List of Articles نوشا ضیاء

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  1 - The evaluation and comparison of metabolic and oxidative stress status in dairy cows before and after parturition
  Maryam Maryam Karimi-Dehkord Forough Mohammadi Majid Gholami-Ahangarn
  Inroduction & Objective: In the recent years, due to the role of oxidative stress in the pathogenesis of various diseases, oxidative stress markers have become a useful tool in the prevention and treatment of various diseases. Regarding the importance of dairy cattl More

  Inroduction & Objective: In the recent years, due to the role of oxidative stress in the pathogenesis of various diseases, oxidative stress markers have become a useful tool in the prevention and treatment of various diseases. Regarding the importance of dairy cattle transition period and little research on the state of oxidative stress during the transition period, the aim of this study was to investigate the status of oxidative stress during the transition period using Malondialdehyde (MDA), glutathione (GSH) and total antioxidant capacity (GSH) two weeks before and after parturition in Holstein dairy cows in Shahrekord dairy farms. Materials and Methods: 42 dairy were selected from one of the industrial dairy farms in Shahrekord. Blood samples were taken two times (two weeks before and after pregnancy) and was transferred to the laboratory for measurement of Malondialdehyde, glutathione and total antioxidant capacity. T-test was used to compare the mean of each of these parameters before and after pregnancy. Results: The mean serum Malondialdehyde level after parturition in comparison to before parturition increased, although it was not statistically significant (P>0/05). The mean glutathione and total antioxidant capacity of the blood after delivery in comparison with the prepartum were significantly decreased (P<0/05). Also, the levels of superoxide dismutase, catalase and glutathione peroxidase enzymes decreased significantly after delivery compared to before delivery (P <0.05). Conclusion: According to the results of this study, it can be concluded that dairy cows are exposed to oxidative stress during the transition period, although they do not show clinical symptoms. As a result, it is important to pay attention to the use of antioxidants during the transition period. . Manuscript profile
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  2 - Frequency of qnrA and sul1 genes in Escherichia coli isolated from pericarditis and perihepatitis lesions of broilers in Isfahan province
  Mohammad Horri Majid Gholami-Ahangarn
  Inroduction & Objective: Antibiotic resistance can complicate the treatment of infectious diseases of livestock and poultry. The aim of this study was to determine the frequency of genes encoding resistance to quinolones and sulfonamides in Escherichia coli (E. coli More

  Inroduction & Objective: Antibiotic resistance can complicate the treatment of infectious diseases of livestock and poultry. The aim of this study was to determine the frequency of genes encoding resistance to quinolones and sulfonamides in Escherichia coli (E. coli) isolated from pericarditis and periphepatitis lesions in broilers to provide a suitable background for treatment with these drugs in these lesions.  Material and Methods: In this study, for detecting of resistance genes to fluoroquinolones and suvanamides, 50 bacterial strains were isolated from broiler chickens with pericarditis and periphepatitis and E. coli colonies were confirmed by microbial and biochemical tests. Then, the resistance of the strains to the commercial antibiotics (Enrofloxacin and sulfonamide + trimethoprim) was evaluated by the conventional antibiogram method. In addition, the bacterial genome was extracted by boiling method and the qnrA and sul1 genes were amplified with specific primers to evaluate antibiotic resistance against fluoroquinolones and sulfonamides. Results: In this study, 54% of enrofloxacin-resistant strains possed qnrA gene and 48% of sulfonamide-resistant strains plus trimethoprim contained sul1 gene. In this study, resistant strains without studied resistance genes were also found, which indicates the importance of other resistance genes in the development of resistance against sulfonamides and fluoroquinolones. Conclusion: Evaluation of antibiotic resistance against enrofloxacin and sulfonamides is not possible with the help of one gene and to accurately determine antibiotic resistance, routine phenotypic tests are more effective than detecting a specific gene. Manuscript profile
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  3 - Molecular detection of coronavirus causing infectious bronchitis in laying hens with cystic oviduct and quantitative and qualitative reduction of egg production
  Maryam Jalahi Majid Gholami-Ahangarn
  20.1001.1.17359880.1400.14.2.6.5
  Background and Aim: In this study, the contribution of IB coronavirus in egg quantitative and qualitative reduction syndrome was investigated.Methods: nine laying hen flocks attected to egg quantitative and qualitative reduction syndrom and 10 laying farms with a health More

  Background and Aim: In this study, the contribution of IB coronavirus in egg quantitative and qualitative reduction syndrome was investigated.Methods: nine laying hen flocks attected to egg quantitative and qualitative reduction syndrom and 10 laying farms with a healthy appearance were sampled. In addition, 6 farms with cystic oviduct were sampled in laying flocks, in Isfahan province. After extracting the genome, a fragment of 1200 bp of coronavirus S1 gene was amplified for identification of IB.Results: Out of 9 laying farms with quantitative and qualitative egg reduction syndrome and 10 apparently healthy farms, respectively 7 and 4 farms (78 and 40%) were infected with IB coronavirus based at least one positive sample. Out of 59 samples from farms with egg quantitative and qualitative reduction syndrome, 32 samples (54.2%) and out of 66 samples taken from apparently healthy farms, 7 samples (10.6%) were evaluated positive for IB coronavirus. In this study, all 6 farms with cystic oviduct had at least one positive sample for IB coronavirus and 31 samples out of 41 samples (73.17%) infected with IB coronavirus.Conclusion: IB coronavirus has a high share in the syndrome of quantitative and qualitative reduction of egg production in laying hens, but not all cases can be attributed to this virus and other infectious and non-infectious factors should be monitored. Due to the high frequency of this virus in cystic oviduct, it is necessary to identify the viral types that induce this disorder and to develop an appropriate control program. Manuscript profile
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  4 - The detection of virulence genes of shigatoxin and intimin from Escherichia coli isolated from a pigeon with gastrointestinal symptoms: a case report
  Majid Gholami-Ahangarn mehrdad Ostadpour Anita Khani
  Background & Aim : Escherichia coli (E. coli) is found as a flora in the digestive tract of all warm-blooded animals, including pigeons. This bacterium has many virulent genes including shigatoxin and intimin. Intimin is responsible for binding E. coli to intestinal More

  Background & Aim : Escherichia coli (E. coli) is found as a flora in the digestive tract of all warm-blooded animals, including pigeons. This bacterium has many virulent genes including shigatoxin and intimin. Intimin is responsible for binding E. coli to intestinal mucosal cells, and shigatoxin causes cell death. The aim of recent study was to investigate the presence of these virulence genes in E. coli isolated from a case of gastrointestinal infection in pigeons in order to determine the possibility of pathogenic E. coli with origin of pigeons in humans. Materials and Methods: In this regard, to identify virulent genes in E. coli isolated from diarrheic pigeon, a cloaca swab was prepared from a pigeon with diarrhea symptoms. After microbial culture and purification, the infection to E. coli was confirmed by biochemical examinations. Then, DNA was extracted by boiling method and hemolysin, intimin and sub unites of shigatoxin (stx1 & stx2) genes were detected. The target genes were identified by specific primers based on amplification of target genes with specific fragment lengths (165, 890, 614 and 779 bp, respectively). Results: The E. coli strain isolated from pigeon with symptom of diarrhea had the virulence genes of stx1 and intimin and lacks the genes encoding hemolysin and stx2. Conclusion: The results showed that the virulence genes of shigatoxin 1 and intimin were detectable in pigeons with gastrointestinal symptoms. According to these results, contrary to previous perceptions, birds may be the source of shigatoxogenic E. coli, and the symptoms of diarrhea in pigeons may be related to the presence of Shigatoxin and intimine in E. coli, which needs to be further investigated. Manuscript profile