Correlation between wnt-1 and Bcl2 genes expression in adenocarcinoma gastric cancer cells treated with turmeric extract
Subject Areas : Plasma biomarkers
Zahra Deilami Khiabani
1
,
Mohtada Gholami Gheibi
2
1 - Biology Department, Faculty of Basic Science and Engineering, Islamic Azad University, Zanjan Branch, Zanjan Iran.
2 - Islamic Azad University, Zanjan Branch, Zanjan ,Iran
Keywords: Gastric cancer, Wnt1, Bcl-2, Turmeric extract, AGS,
Abstract :
Background & Aim: Adenocarcinoma is a prevalent form of gastric cancer. Wnt-1, contribute to the tumorigenic processes when aberrantly activated. Concurrently, Bcl-2, an anti-apoptotic protein, is linked to the survival of cancer cells, facilitating resistance to apoptosis and complicating treatment efforts. Recent studies suggest that turmeric extract, may offer a therapeutic mechanism in cancer, particularly in the context of gastric cancer. The aim of this study is to evaluate wnt1and Bcl2 genes expression in AGS cell lines treated with turmeric extract.
Materials & Methods: AGS cells have cultured in DMEM with FBS 10% Penstrep 1% and Co2 % 5 and humidity of 85%. Then cells were treated with concentrations of 800, 1200 and 2000μg / ml of the turmeric extract for 48 and 72 hours. Extraction of RNA and cDNA synthesis was performed using the kits. The expression of genes was investigated using real-time PCR.
Results: Results have shown that both genes expression are decreased in all treatments. The most significant decrease for both genes was in 72 hours treatment with turmeric extract. The expression of Bcl-2 gene with 76% decrease has been shown in 2000 μg/ml and for wnt-1 gene with 52% decrease in 1200 μg/ml concentration.
Conclusion: Turmeric has been shown to reduce expression of anti-apoptotic proteins like Bcl-2 and wnt-1, thereby promoting apoptosis in cancer cells and avoid metastasis.
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