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Open Access Article
1 - اثر محلولپاشی اسیدسالیسیلیک، سیلیکات پتاسیم و الیسیتور قارچی در شرایط تنش شوری بر گیاه همیشه بهار (Calendula officinalis L.)
نرگس حسینی فر وحید عبدوسی وحید زرین نیا -
Open Access Article
2 - Effect of Chitosan on Physiological and Morphological Traits of Lemon Verbena (Lippia citriodora L.) under in Vitro and Field Conditions
Hassan NourafcanChitosan is one of the bio-elicitors that cause physiological and morphological changes in plants. To this end, two separate experiments were carried out to investigate the effect of chitosan on morphological and physiological traits of lemon verbena under in vitro and MoreChitosan is one of the bio-elicitors that cause physiological and morphological changes in plants. To this end, two separate experiments were carried out to investigate the effect of chitosan on morphological and physiological traits of lemon verbena under in vitro and field conditions. 12.5, 25, 50 and 100 ppm concentrations of chitosan were added to MS culture medium and their effects on lemon verbena growth indices were evaluated. The results showed that chitosan had a significant effect on all traits, except for the normal plantlet percentage, lateral shoots and chlorotic leaf numbers evaluated. Increasing chitosan concentrations up to 50 and 100 ppm under in vitro condition increased longest shoot length, chlorophyll content and plantlet length in lemon verbena explants but it reduced node numbers of the longest shoot when all concentrations of chitosan were used. Increasing the effect of chitosan on rooting percentage and root numbers were positive and reached the highest levels at concentrations of 50 and 100 ppm. It was also found that the shoot fresh weight, the leaf number and longest root have a positive effect on chitosan and had a better effect on concentrations less than 100 ppm. However, the use of chitosan showed irregular changes in the amount of citral. The effect of chitosan with 12.5, 25, 50 and 100 ppm concentrations were evaluated on growth indices of lemon verbena under field condition. The positive effect of chitosan concentration of 50 ppm was observed for inflorescence number, inflorescence length and leaf fresh weight. Because of positive effects of chitosan concentrations of 50 and 100 ppm, on most growth characteristics of lemon verbena in field and tissue culture conditions their applications could be recommended in plantations of this tree. Manuscript profile -
Open Access Article
3 - Effect of Salicylic Acid on Morpho-physiological Characteristics of Lemon verbena (Lippia citriodora) in In Vitro and Field Conditions
Hassan NourafcanSalicylic acid (SA) is a natural phenolic and a hormone like compound, and its different concentrations and treatment duration cause many actions and reactions in plants. Thus, two experiments were conducted to evaluate the effect of salicylic acid on morphological and MoreSalicylic acid (SA) is a natural phenolic and a hormone like compound, and its different concentrations and treatment duration cause many actions and reactions in plants. Thus, two experiments were conducted to evaluate the effect of salicylic acid on morphological and physiological characteristics of lemon verbena as an ornamental and medicinal plant in in vitro and field conditions at Miyaneh, Iran. In tissue culture conditions, five concentrations of SA including 0, 2.5, 5, 10 and 20 μM were added to MS culture medium and their effects on lemon verbena growth indices were evaluated. Increasing SA concentrations caused increment in chlorotic leaf number but decrement in normal plantlet percentage and lateral shoots number in lemon verbena explants. However, in low concentrations of SA, longest shoot length, leaf number and chlorophyll content were increased. Although all concentrations of SA improved shoot fresh weight in lemon verbena explants. Under field condition, the effect of salicylic acid at 2.5, 5, 10 and 20 μM concentrations, non spray and spray with distilled water, were evaluated on morphological and physiological traits of lemon verbena. Salicylic acid affected significantly leaf width, inflorescence number, fresh inflorescence and leaf weights and the longest inflorescence length. The negative effect of SA application on leaf width and number and fresh weight of inflorescence was observed. But leaf fresh weight and inflorescence length improved especially in low SA concentrations. Therefore, application of low SA concentrations, because of their enhancing potential effect on vegetative growth of lemon verbena both in field and tissue culture conditions can be recommended. Manuscript profile -
Open Access Article
4 - Effects of foliar application of salicylic acid and proline on the growth traits and the essential oil content of Dracocephalum kotschyi Boiss. under water deficit conditions
Azam Shaykh Samani Abdollah Ghasemi Pirbalouti Mehrab Yadegari Faezeh RajabzadehIn the drought conditions of the last few decades, the use of novel scientific methods to increase the growth and production of effective substances of the medicinal and aromatic plants has been essential. This study was done to investigate the effect of foliar applicat MoreIn the drought conditions of the last few decades, the use of novel scientific methods to increase the growth and production of effective substances of the medicinal and aromatic plants has been essential. This study was done to investigate the effect of foliar application of salicylic acid and proline on the growth traits and essential oil content of Dracocephalum kotschyi Boiss. (Lamiaceae family) under water deficit conditions at the Research Farm of the Research Center of Agricultural and Natural Resources of Chaharmahal va Bakhtiari Province, Iran during the two crop years of 2017-2019. The experiment was performed as a factorial in a CRBD with three replications. Experimental factors included irrigation treatment at two levels of normal irrigation (non-stress, irrigation after draining 30% of F.C.) and deficit irrigation regime (irrigation after draining 50% of F.C.), and three levels of foliar application (control, salicylic acid 1 mM, and proline 1 mM). The essential oil from the aerial parts of the herb at early flowering stage was extracted by water distillation. According to the results, the highest content of chlorophyll a, b, and total (0.96, 0.45, and 1.39 mg/g, respectively) were observed in water deficit irrigation treatment. Also, application of water deficit stress increased the proline content by 70% compared to the control while it did not show a significant effect on the morphological traits of plant height, stem diameter, and plant dry weight. Salicylic acid as a defense factor reduced the negative effects of deficit irrigation conditions on the essential oil, proline, and chlorophylls a, b, and total contents. The highest and lowest contents of essential oils were obtained in foliar-application of proline (1.12 mL/100 g dry matter) and control (0.84 mL/100 g dry matter), respectively. In conclusion, the foliar-spraying of salicylic acid in conditions of deficit irrigation is recommended to reduce the negative effects of stress and increase the content of D. kotschyi essential oil. Manuscript profile -
Open Access Article
5 - Evaluation the effect of elicitors on antioxidant properties and mycelial secondary metabolites of Stereum hirsutum, Hyphodontia paradoxa and Arthrinium arundinis from Golestan province
lazemi golaem vahide payamnoor jamile nazari omran alishahThe aim of this study was to evaluate the effect of elicitors on antioxidant properties and mycelial secondary metabolites of Arthrinium arundinis, Lichen symbiont, and Hyphodontia paradoxa and Stereum hirsutum hosted by Betula pendula . In the summer of 2016 preliminar MoreThe aim of this study was to evaluate the effect of elicitors on antioxidant properties and mycelial secondary metabolites of Arthrinium arundinis, Lichen symbiont, and Hyphodontia paradoxa and Stereum hirsutum hosted by Betula pendula . In the summer of 2016 preliminary sampling was performed from tree barks of Siah-Marzkouh, Golestan province, from an altitude of 2400 m above sea level. Then, fungal purification was achieved. In 2018, biennial mycelial reserves were used to investigate changes in secondary compounds. The following treatments were applied as elicitor: sodium nitrate, nanofibers cellulose and magnesium sulphate. Initial evaluation of triterpenoids was performed by HPLC using standard betulin and betulinic acid, and LC-MS was used to confirm the corresponding peak as triterpenoid. Antioxidant properties in mycelia of these fungi were studied by DPPH method. Based on the results, the highest peak of triterpenoids which are derivatives of betulin was in H. paradoxa treated by nanofibers cellulose, while in S. hirsutum it was by sodium nitrate (6.5 and 6.37 times in comparison to control, respectively). In A. arundinis, elicitors had no effect on triterpenoids amount and significantly reduced it. The antioxidant activity of H. paradoxa in the control treatment was 83.1% and the induction of elicitors had the opposite effect on this activity. The antioxidant activity of A. arundinis decreased from 82.05 to 87.12% with magnesium sulfate elicitor, meanwhile by adding sodium nitrate to the medium it increased from 24.62 to 86.04%. Manuscript profile -
Open Access Article
6 - کاربرد الیسیتورها برای افزایش تولید متابولیت های ثانویه در سوسپانسیونهای کشت سلول و اندام گیاهی
مریم محمدی فارسانی عبدالله قاسمی پیربلوطیدر زیست فناوری همواره به بررسی مسیرهای جایگزین برای تولید ترکیبات طبیعی توجه میشود. سیستمهای کشت سلول و اندام گیاهی برای تولید متابولیت های ثانویه ای که اهمیت تجاری در صنایع غذایی و دارویی دارند قابل جایگزینی میباشند. با این حال، تقریباً تعداد کمی از محیطهای کشت این Moreدر زیست فناوری همواره به بررسی مسیرهای جایگزین برای تولید ترکیبات طبیعی توجه میشود. سیستمهای کشت سلول و اندام گیاهی برای تولید متابولیت های ثانویه ای که اهمیت تجاری در صنایع غذایی و دارویی دارند قابل جایگزینی میباشند. با این حال، تقریباً تعداد کمی از محیطهای کشت این ترکیبات را سنتز میکنند و میزان این سنتز و مدت زمانی که صرف میکند قابل مقایسه با استفاده از یک گیاه کامل میباشد. روشهایی شامل تغییر محیط کشت (افزودن مواد مغذی و هورمونی) و شرایط محیطی (تغییرات دما، pH و اسمزی) و همین طور از مواد استخراجی و ترکیبی نیز در این راهبردها استفاده می شده است. امروزه از دستکاری ژنتیکی مسیرهای بیوسنتتیک با استفاده از مهندسی متابولیکی که امروزه برای بهبود تولیدات متابولیت های مورد نیاز تکنیکی قدرتمند شده است استفاده میشود. نتایج مطالعات انجام شده نشان میدهد که هم الیسیتورهای زیستی و هم غیر زیستی سنتز متابولیتهای ثانویه را در محیط کشت سلولهای گیاهی افزایش میدهند. Manuscript profile -
Open Access Article
7 - Investigating the effect of Humic acid treatment on antioxidant capacity, phenol and photosynthetic pigments of the medicinal plant Artemisia annua L. under salt stress
A. Mohammadinia Hossein Moradi V. AkbarpoorSalinity is one of the most important abiotic stresses that, depending on the type of plant,reduces the ability to produce crops.In order to investigate the effect of different concentrations of Humic acid on decreasing adverse effects of salinity in the Artemisia annua MoreSalinity is one of the most important abiotic stresses that, depending on the type of plant,reduces the ability to produce crops.In order to investigate the effect of different concentrations of Humic acid on decreasing adverse effects of salinity in the Artemisia annua plant,a field pot experiment with three replications was conducted in the form of a factorial randomized complete blocks design at Sari University of Agricultural Sciences and Natural Resources.The experimental treatments included three levels of Humic acid(0,150and300 mg/l)and three levels of salinity(laboratory NaCl:0,4 and 6 g/l).After applying the treatments and during the vegetative growth stage,samples were collected from the aerial parts, and the antioxidant capacity(DPPH),the amount of phenol(folinciocalto),flavonoid(aluminum chloride),photosynthetic pigment, proline and plant biomass were analyzed.The results showed that the application of Humic acid(300 mg/litre)and salinity(especially at 6 g/liter)respectively with the average 0.13, 0.39, 0.61, 6/18(mg/g fresh weight)and 20.92(μg/ml)could effectively increase the Artemisia annua photosynthetic pigments.The use of humic acid with a concentration of300 mg/liter and salinity(especially at a concentration of 4 g/liter)with an average of 2.39(mg of gallic acid per100g)and0.115(mg of quercetin per100g),respectively,in increasing the amount of phenol And flavonoid was effective.The maximum biomass of aerial organs was observed in the treatment of 150 mg/liter of humic acid and no application of salt with an average of15.5%.Therefore, Humic acid can partially compensate for the destructive effects of salinity in Artemisia annua.Using abiotic stresses(saline stress)and organic fertilizers(Humic acid)and combining these treatments at different concentrations could improve some morphological and biochemical characteristics in Artemisia annua, depending on the intended purpose. Manuscript profile
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