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      • Open Access Article

        1 - Influence of low electromagnetic field on photosynthetic apparatus and the amount of soluble sugar content in Lepidium sativum L.
        Elham bagheri Abyaneh Ahmad Majd Sayeh Jafari
        Absract This study was carried out to evaluate the effect of extremely low frequency electromagnetic field (60 Hz) on photosynthesis apparatus and the amount of soluble sugar in Lepidium sativum L. Seeds were divided to dry and wet groups. In terms of wet group, seeds w More
        Absract This study was carried out to evaluate the effect of extremely low frequency electromagnetic field (60 Hz) on photosynthesis apparatus and the amount of soluble sugar in Lepidium sativum L. Seeds were divided to dry and wet groups. In terms of wet group, seeds were soaked in water for 7 and 14 h. Dry and wet seeds were placed in the middle of a horizontally fixed coil and were exposed to extremely low frequency electromagnetic of a magnitude of 3.8 mT, for 30 and 60 minutes. Results demonstrated that the amount of chlorophyll a and b did not show the significant increase and the amount of chlorophyll ratio did not have any significant decrease. In addition, carotenoid, flavonoid content and membrane lipid peroxidation increased significantly and the amount of soluble sugar content declined significantly. Results confirms that the influence of electromagnetic field on carotenoid and flavonoids as well as membrane lipids caused the decrease of photosynthesis efficacy, and consequently caused the decline of the amount of soluble sugar. Manuscript profile
      • Open Access Article

        2 - Investigation on germination and phenolic content of tomato (Solanum lycopersicum) in response to cell phone radiation
        Elham Rajabbeigi Mohammadreza Rousta
        Nowadays, the use of cell phone has increased with the modernization. It has led to the increase in the radiations in the environment which further has effect on the plants and animals including human beings. In this research, the grains of tomato (Solanum lycopersicum) More
        Nowadays, the use of cell phone has increased with the modernization. It has led to the increase in the radiations in the environment which further has effect on the plants and animals including human beings. In this research, the grains of tomato (Solanum lycopersicum) were placed in petridish and the mobile phone set was placed over them and it was activated by calling from another phone. The grains under the treatment for 14 days, were radiated for 10 minutes every day. After the treatment, the effects of cell phone waves on tomato (Solanum lycopersicum) seedlings were investigated and the percentage of germination, radical scavengering capacity, and phenolics were examined in compared to control samples. It was observed that radiations emitted from cell phone show considerable increasing in the germination percentage and lipid peroxidation. Radical scavenging activity (RSA) and protein content were declined in response to cell phone radiations. Increasing of seed germination could be caused by inducing gene expression in response to cell phone radiations. It seems that although the decrease in radical scavenging activity, ROS was increased. It could be led to lipid degradation in compare to control ones. Total phenolics decreased in response to phone radiation. It seems that cell phone radiation can affect cells through the membrane degeneration and the production of reactive oxygen species. Manuscript profile
      • Open Access Article

        3 - The Effect of Salicylic Acid on Some Physiological Reactions of Fenugreek (Trigonella foenum-graecum) Under Salinity Stress
        A. Pasandi Pour H. Farahbakhsh M. Saffari B. Karamat
        This study performed to investigate the effects different concentrations of salicylic acid (0, 5, 10, 15 and 20 µM) on membrane lipid peroxidation of fenugreek under levels of salinity stress (0, 50, 100, 150 and 200 mM) in hydroponic condition. The experiment con More
        This study performed to investigate the effects different concentrations of salicylic acid (0, 5, 10, 15 and 20 µM) on membrane lipid peroxidation of fenugreek under levels of salinity stress (0, 50, 100, 150 and 200 mM) in hydroponic condition. The experiment conducted as factorial, in completely randomized design with three replications. Results showed that in plants treated with salinity, chlorophyll a, chlorophyll b and carotenoid contents were reduced in comparison with control plants. However, the salinity increased MDA, other aldehydes, catalase and peroxidase activity in shoots. Results also indicted that applying salicylic acid, especially with concentration of 10 and 15µM, increased the membrane lipid peroxidation and decreased the membrane stability index as compared to the control plants. Results also showed that using 5µM salicylic acid did not significantly improve the salt stress effects compared to the control plants. However, concentration of 20µM salicylic acid increased stress, and decreased the above mentioned parameters. Manuscript profile
      • Open Access Article

        4 - Effect of Heavy Metals Cu and Pb on Some Growth Characteristics, Proline Content and Lipid Peroxidation in Two Varieties of Mung Bean (Vigna radiate)
        مهدی Kehstegar S.A. Afshar S.F. Nematpour
        Heavy metal contaminations of agricultural fields, due to use of industrial waste water and excessive application of phosphate fertilizers, has become a major problem. Biological stability in soil for long-term, causes these metals accumulate in food chain and thus pote More
        Heavy metal contaminations of agricultural fields, due to use of industrial waste water and excessive application of phosphate fertilizers, has become a major problem. Biological stability in soil for long-term, causes these metals accumulate in food chain and thus potentially affect human health. To investigate the effects of copper and lead on growth parameters, lipid per oxidation, peroline content in two cultivars of mung bean, a factorial experiment was conducted in a completely randomized design. Copper used in three concentrations (0,150, 300 and 400mg/kg) and Pb in four concentration (0, 200, 400, 600mg/kg). After four weeks, plants grown in contaminated soil were collected and shoot height, root length, fresh weight and dry weight of shoot and root; proline content and lipid per oxidation in leaves were measured. The results showed that copper and lead had negative and toxic effect on the mung bean growth features. Generally with increasing concentrations of cu and Pb in soil, growth parameters were decreased. Between the two cultivars (Gohar and Sistan), Sistan was affected more than Gohar. Proline content and lipid peroxidation in treated mung bean were increased. Also Sistan cultivar showed more proline content and lipid per oxidation than Gohar.  Manuscript profile
      • Open Access Article

        5 - Comparison of oxidative stress between Cytauxzoon feli infected and healthy cats
        Kimia Zafari Seyedeh Ommolbanin Ghasemian
        Cytauxzoonosis is an emerging infectious disease that affects feral as well as domestic cats. This disease is caused by apicomplexan protozoan parasites belonging to the genus Cytauxzoon. Evaluation of oxidative stress indicators can clarify the adverse effects of Cytau More
        Cytauxzoonosis is an emerging infectious disease that affects feral as well as domestic cats. This disease is caused by apicomplexan protozoan parasites belonging to the genus Cytauxzoon. Evaluation of oxidative stress indicators can clarify the adverse effects of Cytauxzoon¬ felis parasite on domestic cats. The present study aimed to investigate the prevalence of C. ¬felis parasite in cats of Shahrekord, Iran and to evaluate oxidative stress indicators in cats infected with C. ¬felis and compare it with healthy cats. This experimental study was conducted on blood samples of 100 cats that were referred to Shahrekord Veterinary Laboratory. Blood samples were taken in two separate test tubes for molecular analysis and determination of antioxidant parameters including catalase and lipid peroxidation. After collecting the samples, the PCR test was performed and oxidative stress markers were measured. The results of the present study showed that 3% of examined cats were infected with C. ¬felis, and the level of catalase enzyme in the cats infected with C. ¬felis was reduced by 9% compared to the healthy group, which was statistically significant (p=0.004). Moreover, lipid peroxidation level was significantly increased in cats infected with C.¬ felis compared to the parasite-free group (p=0.021). The findings of the present study showed that the prevalence of C.¬ felis parasite in cats was 3%. A decrease in the level of catalase enzyme and an increase in lipid peroxidation were observed in cats infected with C.¬ felis compared to cats free of the parasite. Manuscript profile
      • Open Access Article

        6 - The effect of different levels of garlic extract administration at various time periods on the extent of serumic lipid peroxidation in laying hens
        saeed rasoulinejad nariman sheykhi hossein hassanpour
        Free radicals are involved in molecular changes and mutation and if they are not inactivated the resultant lipid peroxidation will damage all types of cellular macromolecules such as proteins, carbohydrates, lipids and nucleic acids. Natural diet containing antioxidants More
        Free radicals are involved in molecular changes and mutation and if they are not inactivated the resultant lipid peroxidation will damage all types of cellular macromolecules such as proteins, carbohydrates, lipids and nucleic acids. Natural diet containing antioxidants plays an important role in health, reproduction, performance, safety and growth in poultry. Garlic extract with a high antioxidant effect very useful against free radicals and infective agents. In this study, the effect of concentration of 0.01 and 0.02% of garlic extract in drinking water with periods of 2, 4 and 6 days a week were evaluated on lipid peroxidation. In addition to assessing the best concentration, economic aspects were also considered. So at the end of the third week, the best effect on reducing lipid peroxidation was seen in T3 which had received the concentration of 0.02% of garlic extract, two days a week.  After six weeks of trial, Group T7 which had received the concentration of 0.02% garlic extract for 6 days a week, showed the best effect on reducing lipid peroxidation. Our studies also showed that garlic extract improved mass production in all groups compared to the control group.  Considering the amount of lipid peroxidation at the end of the sixth week, herd performance in six weeks and calculating the average sale price of mass production, minus the cost of feed consumed per group, it is concluded that group T4 which received garlic extract concentration of 0.01% for 4 days a week (Saturday, Monday, Wednesday, Thursday) showed the best option in view of the economic conditions. Manuscript profile
      • Open Access Article

        7 - The Effect of Vitamin E Supplementation on Atherogenic and Lipid Peroxidation Responses Induced by Interval Training in Obese Male Wistar Rats
        Fatemeh Hosseini reza rezaeeshirazi Saeed Ghorbani Abuzar Jorbonian
        Introduction: Obesity causes high disorders as increasing atherogenic index and physical activity is able to control obesity that can possibly increase lipid peroxidation. Therefore, the study was performed to evaluate the effect of vitamin E supplementation on atheroge More
        Introduction: Obesity causes high disorders as increasing atherogenic index and physical activity is able to control obesity that can possibly increase lipid peroxidation. Therefore, the study was performed to evaluate the effect of vitamin E supplementation on atherogenic and lipid peroxidation responses induced by interval training in obese male Wistar rats.Materials and methods: The present study was a laboratory experimental method. Thirty male Wistar rats were randomly selected and divided into four groups: training (TG; N=8), supplementation (SG; N=8), training with supplementation (TSG; N=8), and Control (CG; N=8). The interval training consisted of 30 minutes running on treadmill per day, 5 days a week, for 8 weeks. Vitamin E supplementation was also available daily with 300 mg/g of body weight along with drinking water in SG. Finding: The finding revealed a significant decrease between TSG compared with CG in MDA (p=0.010), AIP (p=0.001), TC (p=0.001), TG (p=0.001) and LDL-C (p=0.001) variables. Also, MDA variable a significant decrease between TSG compared with TG (p=0.001). A significant decrease between TG compared with CG and SG in AIP (p=0.031), TC (p=0.024), TG (p=0.012) and LDL-C (p=0.010) variables were observed; while HDL-C variable showed a significant increase between TSG compared with CG (p=0.024) and TG compared with CG (p=0.031) (p≤0.05).Discussion and Conclusion: Vitamin E supplementation can improve atherogenic index and by increasing of antioxidant system causes reduce exercise-induced lipid peroxidation in obese rats; while the positive effects of improving body composition and controlling obesity were achieved to exercise. Manuscript profile
      • Open Access Article

        8 - Investigation on the effect of NaCl on the activity of peroxidase and peroxidation of lipids in roots of two sensitive and tolerant cultivars of wheat (Triticum aestivum L.)
        فائزه Ghanati, Elham Nayyeri Torshizi
        In this paper, the effect of NaCl on the activity of soluble (SPO) and wall bound peroxidases (IPO, CPO) as well as the level of lipid peroxidation in roots of two cultivars of wheat (Triticum aestivum L.) were studied. Two wheat cultivars, Mahooti and Alamoot were sele More
        In this paper, the effect of NaCl on the activity of soluble (SPO) and wall bound peroxidases (IPO, CPO) as well as the level of lipid peroxidation in roots of two cultivars of wheat (Triticum aestivum L.) were studied. Two wheat cultivars, Mahooti and Alamoot were selected as salt-tolerant and salt-sensitive cultivars, respectively. The plants were treated with 300mM NaCl for 24, 48 and 96 hours. The content of lignin and the ratio of CWP/FW were studied during 96 hours of treatment as well. Salt treatment increased the rate of lipid peroxidation and enhanced the activity of wall bound peroxidases, particularly in roots of Mahooti. These phenomena were also associated with an increase in the content of lignin in the walls. The activity of soluble peroxidase was also stimulated which helps to more scavenging of peroxide radicals produced by NaCl treatment. Meanwhile, the results suggest that in salt-tolerant wheat cultivar, NaCl treatment accelerated aging process. Manuscript profile
      • Open Access Article

        9 - Study of the effect of the chitosan and chitosan nanoparticles on some physiological and phytochemical features of Nigella sativa L.
        Farahnaz Mahdipour sara saadatmand Alireza Iranbakhsh Bahare Norozi zahra Oraghi Ardebili
        Black cumin (Nigella sativa L.) from the Ranunculaceae family is considered one of the best sources of natural antioxidants. Due to the positive effect of chitosan on various medicinal plants, in this study we investigated the vegetative and chemical performance of this More
        Black cumin (Nigella sativa L.) from the Ranunculaceae family is considered one of the best sources of natural antioxidants. Due to the positive effect of chitosan on various medicinal plants, in this study we investigated the vegetative and chemical performance of this plant under the treatment of chitosan nanoparticles. Experimental factors included solubilization of chitosan and its nanoparticles with concentrations of 0.01, 0.05, 0.2, 1, 4 (pH 5) percent. Assays were performed on the seed and leaf extracts of the treated plant at Razi Laboratory of Azad University, Science and Research Branch of Tehran in 2021. Extraction was done by cold pressing method. Some traits such as germination (number, percentage, index and germination rate), growth parameters (radicle and plumule length, fresh radicle and plumule weight and radicle and plumule dry weight), pigments, total leaf phenol content (Folin-Ciocalteau) total leaf flavonoids (aluminum chloride colorimetric assay), leaf antioxidant activity (DPPH), leaf membrane lipid peroxidation (MDA concentration) and soluble protein content Seeds and leaves (Bradford) were evaluated. The experiment was conducted as a completely randomized design with 3 replications and the comparison of data means was performed using Duncan's test at a probability level of 5%. The results showed that the treatment percentages had a significant effect on all evaluated traits (except the fresh weight of the radicle). Treatment of 1% and 0.01% of chitosan nanoparticles increased the growth and germination parameters. In addition, the amount of phenol, flavonoids and antioxidant activity increased compared to the control showed that the highest increase was observed in concentrations of 1% and 0.01% chitosan nanoparticles. The maximum increase in the amount of pigments was due to the concentration of 1% and 0.2% of chitosan nanoparticles. Both treatments at a concentration of 1% reduced the amount of MDA compared to the control. The amount of total protein in leaves and seeds decreased under the influence of the treatments .In general, it was concluded that the treatment of chitosan nanoparticles as a bio stimulant has a positive effect on improving the quality characteristics of black seed and they are also suggested as a suitable stimulus to increase growth. Manuscript profile
      • Open Access Article

        10 - تاثیر عصاره آزاد و ریزپوشانی شده گیاه رزماری بر ویژگی های کیفی و زمان ماندگاری گوشت طی دوره نگهداری
        سیده سلیمه رشیدایی آبندانسری پیمان آریایی مهدی چرمچیان لنگرودی
      • Open Access Article

        11 - The effect of methanol extract of Lippia citrodoria in the prevention and control of IBD induced by acetic acid in mice
        maryam teimouri Fariba Khosravinejad
        Inflammatory bowel disease (IBD) is a chronic condition of the intestine with unknown etiology involving multiple immune, genetic and environmental factors. We were interested to examine the effect of extract from Lippia citrodoria, a medicine plant in prevention and co More
        Inflammatory bowel disease (IBD) is a chronic condition of the intestine with unknown etiology involving multiple immune, genetic and environmental factors. We were interested to examine the effect of extract from Lippia citrodoria, a medicine plant in prevention and control of experimental mouse IBD. L.citrodoria was administered (50, 150.200 mg/kg) through drinking water to IBD mice (induced by intrarectal administration of acid-induced). Prednisolone was used as the standard drug for comparison. Biochemical, macroscopic and microscopic examination of colon were performed. Biochemical evaluation of inflamed colon was done using assay of myeloperoxidase (MPO) activity and thiobarbituric acid reaction substances(TBARS) concentration as indicators of free radical activity and cells lipid peroxidation. Results indicated that the activity of MPO and lipid peroxidation products (TBARS) increased in acetic acid-treated group while recovered by pretreatment of animals with L.citrodoria (50,150,200 mg/kg) and prednisolone. L.citrodoria (50-100mg/kg) and prednisolone. L.citrodoria (50-200 mg/kg) and prednisolone-treated groups showed significantly lower score values of macroscopic and microscopic characters when compared to the acetic acid – treated group. The benefical effect of L.citrodoria ( 200mg/kg) was comparable to that of prednisolone.It is concludes that the antioxidant, antimicrobial, and anti-inflammatory potentials of L.citrodoria might be the mechanisms by which this extract protects animals against experimentally induced IBD. Proper clinical investigation should be carried out to confirm the activity in human Manuscript profile
      • Open Access Article

        12 - Study the effect of salinity stress on antioxidant enzymes activity and indicators oxidative stress in weeds Redroot Pigweed (Amaranthus retroflexus L.), Bindweed (Convolvulus arvensis L.) and Russian Knapweed (Acroptilon repens L.)
        V. Enaiati E. Esfandiari A. Pourmahammad K. Mohammadnia اصغر Rahimi
        Waiver of noticeable weeds injuries on crop yield, so far has not been studied the effects of salinity on behavior physiological and biochemical. In order to weeds redroot pigweed, Bindweed and Russian knapweed choice and the effects of three levels of salinity control More
        Waiver of noticeable weeds injuries on crop yield, so far has not been studied the effects of salinity on behavior physiological and biochemical. In order to weeds redroot pigweed, Bindweed and Russian knapweed choice and the effects of three levels of salinity control (0), 50 and 100 mM, in a randomized complete block design with 4 replications in a greenhouse were studied. The results showed that levels of salinity only on dry weight was not significant in the bindweed. Moreover the superoxide dismutase (SOD) activity in salinity decreased in Redroot Pigweed and Russian Knapweed weeds, but its activity was not affected in bindweed of salinity. Also, the results of assay activity removal enzymes of hydrogen peroxide showed that this enzymes did not affect only the bindweed of salinity. Whereas in Russian knapweed the activity this enzyme was significantly reduced. In Redroot pigweed also only enzyme Ascorbate peroxidase (APX) activity was reduced by salinity. In studied weeds the salinity had not effect activity of the glutathione S- transferase (GST) enzyme. On the other hand, the amount of hydrogen peroxide (H2O2) in the three studied weeds significantly increased on salinity of 100 mM. But increase the rate of lipid peroxidation at effect salinity observed only in Russian knapweed and redroot pigweed. Based on the results due to the lack of significant changes in antioxidant enzymes at bindweed, this plant is tolerant to salinity levels investigated compared to Russian knapweed and redroot pigweed. Manuscript profile
      • Open Access Article

        13 - The Effects of Symbiotic on Oxidative Stress Reduction in Broilers with Pulmonary Hypertension Syndrome
        Hamed Zarei Abbass Fereydoon Kolahi
        Pulmonary Hypertension Syndrome (PHS), characterized by increased lung pressure in arteries, dilatation, and hypertrophy of the right ventricle, is correlated with the high cardiac output and enhanced blood pressure in the lungs to more blood oxygenation, which ultimate More
        Pulmonary Hypertension Syndrome (PHS), characterized by increased lung pressure in arteries, dilatation, and hypertrophy of the right ventricle, is correlated with the high cardiac output and enhanced blood pressure in the lungs to more blood oxygenation, which ultimately leads to ascites. The accumulation of free radicals and increased oxidative stress result in both lipid peroxidation, as well as the increased levels of heterophil to lymphocytes. By having antioxidant properties, the synbiotic can significantly reduce oxidative stress. Therefore, in the present study, we aimed to investigate the therapeutic effects of synbiotic on pulmonary hypertension in broilers. Groups were categorized into; Sham (broilers with a basic diet in the whole period of the test), Control (recipient of basic diet+ triiodothyronine hormone (T3) at 1.5 mg/kg doses added to their diet from seven days of age), Treatment with synbiotic (birds with basic diet + T3 at 1.5 mg/kg dose added to their diet from seven days of age + synbiotic (0.1% dose) from day one). At 35 and 49 days of age, 9 birds from each group (3 broilers from each replicate) were randomly selected, and after getting the blood samples, heterophil to lymphocyte rate and lipid peroxidation was assessed. To evaluate pulmonary hypertension, cardiac weight index was also reported due to the right ventricular weight to the total weight of both ventricles (RV/TV). RV/TV rate at all ages was decreased in the synbiotic broilers; however, this was statistically significant at 49 days of age (p ≤ 0.05). Compared to the control group, lipid peroxidation levels in synbiotic group at all ages were reduced which was significant at 35 and 49 days of age (p < 0.05). Furthermore, Heterophil to lymphocytes rates was decreased in the synbiotic group compared to the control group in all ages, but this decrease was more significant at 49 days of age (p < 0.05). Regarding the obtained results, symbiotic, for their antioxidant properties, can significantly reduce the amount of oxidative stress result from pulmonary hypertension syndrome. Manuscript profile
      • Open Access Article

        14 - The effect of salicylic acid on some morphological and physiological traits under salinity stress (Catharanthus roseus)
        Samaneh Abdolmohammadi Jalal Omidi
        Abstract: Considering the increasing trend of salinity development and lack of desirable land for agriculture in the world, the use of saline resistant species or the use of compounds that reduce the effects of salinity stress and induction of resistance to stress in pl More
        Abstract: Considering the increasing trend of salinity development and lack of desirable land for agriculture in the world, the use of saline resistant species or the use of compounds that reduce the effects of salinity stress and induction of resistance to stress in plants is very important. Salicylic acid is one of the beneficial compounds for plants, which plays an important role in the resistance of plants to environmental stresses, including salinity stress. To evaluate the potential beneficial effects of salicylic acid on some of the physiological and morphological parameters of an experiment, a factorial experiment was conducted in a completely randomized design with two factors: salinity including sodium chloride at 0, 100 and 150 mM and salicylic acid at two levels 0 and 1 mM with four repeats. At the end of experiment, vegetative traits, chlorophyll a, b, total, carotenoids, starch, sucrose and total protein content were measured after 28 days of treatment. The results of salinity and salicylic acid interaction showed that salinity significantly reduced the weight and dry weight of the shoot, root dry weight and root, root penetration depth, and the height of the forage plant. Salinity of 150 mM significantly reduced the amount of chlorophyll a and b. In addition, the amount of sucrose and starch and protein decreased by salinity. Treatments with salicylic acid increased vegetative traits, sugars, chlorophyll, carotenoids and protein under salinity stress significantly. Manuscript profile
      • Open Access Article

        15 - Effect of seed deterioration on germination, biochemical and enzymatic activity of buckwheat
        آرزو پراور Saeideh Maleki Farahani Shokofeh Gholami
         To determine the effect of seed deterioration on germination, biochemical and enzymatic activity of buckwheat, an experiment was conducted as factorial based on completely randomized design in Seed Science and Technology Laboratory of Shahed University, Iran. The More
         To determine the effect of seed deterioration on germination, biochemical and enzymatic activity of buckwheat, an experiment was conducted as factorial based on completely randomized design in Seed Science and Technology Laboratory of Shahed University, Iran. The treatments consisted of ageing duration (non-aging, 2, 4 and 7 days) and seed moisture content (10, 20 and 30%). Parameters such as germination percentage, mean germination time, electrical conductivity, lipid peroxidation and catalase activity were measured. The effect of aging duration, seed moisture content and their interaction on traits were significant. Germination percentage, mean germination time, electrical conductivity, lipid peroxidation, and catalase enzyme activity were significantly affected by aging duration and seed moisture content. The highest germination percentage and catalase enzyme activity in aged seeds was observed during two days with 20% moisture content. Mean germination time, electrical conductivity and lipid peroxidation in aged seeds for seven days was in the highest level in seeds with 30% moisture content. Seed moisture content and aging duration increased the mechanisms involved in seed aging and cell membrane phospholipids affected by deterioration, which increased the electrical conductivity and lipid peroxidation and reduced germination and catalase enzyme activity. To prevent buckwheat seed deterioration in long and short term storage, it is recommended that seed moisture content is kept about 10%. Manuscript profile