Background & Objectives: Pseudomonas aeruginosa is a common opportunist pathogen in hospitals and the etiologic agent of the majority of infections in human beings. This study aimed to genotyping of P. aeruginosa isolated from hospital infections. Materials & Methods: This cross-sectional study was performed on 18 isolates of P. aeruginosa isolated from hospitalized patients in Shahrekord hospitals. We applied three different methods, RAPD-PCR, Rep-PCR and ERIC-PCR for genotyping of the isolates. Results: Based on RAPD-PCR method, overall 86 different bands of DNA with a range of 300 to 1000 bps were obtained from the under study isolates and among them, 74 bands were polymorphic. Analysis of P. aeruginosa isolates based on ERIC-PCR produced 98 bands with a range of 150-8000 bps. Overall 16 genomic profile with 30 to 86% and for a few strains, 100% similarities were produced based on Rep-PCR. Conclusion: Overall, all isolates showed polymorphic band patterns and no monochromic band was observed for the isolates. The presence of polymorphic band patterns in these techniques shows high rates of polymorphism in the genome of P. aeruginosa. Furthermore, the techniques used in this study are reliable approaches for genotyping of P. aeruginosa.
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