The evaluation and comparison of metabolic and oxidative stress status in dairy cows before and after parturition

Subject Areas :

Maryam  Maryam Karimi-Dehkord ¹ (Department of clinical Sciences, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran)
Forough  Mohammadi ² (2Department of veterinary, Agriculture Faculty, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran)
Majid  Gholami-Ahangarn ³ (Department of Poultry Diseases, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran)

Received: 2021-04-12 Accepted : 2021-05-12 Published : 2022-02-20

Keywords: dairy cow, Oxidative stress, Transition Period, Metabolic index,

Abstract :

Inroduction & Objective: In the recent years, due to the role of oxidative stress in the pathogenesis of various diseases, oxidative stress markers have become a useful tool in the prevention and treatment of various diseases. Regarding the importance of dairy cattle transition period and little research on the state of oxidative stress during the transition period, the aim of this study was to investigate the status of oxidative stress during the transition period using Malondialdehyde (MDA), glutathione (GSH) and total antioxidant capacity (GSH) two weeks before and after parturition in Holstein dairy cows in Shahrekord dairy farms. Materials and Methods: 42 dairy were selected from one of the industrial dairy farms in Shahrekord. Blood samples were taken two times (two weeks before and after pregnancy) and was transferred to the laboratory for measurement of Malondialdehyde, glutathione and total antioxidant capacity. T-test was used to compare the mean of each of these parameters before and after pregnancy. Results: The mean serum Malondialdehyde level after parturition in comparison to before parturition increased, although it was not statistically significant (P>0/05). The mean glutathione and total antioxidant capacity of the blood after delivery in comparison with the prepartum were significantly decreased (P<0/05). Also, the levels of superoxide dismutase, catalase and glutathione peroxidase enzymes decreased significantly after delivery compared to before delivery (P <0.05). Conclusion: According to the results of this study, it can be concluded that dairy cows are exposed to oxidative stress during the transition period, although they do not show clinical symptoms. As a result, it is important to pay attention to the use of antioxidants during the transition period. .

References:

1.Adela, P., Zinveliu, D., Pop, R.A., Andrei, S., Kiss, R. (2006). Antioxidant status in dairy cows during lactation. Buletin USAMV-CN, 50(12); 1454-2382.

2.Balari Mahyari, F., Karimi Dehkordi, M., Nazem, M.R. (2020). Evaluation of oxidative status in dairy cattle with subclinical ketosis and clinical endometritis. Veterinary Clinical Pathology, 13(52); 385-397 [In Persian]

3.Bell, A.W. (1995). Regulation of organic nutrient metabolism during transition from late pregnancy to early lactation, J Anim Sci, 73; 2804-2819

4.Benzie, I.F., Szeto, Y.T. (1999). Total antioxidant capacity of teas by the ferric reducing/antioxidant power assay. J Agri Food Chem, 47(2); 633-636.

5.Bernabucci, U., Ronchi, B., Lacetera, N., Nardone, A. (2002). Markers of oxidative status in plasma and erythrocytes of transition dairy cows during hot season. J Dairy Sci, 85; 2173-2179.

6.Bernabucci, U., Ronchi, B., Lacetera, N., Nardone, A. (2005). Influence of body condition score on relationships between metabolic status and oxidative stress in periparturient dairy cows. J Dairy Sci, 88(6); 2017-2026.

7.Bilici, M., Efe, H., Köroğlu, M.A., Uydu, H.A., Bekaroğlu, M., Değer, O. (2001). Antioxidative enzyme activities and lipid peroxidation in major depression; alterations by Ellman G.L. 1959. Tissue sulfhydryl groups. Arch Biochem Bioph, 82; 70–77.

8.Birben, E., Sahiner, U.M., Sackesen, C., Erzurum, S., Kalayci, O. (2012). Oxidative stress and antioxidant defense. World Allergy Organ J, 5(1); 9-19.

9.Buelo, A., Hernandez, J., Benedito, J.L., Castillo, C. (2016). Association of oxidative status and insulin sensitivity in periparturient dairy cattle; An observational study. J Anim Physiol An N, 100; 279-286.

10.Castillo, C., Hernandez, J., Bravo, A., Lopez-Alonso, M., Pereira, V., Benedito, J.L. (2005). Oxidative status during late pregnancy and early lactation in dairy cows. Vet J, 169(2); 286-92.

11.De Quiroga, G.B., Lopez-Torres, M., Perez-Campo, R. (1992). Relationship between antioxidants, lipid peroxidation and aging.  Free radicals and aging: Springer; 109-123.

12.Divers, T.J., Peek, S.F. (2008). Rebhum's diseases of dairy cattle. (2th ed.) Saunders Elsevier publishing. Philadelphia, USA.

13.Drackley, J.K. (1999). Biology of dairy cows during the transition period: The final frontier? J Dairy Sci, 82; 2259-2273.

14.Duffield, T. (2000). Subclinical ketosis in lactating dairy cattle. Vet Clin North Am Food Anim Pract, 16; 231-253.

15.Gaál, T., Ribiczeyné-Szabó, P., Stadler, K., Jakus, J., Reiczigel, J., Kövér, P. (2006). Free radicals, lipid peroxidation and the antioxidant system in the blood of cows and newborn calves around calving. Comp Biochem Physiol B,  Biochem Mol Biol, 143(4); 391-396.

16.Grummer, R.R. (1995). Impact of changes in organic nutrient metabolism on feeding the transition dairy cow. J Anim Sci, 73; 2820-2833.

17.Hayes, J.D., McLellan, L.I. (1999). Glutathione and glutathione-dependent enzymes represent a Co-ordinately regulated defence against oxidative stress. Free Radic Res, 31(4); 273-300.

18.Herdt, T.H. (2000). Ruminant adaptation to negative energy balance. Vet Clin N Am-Food A, 16(2); 215-230.

19.Konvičná, J., Vargová, M,. Paulíková, I., Kováč, G., Kostecká, Z. (2015). Oxidative stress and antioxidant status in dairy cows during prepartal and postpartal periods. Acta Vet Brno, 84(2); 133-140.

20.Maurya, P.K., Aggarwal, A., Singh, S., Chandra, G., Singh, A., Chaudhari, B. (2014). Effect of vitamin e and zinc on cellular antioxidant enzymes in karan fries cows during transition period. Indian J Anim Res, 48(2); 109-119.

21.Mittler, R. (2002). Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci, 7 (9); 405-410.

22.Mousavi Tashar, N., Karimi Dehkordi, M., Nazem, M.R. (2020). The effect of serum catalase and superoxide dismutase activity on subclinical ketosis and conception rate at first service in Holstein dairy cows. Iranian veterinary Journal, accepted [In Persian]

23.Mulligan, F.J., Doherty, M.L. (2008). Production diseases of the transition cow. Vet J, 176(1); 3-9.

24.Mytilineou, C., Kramer, B.C., Yabut, J.A. (2002). Glutathione depletion and oxidative stress. Parkinsonism Relat Disord, 8; 385–387.

25.Nielsen, F., Mikkelsen, B.B., Nielsen, J.B., Andersen, H.R., Grandjean, P. (1997). Plasma malondialdehyde as biomarker for oxidative stress: reference interval and effects of life-style factors. Clin Chem, 43(7);1209-1214.

26.Pintea, A., Zinveliu, D., Pop, R.A., Andrei, S., Kiss, E. (2006). Antioxidant status in dairy cows during lactation. Bull Univ Agric Sci Vet Med Cluj Napoca Vet Med, 63(1-2);110-120.

27.Píšťková, K., Kazatelová, Z., Procházková, H., Danielová, L., Illek, J. (2018). Antioxidant status and concentration levels of malondialdehyde(MDA) in dairy cows during periparturient period. Magy Allatorvosok. Proceedings, XVIII Middle-European Biuatrics Congress; 120-123.

28.Píšťková, K., Illek, J., Kadek, R. (2019). Determination of antioxidant indices in dairy cows during the periparturient period. Acta Vet Brno, 88(1); 3-9.

29.Safari, M., Ghasemi, E., Alikhani, M., Ansari-Mahyari, S. (2018). Supplementation effects of pomegranate by-products on oxidative status, metabolic profile, and performance in transition dairy cows. J Dairy Sci, 101(12); 11297-11309.

30.Sharma, N., Singh, N., Singh, O., Pandey, V., Verma, P. (2011). Oxidative stress and antioxidant status during transition period in dairy cows. Asian-Austral J Anim Sci, 24(4); 479-84.

31.Sordillo, L.M., Aitken, S.L. (2009). Impact of oxidative stress on the health and immune function of dairy cattle.Vet Immunol Immunopathol, 15;104-109.

32.Timothy, A., Reinhardt, T., John, D., Lippolis. B., Brian, J., McCluskey, J.P., Goff Ronald, L. (2011). Prevalence of subclinical hypocalcemia in dairy herds. Vet J, 188; 122-124.

33.Toescu, V., Nuttall, S.L., Martin, U., Nightingale, P., Kendall, M.J., Brydon, P. (2002). Changes in plasma lipids and markers of oxidative stress in normal pregnancy and pregnancies complicated by Diabetes. Clin Sci, 106(1); 93-98.

34.Wachter, C.M., McDaniel, B.T., Whitlow, L.W., Pettyjohn, S. (1999). Genetics of antioxidant activity in Holsteins and Jerseys: associations with various traits. J Dairy Sci, 82(Suppl. 1); 31-38.

35.Walsh, R.B., Walton, J.S., Kelton, D.F., LeBlanc, S.J., Leslie, K.E., Duffield, T.F. (2007). The effect of subclinical ketosis in early lactation on reproductive performance of postpartum dairy cows. J Dairy Sci, 90; 2788-2796.

36.Wathes, D.C., Bourne, N., Cheng, Z., Mann, G.E., Taylor, V.J. Coffey, M.P. (2007). Multiple correlation analyses of metabolic and endocrine profiles with fertility in primiparous and multiparous cows. J Dairy Sci, 90; 1310-1325.

37.Weiss, W.P., Hogan, J.S. (2005). Effect of selenium source on selenium status, neutrophil function, and response to intramammary endotoxin challenge of dairy cows. J Dairy Sci, 88(12); 4366-4374.