Background & Objectives: Bioremediation is a technology to remove oil pollution. Microbial bioremediation id the best technology to remove contaminants in which pollutants are converted to non-toxic chemicals in expenses of lowest amount of energy, chemicals and tim
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Background & Objectives: Bioremediation is a technology to remove oil pollution. Microbial bioremediation id the best technology to remove contaminants in which pollutants are converted to non-toxic chemicals in expenses of lowest amount of energy, chemicals and time. This study aimed to isolate and identify the petroleum hydrocarbons degrading bacteria from oil reservoirs located at Asmari Ahwaz. Materials & Methods: This field study was performed in the oil reservoirs located at Asmar, Ahvaz. The primary isolation was performed using a salt containing base medium through a multistep process, and the salt tolerance of isolates were tested by this medium. The elimination of the hydrocarbons by isolated bacteria were studied using gas chromatography. The identity of bacteria was determined based on biochemical tests and sequencing of 16S rRNA gene. Results: In this study, a halotolerant Gram-positive bacteria, belonged to Streptomyces, was isolated from the field. This isolate showed an acceptable growth into 7.5% salt concentration and was able to use oil as the sole source of carbon. Also, this strain was able to reduce the level of hydrocarbons to 71.58 % through incubation in the saline medium for 10 days. Conclusion: According to the results, the isolated strain is capable to tolerate high concentrations of salts and is desirable to remove the hydrocarbons, which is beneficiary due to the difference in salt concentration in contaminated areas. As a result, this isolate can be useful for removal of pollutants from the environment and reduction of their side effects on life.
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