Antibiotic Resistance Pattern of Klebsiella Pneumoniae from Patients in Qaem Teaching Hospital, Mashhad University of Medical Sciences

Subject Areas : environmental Health

Ali  Shamsazar ¹ (Department of Biochemistry, Payame Noor University, Tehran Shargh Branch, Tehran, Iran)
Arshid  Yousefi-Avarvand ² (Ph.D Student of Bacteriology, Department of Microbiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran*(Corresponding Author))
Farzad  Khademi ³ (Ph.D Student of Bacteriology, Department of Microbiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran)

Received: 2016-09-23 Accepted : 2016-12-14 Published : 2020-03-20

Keywords: Antibiotic resistance, Modified Disk Diffusion Method, Klebsiella pneumonia,

Abstract :

Klebsiella pneumoniae is a gram-negative, non-motile and capsular gram-negative bacillus. It is one of the most important human pathogens and is an important factor in causing nosocomial infections. The bacterium is inherently resistant to a number of antimicrobial agents. The aim of this study was to evaluate the antibiotic resistance pattern of Klebsiella pneumoniae isolated from patients referred to Ghaem hospital in Mashhad. A total of 274 Klebsiella pneumoniae isolates were collected from April to October 2013 from Qaem teaching hospital of Mashhad University of Medical Sciences. Identification of these isolates was performed using microbiological methods such as hot staining and differential media. Modified disk diffusion method (MDDM) was used to determine the susceptibility of bacteria to the antibiotics amoxicillin, nalidixic acid, cefotaxime, ceftizoxime, ceftriaxone, ceftazidime, ciprofloxazine, imipenem, and nitrofurantoin. In this study 175 isolates (87.63%) were from urine sample and 99 isolates (13.36%) were from different samples (blood, cerebrospinal fluid, ulcer, ascites, and secretions). Resistance of Klebsiella pneumoniae isolates to ciprofloxacin, imipenem, nitrofurantoin and gentamicin antibiotics were 71.46%, 9.56%, 3.39% and 30%, respectively. The results of this study indicate that resistance of Klebsiella pneumoniae isolates to different antibiotics is higher than previous studies. Further studies in this area could help guide us to better understand the antibiotic resistance of these infectious bacteria.

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