Detection of blaCTX-M, blaTEM and blaSHV genes in Enterobacter cloacae isolates
Subject Areas : Journal of Quality and Durability of Agricultural Products and Food Stuffs
1 - گروه زیست شناسی دانشگاه آزاد اسلامی واحد شهرکرد
Keywords: Enterobacter cloacae, ESBLs, Antibiotic resistance,
Abstract :
Enterobacter cloacae strains, with having different virulence factors and multiple antibiotic resistances, are mainly considered as an opportunistic pathogen in nosocomial infections. The aim of this study was to determine the antibiotic resistance pattern of Enterobacter aerogenes isolates isolated from urinary tract infections in Shahrekord city and to detect broad-spectrum beta-lactamase (SHV-CTX, TEM) genes in these strains. In this study, 65 Enterobacter cloacae strains were studied. The antibiotic resistance of the isolates and the frequency of broad-spectrum beta-lactamase genes were studied phenotypically according to CLSI by instructions using a combined disk and genotypic method in the presence of specific primers. Out of 65 isolates of Enterobacter cloacae, 25 isolates (38.26%) were found to produce ESBLs in the phenotypic study, and the frequency of blaCTX-M, blaTEM and blaSHV genes was reported as 28%, 32% and 10%, respectively. In the statistical analysis, no significant statistical relationship was observed between resistance to the antibiotics and bla genes. The present study indicates high prevalence ESBLs producing Enterobacter cloacae strains. The present study suggests that ESBL producing Enterobacter cloacae isolates have a high prevalence. The increase in the number of these species is often due to the irrational administration of antibiotics, which requires the use of new antimicrobial agents and limiting the unnecessary use of antimicrobial agents and increasing the use of infection control tools.