List of Articles SH Safi

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  1 - Determination of oxidative stress indicator in dairy cattle with subclinical mastitis
  ام البنین  GHasemian karik SH Safi
  20.1001.1.20088159.1391.3.1.2.5
    The most important and common disease among dairy cows is mastitis and it is still a big challenge for the dairy industry all over the world.Oxidative stress induced by reactive oxygen species(ROS) is a major cause of different diseases especially mastitis in ca More

    The most important and common disease among dairy cows is mastitis and it is still a big challenge for the dairy industry all over the world.Oxidative stress induced by reactive oxygen species(ROS) is a major cause of different diseases especially mastitis in cattle.there are few studies on the role of ROS in occurrence of subclinical mastitis.the objective of the persent study was to evaluate the changes in plasma malondialdehyde concentrations as a marker of lipid peroxidation of red blood cells and its relationship with somatic cell count(SCC) in milk as the marker of mammary inflammation in cattle.Heparinized blood and milk samples were collected from 45 healthy cows and 45 cows with subclinical mastitis from dairy farms in Tehran province,Iran.No significant difference (p>0.05) was seen between concentrations of malondialdehyde in the studied groups.there was a significant difference (p<0.001) between SCCs in the healthy cows with subclinical mastitis.the concentration of plasma malondialdehyde was higher in cows with subclinical mastitis which indicates mild increase in production of ROS in response to invading pathogens.     Manuscript profile
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  2 - Determination of the diagnostic value of serum amyloid A, haptoglobin and some hematologic parameters in assessment of horse health
  S.M Mortazavi SH Safi S.H Shirazi Beheshtiha وحید Rabani
  20.1001.1.20088159.1391.3.2.1.6
    Acute phase proteins (APPs) are a class of serum proteins whose their concentration increase remarkably in response to infection,inflammation,trauma,neoplasia and etc.haptoglobin(HPt) and serum Amyloid (SAA) are to major APPs in horse.since,in farms, differentia More

    Acute phase proteins (APPs) are a class of serum proteins whose their concentration increase remarkably in response to infection,inflammation,trauma,neoplasia and etc.haptoglobin(HPt) and serum Amyloid (SAA) are to major APPs in horse.since,in farms, differentiation between healthy and sick horse is so important,the persent study was designed to determine the diagnostic value of APPs as sensitive biomarkers,and compare it with hematologic parameters for differentiation of healthy and sick horses in farm.according to the results of the present study, increase in the median of HPt and SAA was significant.with regard to the ROC curve,cutoff point of HPt and SAA was 0.829 and 0.6 ng/dl respectively and sensitivity and specificity in their cut off point was 86%, 87% for HPt and 84% , 73% for SAA. in addition, clinical accuracy of HPt and SAA,base of area under the curve,was 0907, 0.891 respectively. in other hand, significant difference between the clinical accuracy of HPt and SAA has not be seen.results of the present study show that HPt and SAA measurement can be used as a screening test to determine health status in horse. Manuscript profile
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  3 - The prevalence of subclinical ketosis in dairy farms and its relation to periparturient disorders in city of shahrekord
  مهدی Sakha SH Nejat SH Safi
  20.1001.1.20088159.1391.3.4.2.1
  This study was carried out to determine the prevalence of subclinical ketosis and relation to periparturient disorders in dairy farm in shahr-e-kord.a total 203 multiparous holstein cows(parity 2-9) were randomly selected from 10 commercial dairy herds.since the likely More

  This study was carried out to determine the prevalence of subclinical ketosis and relation to periparturient disorders in dairy farm in shahr-e-kord.a total 203 multiparous holstein cows(parity 2-9) were randomly selected from 10 commercial dairy herds.since the likely risk time for occurrence of ketosis is during 2-5 weeks after parturition, the blood samples were taken wk 1 before calving and at wk 2 and 3 after calving.from 3 weeks before calving to 3 months after calving,peripartum diseases informations including milk fever,metritis,mastitis,clinical ketosis,displased abomasum,retained placenta,dystocia,pneumonia and lamemess were gathered in data sheet form.beta-hydroxy butyrate and glucose were measured in serum by using automated analyzer and statistical analysis was performed to investigate the prevalence of ketosis(clinical and subclinical) and related periparturient diseases.in this study,the prevalence of subclinical ketosis,using cut-off 1200mmol/lit of beta-hydroxy butyrate was 0.98 in wk 1 before,9.8 in wk 2 and 13.79 percent in wk 3 after parturition respectively.it was shown that there is a significant correlation between subclinical ketosis and left displacement of abomasum,metritis and mastitis(p<0.05), scparately so that the odds ratio of each disease was 9.74, 4.26 and 6.64 respectively.       Manuscript profile
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  4 - Determination of the diagnostic value of milk lactate dehydrogenase and alkaline phosphatase in bovine subclinical mastitis
  عباس Kalantari SH Safi عباس Rahimi Foroshani
  20.1001.1.20088159.1391.3.4.4.3
  Currently somatic sell count(SCC) and bacterial culture is considered as the gold standard of detecting subclinical mastitis.however,the ebove-mentioned tests have a low diagnostic accuracy.therefore,for identification of infected animals,new biomarkers with high clinic More

  Currently somatic sell count(SCC) and bacterial culture is considered as the gold standard of detecting subclinical mastitis.however,the ebove-mentioned tests have a low diagnostic accuracy.therefore,for identification of infected animals,new biomarkers with high clinical accuracy are needed.the objective of this study was to determine the diagnostic value of milk lactate dehydrogenase(LDH) and alkaline phophatase(ALP_ for the diagnisis of subclinical mastitis in dairy cows.the activities of these enzymes increase during mastitis,which make them to be the potential biomarkers for screening of mastitis.a total of 145 subclinical healthy cows were randomly selected .of these 77 cows were considered to be affected by sub clinical mastitis based on a SCC higher than 100*1000 cells/ml of milk and positive bacterial culture results of milk samples obtained from at least one of the quarters.enzymes activities were measured in blood serum and defatted milk(centrifuged at 5000-g for 15 min at 4)using commercial kits.diagnostic sensivity and specificity and cutoff points for each test were determined via receiver-operating characteristics curve.significant (p<0.001)increases in the mean and median activities of LDH and ALP were found in the milk samples collected from cows with subclinical mastitis.milk LDH had the most clinical accuracy with 94.8% sensitivity and 94.1% specificity at cutoff point of 109 U/L.the results of the present study showed that the measurement of LDH and ALP activities in milk samples could be used as reliable method for detection of bovine subclinical mastitis.     Manuscript profile