Background & Objectives: Pasteurella multocida is an opportunistic microorganism responsible for haemorrhagic septicaemia in cattle and buffalo and pneumonia in sheep and goats. Adhesion factors play crucial role in attachment to cell surfaces, host invasion and col
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Background & Objectives: Pasteurella multocida is an opportunistic microorganism responsible for haemorrhagic septicaemia in cattle and buffalo and pneumonia in sheep and goats. Adhesion factors play crucial role in attachment to cell surfaces, host invasion and colonization. The aim of this study was to identify the frequency of adhesion genes from P. multocida isolated livestock. Materials & Methods: This study was performed on 50 samples isolated from ailing sheep, goats and cattle. Amplification of kmt1 gene with the aim of determining the molecular identity of the isolates and identifying adhesion genes (fimA, hsf1 and hsf2) were performed by using simple PCR method.
Results: PCR results show that, the frequency of fimA and hsf2 genes were 44% and 80%, respectively. In addition, hsf1 did not exist in the isolates. hsf2 was the only adhesion gene which was identified in cattle. So it may be plays a crucial role in invasion of this host. Moreover, it was found that there is a significant correlation between hsf2 gene and capsule type D.
Conclusion: In the present study, new information was obtained regarding the frequency of adhesion genes in P.multocida strains isolated from multiple livestock in Shiraz. Investigating the pathogenicity role of adhesion genes in laboratory animals and determine the role of these factors in immunity to pasteurellosis are recommended.
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