The Teratogenic Effects Pesticide of Diazinon On the development of Balb/C Mouse Embryos 3Th to 6Th Days Of Pregnancy

Subject Areas : clinical veterinary science

safoora saffari ¹ , Parvin Torabzadeh ² , Saba Rastgar Gharahshiran ³

1 - Islamic Azad University, karaj
2 - Phd, Assistant Professor, Department of Developmental Biology, Karaj Branch, Islamic Azad University, karaj,
3 - M.Sc,Department of Developmental Biology, Karaj Branch, Islamic Azad University, karaj, Iran

Received: 2017-12-12 Accepted : 2018-08-24 Published : 2019-02-20

Keywords: diazinon, Teratogen, Mouse embryo, Agricultural pesticides,

Abstract :

Introduction:Diazinon is one of agricultural pesticides and organophosphate pesticide, Because the chemical structure and the effects have been devastating on nervous, respiratory and digestive systems in recent years there have been studied. But It is effects on the embryo especially during pregnancy has not been reviewed.Therefore in study the teratogenic effects pesticide of Diazinon on the development of Balb/C mouse embryos 3Th to 6Th days of pregnancy.Material and Method:In this study 50 female Balb/C mice were randomly divided into 6 equal groups acontrol group(non-injection:5mice) and witess(injection of saline:5mice) and 4 experimental groups(each group: 10 mice).A lethaldose LD50 was determined in condition of 11.09 ml/kg.bw in vivo and selected dose for injection 0.4 ml/kg.bw.Injection was done on the 3Th to 6Th days of pregnancy by enema Then the mouse were sacrificed on day 15 of pregnancy.For security of results above experiences was repeated three times.Data was checked with SPSS17 software and Duncan test subjest to (P