On a 7-years-old dog with Azoospermia three conventional drugs in breeding techniques were tested to assess the
effects and reproductive improvement of them. the Drugs that chosen include gonadotropin (GnRH) in commercial
name Cystorelin، 50 micrograms subcutaneously 60 minutes prior to sperm collection , prostaglandin (PGF2α)
in commercial name Veteglan , 0/005 mg/kg subcutaneously 15 minutes prior to sperm collection and Oxytocin
10IU per dog intramuscularly 10 minutes prior to sperm collection. Semen collected from this animal by manual
semen collection technique and we had 7 days sexual rest between each collection. during the semen collection the
dog’s libido was assessed and then semen Volume , Semen PH , semen color , semen concentration per ml ,total
count of spermatozoa , progressive motility forward percent , morphologically normal spermatozoa percent and
Total normal motile spermatozoa (TMNS) was evaluated. The samples did not show any traces of semen quality
change when dog received prostaglandin and oxytocin and there is no spermatozoa in that samples. But when the
sampled animal was under the influence of gonadotropin therapy semen quality improved dramatically in addition
we have one billion and twenty-four millions of spermatozoa as the total count of the sample. Two hypotheses are
proposed for this quality improvement. The frst one say the prescribing of gonadotropin in this dog, increased the
animal blood testosterone index and stimulate the seminiferous ducts to released spermatozoa. The second one
say because of the clinical condition of the animal it seems to had IHH syndrome and during the administration of
gonadotropin the body hormonal system was regulated and released the sperm cells. Finally, it can be concluding
that the use of gonadotropins may be improving the quality of semen of animals that have Azoospermia with
source of hormonal abnormality.
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