The effect of eight weeks of HIIT and N-chromosome royal jelly on liver enzymes and insulin resistance index in type 2 diabetic obese rats

Subject Areas :

sahar Riasati ¹ , Hossein Abednatanzi ² , Mandana Gholami ³

1 - PhD student, Department of Physical Education and Sports Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2 - Department of Physical Education and Sports Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.
3 - Department of Physical Education and Sports Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Received: 2022-06-17 Accepted : 2022-07-30 Published : 2023-01-21

Keywords: HIIT, Royal Jelly, Liver Enzyme, type 2 diabetes, mice,

Abstract :

Background & Aim: Type 2 diabetes is the most common endocrine disease that occurs due to glucose intolerance due to imbalance between reserves and insulin demand, which leads to be changes in liver enzymes with structural and functional changes in the liver. The aim of this study was to study changes in liver enzymes and insulin resistance index after HIIT and royal jelly in obese rats with type 2 diabetes. Materials & Methods: The statistical sample of the present study was 36 obese male rats with an average weight of 409 gr. After 20 weeks of high-fat diet, rats became diabetic by intraperitoneal injection of 25 mg/kg STZ body weight. Mice with fasting glucose between 150 and 400 mg /dl were considered to have type 2 diabetes. Diabetic rat in 4 groups of control (N=6), HIIT (N=8), royal gel (N=7), HIIT& Royal gel (N=8) group done and Accomplished Eight weeks of HIIT, 5d/w with intense 2-minute intervals of 80 to 90% VO2max and one-minute rest intervals with 50 to 56% VO2max. Royal jelly was given by gavage at the rate of 100 mg / kg 5 days a week. At the end of the protocol, blood samples were taken and liver enzymes and glucose were measured using an auto-analyzer and insulin was measured using a laboratory kit and analyzed using one-way and two-way analysis of variance and post hoc test. Results: showed that in comparison with the control group, HIIT led to a significant decrease in glucose and insulin resistance index and intermittent exercise resulted in a decrease in liver enzymes ALT, AST and ALP compared to the control group. The ALP decrease was significant (P = 0.001). Conclusion: It seems that HIIT and consumption of royal jelly can help reduce liver enzymes and improve liver status in rats.

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