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Open Access Article
1 - Diagnostic Value of Milk Enzymatic Markers Lactate Dehydrogenase and Alkaline Phosphatase for Subclinical Mastitis in Dairy Cattle
سعید Akbari Aliabad S. O. Ghasemian S. Z. PeighambarzadehMastitis could be diagnosed by diverse methods, including physical, clinical, and laboratory tests. The present study aimed to evaluate the accuracy of subclinical mastitis diagnosis using enzymatic diagnostic tests for milk lactate dehydrogenase (LDH) and alkaline phos MoreMastitis could be diagnosed by diverse methods, including physical, clinical, and laboratory tests. The present study aimed to evaluate the accuracy of subclinical mastitis diagnosis using enzymatic diagnostic tests for milk lactate dehydrogenase (LDH) and alkaline phosphatase (ALP). This study was performed on 90 Holstein cows selected randomly out of high- and low-yielding dairy cattle. According to the result of the California Mastitis Test, two milk samples were obtained from the intended udder quarters based on the principles of the National Mastitis Council. One specimen was used for microbial culture and the other one was utilized for somatic cell count (SCC), as well as milk LDH and ALP tests by diagnostic kits. Considering microbial culture as the gold standard for subclinical mastitis diagnosis, the sensitivity of SCC, ALP, and LDH tests was 87%, 59.3%, and 70.4%, respectively. Moreover, the specificity of these tests was 77.8%, 80.6%, and 77.8%, respectively. Our findings revealed a strong correlation between SCC, LDH, and ALP. It was observed that an increase in SCC was accompanied by augmented LDH response. According to the results of the present study, SCC was the most efficient test for diagnosing subclinical mastitis in dairy cows, followed by LDH and ALP. Manuscript profile -
Open Access Article
2 - بررسی فصلی میزان سرمی کلسیم، فسفر و آلکالین فسفاتاز در گوسفندان منطقه تبریز
امیرپرویز رضایی صابر حجت اله کلانتری ورقه -
Open Access Article
3 - Seasonal evaluation of serumal Ca, P and ALP of slaughtered cattle in Tabriz abattoir
A.P Rezaei-Saber مجید Khoylou یوسف Nouri Khashandarag Increasing knowledge of metabolic disorders and prevention of any prduction decrease is beneficial in animal breeding. Problems related to calcium and phosphorus deficiency in high producing animals are among the most important metabolic disorders wi More Increasing knowledge of metabolic disorders and prevention of any prduction decrease is beneficial in animal breeding. Problems related to calcium and phosphorus deficiency in high producing animals are among the most important metabolic disorders with disease such as osteomalacia, rickets, milk fever, pos parturition hemoglobinuria and reproductive disorders named as the metabolic complications. This study was conducted on non pregnant crossbreed dairy cattle. In the middle of each season with daily referral to the abattoir, blood samples were collected in veneject tubes and after transfer to the laboratory, the serumic levels of Ca, P and ALP were measured by spectrophotometry. One hundred samples for each season and a total of 400 samples were studied. The mean serumic levels of Ca, P and ALP were 8.83±0.02 mg/dl, 3.88±0.07 mg/dl, 163.4±0.8 Iu/lit; 9.05±0.03mg/dl, 4.19±0.01mg/dl, 240.7±1.2 Iu/lit; 9.03±0.07 mg/dl, 5.33±0.02mg/dl, 185.06±2.3 Iu/lit and 9.33±0.09 mg/dl, 5.74±0.08 mg/dl, 230.03±7.4 Iu/lit, in the spring, summer, autumn and winter respectively. Considering the normal Ca, P and ALP values in cattle, 6.3% and 83% of cases had Ca and P deficiency respectively in the spring, 80% of cases had subclinical phosphorus deficiency in the summer, 7.3% of cases had subclinical phosphorus deficiency in the autumn and 9.45% and 7.36% of cases had Ca deficiency and ALP elevation respectively in the winter. The significant decrease in Ca and P levels in the spring and P levels in the summer (p<0.05) is probably to due to the use of green forage with high carotene levels which interferes with Vit D absorption while Ca reduction in the autumn and winter can be due to the cold weather, hand made diets and low sunshine. The increase in ALP levels also correlates with Ca deficiency in the winter. Furthermore reduction of the serumic levels of Ca and P in the age of 4-5 years was significant (p<0/01) compared with the other ages which could be due to high production at this period. Manuscript profile -
Open Access Article
4 - The effect of phosphate solublizing bacteria as a novel probiotic on some biochemical parameters, carcass characteristics and performance of broiler chicks
مهدی Ghaderi Jouybari M.A Malbobi مهرداد Irani وحید Rezaei Pour مهدی Mohammad Zadeh NagharchiIn this study, the effect of Pseudomonas Putida and Pantoa Agglomerans which belong to phosphate solublizing bacteria was investigated as a probiotic on serum concentrations of phosphorous and alkaline phosphatase, some carcass characteristics and performance of broiler MoreIn this study, the effect of Pseudomonas Putida and Pantoa Agglomerans which belong to phosphate solublizing bacteria was investigated as a probiotic on serum concentrations of phosphorous and alkaline phosphatase, some carcass characteristics and performance of broiler chicks. These bacteria, which were isolated with screening soil samples collected from various regions of Iran, were mixed with the diet. The experiment was conducted on a completely randomized design and a 2×4 factorial arrangement. The factors consisted of 4 probiotic levels (1-no probiotic use, 2- probiotic use through out rearing, 3- probiotic use at the start of rearing and during growth period, 4- probiotic use at the end of the rearing period) and 2 phosphorus levels (1-available, 2-total). In this experiment, 8 treatments, 4 replicates with 20 chicks at each replicate and a total of 640 day old chicks of ROSS strain were used. Comparison of means indicated that the probiotic had significantly increased body weight, feed intake and feed conversion ratio (p<0.05). The novel probiotic had increased carcass yield and serum phosphorus (p<0.05). On the other hand, a significant decreased abdominal fat and serum alkaline phosphatase was observed (p>0.05). Also there was no significant effect on breast and thigh weight (p<0.05). The Results of this study indicated that the employed bacteria increase the performance and bioavailability of phosphorous. Manuscript profile -
Open Access Article
5 - A survey on serumic zinc levels of sheep in Miandoab Province in 2008
A.P Rezaei Saber یوسف Davoodi علی HassanpourDue to complexity of Zinc metabolism in the body, there is much interest to study this element. Every phase of growth and development requires Zinc. Zinc deficiency leads to many complications including reproduction and fertility disorders, weakness of the immune system MoreDue to complexity of Zinc metabolism in the body, there is much interest to study this element. Every phase of growth and development requires Zinc. Zinc deficiency leads to many complications including reproduction and fertility disorders, weakness of the immune system and occurrence of secondary infections. In this study, blood samples were obtained from the jugular vein of 200 apparently healthy sheep of Miandoab province using venoject tubes in each season of the year (a total of 800 samples) following age and gender determination. Based on the one-way analysis of variance, there was a significant difference (p<0.01) in serumic levels of Zinc and alkaline phosophatase between difference seasons indicating severe reduction of Zinc levels and increase in Alkalin phosophatase levels during the winter which could be due to consumption of hand fed dried forage containing dust and soil. Also in this study, there was a significant difference (p<0.01) in serumic levels of Zinc and Alkalin phosphatase between different ages of the animals. There was a significant difference in serumic levels of Zinc and Alkalin phosphatase between male and female sheep based on t-Test (p<0.01). Manuscript profile -
Open Access Article
6 - اثرات کود سدیمی و آفلاتوکسین B1 بر وزن بدن جوجههای گوشتی
م. اسکالیکا ب. کورنکوواهدف از این مطالعه بررسی اثر محافظتی کود طبیعی جاذب سدیم (HuNa) در جیره بر پیشگیری از اثر سمیت آفلاتوکسین B1 (AFB1) در جوجه­های گوشتی بود. 72 جوجه گوشتی آمیخته هایبرو به صورت تصادفی در 4 گروه تقسیم شدند: گروه کنترل (G1) و سه گروه آزمایشی (G2 تا G4) با افزودن HuNa (G2 Moreهدف از این مطالعه بررسی اثر محافظتی کود طبیعی جاذب سدیم (HuNa) در جیره بر پیشگیری از اثر سمیت آفلاتوکسین B1 (AFB1) در جوجه­های گوشتی بود. 72 جوجه گوشتی آمیخته هایبرو به صورت تصادفی در 4 گروه تقسیم شدند: گروه کنترل (G1) و سه گروه آزمایشی (G2 تا G4) با افزودن HuNa (G2)، تنها AFB1 (G3) و HuNa و AFB1 (G4). HuNa به مخلوط خوراک کامل برای جوجه­ها افزوده شد (با نسبت 1 گرم HuNa در هر 100 گرم مخلوط خوراک). AFB1 نیز با غلظت 25 میکروگرم در هر کیلوگرم وزن بدن افزوده گردید. پارامترهای ارزیابی شده عبارت از وزن بدن و سطوح سرمی آلکالین فسفاتاز (ALP) بود. AFB1 و HuNa برای جوجه­های گوشتی از 28 روزگی به کار گرفته شدند. کاهش معنی­دار آماری در فعالیت ALP (05/0>P) در گروه G3 در مقایسه با گروه G1 جوجه­های گوشتی از 42 تا 56 روزگی به بعد مشاهده شد. ولی افزایش معنی­دار آماری در فعالیت ALP (05/0>P) در گروه G4 در مقایسه با گروه G1 مشاهده گردید. وزن بدن جوجه­های گوشتی پس از قرار گرفتن در معرض AFB1 (G3) در مقایسه با شاهد (G1) حدود 180 گرم پایین­تر بود. همچنین افزودن HuNa سبب افزایش وزن بدن جوجه­های گوشتی تا حدود 67/26 گرم در انتهای آزمایش در مقایسه با گروه شاهد گردید. افزایش معنی­دار آماری (05/0>P) در وزن بدن برای گروه G3 و G4 در سن 56 روزگی در مقایسه با سن 28 روزگی ثبت گردید. نتایج این آزمایش نشان می­دهند که HuNa اثر مثبتی بر رشد جوجه­های گوشتی داشته و می­تواند یک افزودنی طبیعی مناسب برای رشد جوجه­های گوشتی در برابر اثرات زیان­آور آفلاتوکسین­ها باشد. Manuscript profile -
Open Access Article
7 - The Study on the Activities of Digestive Enzymes, Amylase and Alkalyn Phosphatase, in Kutum Larvae Fed by Artemia nauplii
فاطمه حسن تبار حسین اورجی ابوالقاسم اسماعیلی سیده صدیقه باباییThe aim of this study was investigation of growth rate, survival and activity of digestive enzymes (andalpha;-amylase and alkaline phosphatase) in fish larvae fed Artemia nauplii.The experiment was performed in 115-liter glass tanks containing 7000pieces of kutum larvae MoreThe aim of this study was investigation of growth rate, survival and activity of digestive enzymes (andalpha;-amylase and alkaline phosphatase) in fish larvae fed Artemia nauplii.The experiment was performed in 115-liter glass tanks containing 7000pieces of kutum larvae with an average initial weight of 5.1 mg (2 days after hatching).Samples for analysis of digestive enzyme activity were taken randomly at 2, 3, 5, 8, 10, 13, 17, 23, 27, 30.The results displayed that the stocking in high density to decrease growth efficiency but there was not considerable effect on survival rate larvae.In the present study was observed a significant increase in activity ofamylase from 23 to 30daysas the highest amylase activity was found at 30 days after hatching. The high content of glycogen and carbohydrates in live food may be to stimulate synthesis and secretion of amylase. An early peak of activity in alkaline phosphatase enzyme activity was observed at day 8 after hatching the indicating the maturation process in the intestines of kutum larvae. Manuscript profile -
Open Access Article
8 - Effect of Alkaline Phosphatase on the Enzymes and Proteins of Liver Tissue of Rat by Enzyme Inhibition
الهه سامانی جهرمی سمانه ذوالقدری جهرمیLiver is the largest gland in the body; which have different secretions. Alkaline phosphatase enzyme is one of them. The aim of this study is the investigation of alkaline phosphatase effect on the enzymes and proteins of liver in the male rats on the basis of enzyme in MoreLiver is the largest gland in the body; which have different secretions. Alkaline phosphatase enzyme is one of them. The aim of this study is the investigation of alkaline phosphatase effect on the enzymes and proteins of liver in the male rats on the basis of enzyme inhibition by L-Homo argenine. In this experimental study, 35 adult male rats were studied. They were randomly divided into 5 groups with seven rats in each group, including: control, sham and testing 1, 2 and 3 respectively. Control group were nourished by food and water; Sham group were injected just solvent. Group 1: 0.2 mg/ml alkaline phosphatase enzyme was injected. In Group 2: 2 mg/ml L-homo arginine inhibitors were injected. Group 3: First 2 mg/ml L-homo arginine was injected, then after 2 hours, 0.2 mg/ml alkaline phosphatase injection was done. All injections continued for 5 days. At the end of duration, a sample was prepared for evaluating ALP. Data was collected by using SPSS software and by one-way variance analytical tests (ANOVA) in significance P Manuscript profile -
Open Access Article
9 - The Effects of Hydroalcoholic Leaf Extract of Passiflora Caerulea on Activity of Liver Enzymes in Male Rats
طیبه Sadeghi مهرداد Shariati مختار MokhtariPassiflora caerulea is one of medicinal plants and use in medicinal industry. Passiflora caerulea contain high anti oxidant combinations this combinations pain properties and are useful in curing have anti cancer and anti inflammatory and antiepilepsy, diarrhea, burn, MorePassiflora caerulea is one of medicinal plants and use in medicinal industry. Passiflora caerulea contain high anti oxidant combinations this combinations pain properties and are useful in curing have anti cancer and anti inflammatory and antiepilepsy, diarrhea, burn, hemorrhoid and in regulating liver enzymes. In this experiential study, 40 wistar male rats, use weighting about 195-200 g each were used in five groups eight including :control without received nothing, sham group received distilled water and 3 experimental groups receiving 150,300,600, mg/kg leaves of passiflora caerulea Hydro-alcohol extract respectively. According to obtained results, ALT, AST and Albumin in experimental group receiving the highest dose of the extract 600 mg/kg, pation in control group, meaningful in level. The Level of ALP, Total Protein, Creatinin and total body weight to comparison by control group not show significant changes and hydro alcoholic extract Passiflora cearulea Author decrease level BUN to experimental groups extraction of receiving. Hydro-alcohol extract of Passiflora caerulea leaves probably has a significant effect on hepatic activity. This effect by anti oxidant property active, mechanisms via like free radical control and peroxides neutralization and prevention of revive glutathione, evacuate and cell membrane stabilization and by stimulating DNA polymerase and increasing rRNA synthesis and finally relief liver cell. Manuscript profile -
Open Access Article
10 - Determination of the diagnostic value of milk lactate dehydrogenase and alkaline phosphatase in bovine subclinical mastitis
عباس Kalantari SH Safi عباس Rahimi ForoshaniCurrently somatic sell count(SCC) and bacterial culture is considered as the gold standard of detecting subclinical mastitis.however,the ebove-mentioned tests have a low diagnostic accuracy.therefore,for identification of infected animals,new biomarkers with high clinic MoreCurrently somatic sell count(SCC) and bacterial culture is considered as the gold standard of detecting subclinical mastitis.however,the ebove-mentioned tests have a low diagnostic accuracy.therefore,for identification of infected animals,new biomarkers with high clinical accuracy are needed.the objective of this study was to determine the diagnostic value of milk lactate dehydrogenase(LDH) and alkaline phophatase(ALP_ for the diagnisis of subclinical mastitis in dairy cows.the activities of these enzymes increase during mastitis,which make them to be the potential biomarkers for screening of mastitis.a total of 145 subclinical healthy cows were randomly selected .of these 77 cows were considered to be affected by sub clinical mastitis based on a SCC higher than 100*1000 cells/ml of milk and positive bacterial culture results of milk samples obtained from at least one of the quarters.enzymes activities were measured in blood serum and defatted milk(centrifuged at 5000-g for 15 min at 4)using commercial kits.diagnostic sensivity and specificity and cutoff points for each test were determined via receiver-operating characteristics curve.significant (p<0.001)increases in the mean and median activities of LDH and ALP were found in the milk samples collected from cows with subclinical mastitis.milk LDH had the most clinical accuracy with 94.8% sensitivity and 94.1% specificity at cutoff point of 109 U/L.the results of the present study showed that the measurement of LDH and ALP activities in milk samples could be used as reliable method for detection of bovine subclinical mastitis. Manuscript profile -
Open Access Article
11 - The effect of eight weeks of high intensity interval and moderate intensity continuous trainings along with Citrus Aurantium on bone alkaline phosphatase and osteocalcin in the femur tissue of elderly female rats
Fatemeh Farkhaie سعید کشاورز Elham Eftekhari سید علی حسینیBackground and Purpose: Osteoporosis in old age is one of the most common metabolic bone diseases. Therefore, the aim of this study is to investigate the effect of eight weeks of high intensity interval training (HIIT) and moderate intensity continuous training (MICT) a MoreBackground and Purpose: Osteoporosis in old age is one of the most common metabolic bone diseases. Therefore, the aim of this study is to investigate the effect of eight weeks of high intensity interval training (HIIT) and moderate intensity continuous training (MICT) along with Citrus Aurantium (CA) on bone alkaline phosphatase (BALP) and osteocalcin (OCN) in the femur tissue of elderly female rats. Material and Methods: In this experimental study, 42 elderly female rats were randomly divided into 7 groups including 1) control (C), 2) MICT, 3) HIIT, 4) MICT+CA, 5) HIIT+ CA, 6) CA and 7) Sham (normal-saline). HIIT trainings were performed with an intensity of 85- 110% VO2max and MICT trainings were performed with an intensity of 65% VO2max; CA was consumed 300 mg/kg/day. Results: In the CA, MICT, HIITHIIT+CA and MICT+CA groups, BALP and OCN levels were higher than C group (P=0.001). In addition, BALP levels in MICT+CA group were higher than CA, HIIT, MICT and HIIT+CA groups. OCN levels in the MICT group were higher than the HIIT group (P=0.001). While in the HIIT and MICT group were higher than the HIIT+CA and MICT+CA groups (P=0.001). Conclusion: It seems that although HIIT, MICT and CA are effective (both alone and interactively) in improving bone metabolism, the effect of training is much stronger than the effects of CA on osteoporosis. Manuscript profile
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