب و بررسی اثر ضد میکروبی آن‌ها انجام شد. بدین‌منظور در آبان ماه 1398 اندام هوایی سداب از زیستگاه طبیعی آن در ارتفاعات چابکسر-استان گیلان جمع‌آوری شد. برگ‌ها و ساقه گیاه در آون الکتریکی با دمای More

  این پژوهش با هدف بررسی مواد موثره موجود در عصاره و اسانس گیاه زینتی-دارویی سداب و بررسی اثر ضد میکروبی آن‌ها انجام شد. بدین‌منظور در آبان ماه 1398 اندام هوایی سداب از زیستگاه طبیعی آن در ارتفاعات چابکسر-استان گیلان جمع‌آوری شد. برگ‌ها و ساقه گیاه در آون الکتریکی با دمای 75 درجه سانتی‌گراد به مدت 24 ساعت خشک و سپس توسط کلونجر عصاره‌ و اسانس گیاه استخراج شد. جهت شناسایی ترکیبات موثره عصاره و اسانس از GC-MS استفاده شد. نتایج نشان داد که Hexadecanoic acid، Isomaturnin، 2-Ethyl-1,3,4,5,6,7,8-heptamethy و12-methoxy-19-norpodocarpa به‌ترتیب بیشترین ترکیب موجود در اسانس برگ، اسانس ساقه، عصاره برگ و عصاره ساقه سداب است. نتایج تست میکروبی به روش دیسک دیفیوژن روی سویه‌های Pseudomonas aeruginosa, Staphylococcus aureus, Acinetobacter baumannii and Escherichia coli نشان داد که عصاره برگ و ساقه سداب بیشترین اثر را در برابر مهار باکتری‌ A. baumannii دارد. بررسی نتایج MIC و MBC نشان داد که باکتری S. aureus با کمترین MIC و MBC حساس‌ترین باکتری در برابر اسانس برگ و ساقه سداب است درحالی‌که A. baumannii با کمترین MBC بیشترین حساسیت را به عصاره برگ سداب نشان داد. بنابراین می‌توان از عصاره و اسانس گیاه سداب به-عنوان یک ماده ضد میکروبی علیه باکتری‌های بیماری‌زای انسانی استفاده نمود. Manuscript profile
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  5 - سنتز مشتقات جدیدی از پیرانوکرومن‌ها و بررسی ویژگی ضد باکتری آن‌ها
  منظربانو اثنی عشری اصفهانی اسماعیل وصالی زهرا رستگار زهرا اسدی
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