Anti-inflammatory potential of aqueous extract and ethyl acetate fractions of Munronia pinnata (Wall) Theob. and the isolated compound, Senecrassidiol

Subject Areas : Natural Products: Isolation and Characterization

Swarna Hapuarachchi ¹ , Thusharie Suresh ² , Shiroma Hadunnetthi ³ , Wathuge Thamara Priyanthie Sandun Senarath ⁴ , Chandani Ranasinghe ⁵

1 - Unit of Pharmacology and Pharmaceutics, Department of Ayurveda, Institute of Indigenous Medicine, University of Colombo, Sri Lanka
2 - Department of Biochemistry, Faculty of Medical Sciences, University of Sri Jayewardenepura, Sri Lanka
3 - Institute of Biochemistry, Molecular Biology, and Biotechnology, University of Colombo, Sri Lanka
4 - Department of Botany, Faculty of Applied Sciences, University of Sri Jayewardenepura, Sri Lanka
5 - Department of Chemistry, Faculty of Natural Sciences, The Open University of Sri Lanka

Received: 2022-06-29 Accepted : 2022-09-18 Published : 2022-09-01

Keywords: <i>Munronia pinnata</i>, Wistar Rats, Anti-inflammatory, Membrane stabilization, Folk medicine, Senecrassidiol,

Abstract :

This study was designed to evaluate in vivo and in vitro anti-inflammatory potential of the different extracts of Munronia pinnata and compare it with the isolated compound, Senecrassidiol by using the carrageenan-induced paw edema animal model in Wistar rats. Munronia pinnata (Wall) Theob (Meliaceae) has been traditionally practiced for hundreds of years by traditional physicians as a home remedy for the treatment of inflammatory conditions in folk medicine in Sri Lanka. The tested ethyl acetate fractions of methanol extract and its isolated compound senecrassidiol exhibited significant anti-inflammatory activity in the tested models. It demonstrated the scientific rationale for the use of an anti-inflammatory agent in folk medicine. The MPaq showed a significant (p < 0.5) inhibition of infiltration of rat peritoneal cells, inhibition of NO production by rat peritoneal cells, and inhibition of membrane stabilization as well as the anti-histamine activity which are the probable anti-inflammatory mechanisms.

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