Background and Objectives: Finegoldia magna is a potential opportunistic pathogen for humans. F. magna as the most frequent pathogenic species of gram positive anaerobic cocci accounts for up to 5-12% of all anaerobic infections. F. magna possess Protein L super antigen More
Background and Objectives: Finegoldia magna is a potential opportunistic pathogen for humans. F. magna as the most frequent pathogenic species of gram positive anaerobic cocci accounts for up to 5-12% of all anaerobic infections. F. magna possess Protein L super antigen and Type IV Pilin PilA as invaluable proteins for designing multi epitope peptide vaccines in current study.Materials and Methods: In this study, immunoinformatics tools were used to predict B and T cell epitopes of Protein L and Type IV Pilin PilA. The epitopes were evaluated for antigenicity, allergenicity and binding energy to appropriate HLA alleles and then were fused together by GPGPG and EAAAK spacers. Vibrio cholera Toxin B Subunit was introduced at N-terminus of the constructed vaccine as adjuvant, and with an eye on further identification and purification, a 6×HisTag was introduced at C-terminus. Codon optimization performed for further expression in Escherichia coli host. The amino acid sequence of the multi epitope peptide vaccine used for 3D structure prediction and refinement. Then structural evaluation via ramachandran plot analysis performed. Physicochemical properties and solubility of the constructed vaccine was also studied.Results: Results showed the selected epitopes with high antigenicity and no allergenicity. These epitopes manifest high affinity toward recommended HLA alleles. The predicted 3D model of constructed vaccine showed high stability, solubility and half-life for expression in E. coli host. Conclusion: In this study, Protein L and Type IV Pilin PilA used for in-silico designing an effective vaccine against F. magna. Further in-vitro/vivo studies are recommended.
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