Studying the Concentration of Glucose, Urea and Cholesterol in Blood Serum and Follicular Fluid of Different-Sized Follicles in Hybrid Cows of Khouzestan Province

Subject Areas : clinical veterinary science

کمال  Hasanpoor, ¹ (Department of Animal Sciences, University of Agricultural Sciences and Natural Resources Ramin Khuzestan. Khozestan. Iran)
محمد تقی  Tagi Begi Nasiri, ² (Department of Animal Sciences, University of Agricultural Sciences and Natural Resources Ramin Khuzestan. Khozestan. Iran)
منصور  Khezri, ³ (Department of Animal Sciences, University of Zanjan. Iran)
, M.  Asghari Moghadam ⁴ (Department of Animal Sciences, University of Zanjan. Iran)
A.  Karamzade, ⁵ (Department of Animal Sciences, University of Agricultural Sciences and Natural Resources Karaj, Tehran. Iran.)
M.  Amrai, ⁶ (Department of Animal Sciences, University of Agricultural Sciences and Natural Resources Tarbeyat Modares. Iran.)

Received: 2016-11-03 Accepted : 2016-11-03 Published : 2013-12-22

Keywords: Cholesterol, glucose, Urea, Follicular Fluid Ovarian and Blood Serum,

Abstract :

Improvement of the fertility and reproductive status of cows necessitates having suffcient knowledge of the ovary of this animal species. Ovarian follicular activities play a signifcant role in controlling reproductive cycle, ovulation and pregnancy. The present study aims to compare the concentration of glucose, urea and cholesterol of ovarian follicular fluid in different-sized follicles with the blood serum of hybrid cows. Blood samples and ovaries of 20 slaughtered cows were examined. The follicular fluid was obtained from 3 follicular groups of small (3-5mm), average (6-9mm) and large (10mm or more) sizes. Concentration of glucose, urea and cholesterol in follicular fluids and blood serum was determined. The results showed that concentration of glucose, urea and cholesterol in blood serum was signifcantly higher than that of the large, average-sized and small follicles. Glucose concentration of large follicles was signifcantly higher than that of the average-sized and small follicles (P < 0.05). However, no signifcant difference was observed between the average-sized and small follicles. Urea level decreased as the follicle size increased. Cholesterol concentration of small follicles was signifcantly higher than that of the large and average-sized follicles (P < 0.05). However, no signifcant difference was observed between the large and average-sized follicles. Overall, the results showed that ovarian follicle size grows and matures in a changing biochemical environment. This environment is associated with the changes in blood glucose level. Moreover, urea and cholesterol levels decrease as the follicle size increases.