Background & Objectives: Xylitol is a naturally five–carbon polyol with a high sweetening ability. The biotechnological production of xylitol by microorganisms specially yeasts is very environmentally safe and has been studied as an alternative to the chemical More
Background & Objectives: Xylitol is a naturally five–carbon polyol with a high sweetening ability. The biotechnological production of xylitol by microorganisms specially yeasts is very environmentally safe and has been studied as an alternative to the chemical method. The purpose of this study was xylitol production by Candida guilliermondii that isolated from spores of Zinnia.
Materials & Methods: The xylitol producing yeast strain was isolated on Yeast extract Pepton Xylose (YePX) medium. The identity of strains was confirmed using PCR reaction. The xylitol produced by the strains was analyzed using tine layer chromatography (TLC) and specific kits. The concentration of Xylitol produced in this experiment was quantified using HPLC method.
Results: The xylitol producing yeast strain was isolated from pollen of Zinnia elegans flower and was identified by 16S rRNA sequence analysis as Candida guilliermondii. This strain produced 24.67 g l-1 xylitol after 72 hours in medium contained 40 g l-1 xylose.
Conclusion: In this study xylitol was produced by Candida guilliermondii isolated from the nature. According to the properties of xylitol and the difficulties through chemical production of this compound, the sweetener produced by biotechnological methods can be used as an attractive sugar in the nutritional supplement and pharmaceutical industries.
Manuscript profile
Background & Objectives: Sorbitol is a 6- carbon polyol, which is used in food industries as a precursor for production of many products due to its sweetness and high solubility. The application of microbial process for sorbitol production has recently been becoming More
Background & Objectives: Sorbitol is a 6- carbon polyol, which is used in food industries as a precursor for production of many products due to its sweetness and high solubility. The application of microbial process for sorbitol production has recently been becoming of point of interest because of requirement for suitable fermentative conditions, being cost effective and less environmental pollutions. The purpose of this study was to produce sorbitol from sucrose by Debaryomyces hansenii isolated from the nature.
Materials & Methods: The sorbitol producing yeast strain was isolated in a medium containing 40% glucose and 1% yeast extract. Following identification of yeast using ITS gene sequencing, we focused on the process of fermentation and production of sorbitol from sucrose. The quality and quantity of sorbitol production was analyzed by thin layer chromatography, colorimetric method and Megazime kit.
Results: The sorbitol producing yeast strain was isolated from pollen of Malva sylvestris flower, and was identified as D. hansenii. This strain produced 5.23 g l-1 sorbitol after 5 days in medium containing of 150 g l-1 sucrose.
Conclusion: In this study sorbitol was produced by a D. hansenii isolated from the nature. Due to high efficiency of sorbitol production from sucrose by yeast, isolation of sorbitol producing native yeasts and optimization of the production conditions can be helpful for increases in quality and quantity of sorbitol production.
Manuscript profile