Isolation and identification of bacteria from water and sediment of silver carp broodstocks ponds
Subject Areas : Applied Microbiology
Mehran Avakh Keysami
1
*
,
Ali Mohammadpour
2
,
محمد رهاننده
3
,
Afshar Zoughi Shalmani
4
1 - Aquatics and Fisheries Research Department, Gilan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran.
2 - Environmental research Department, Guilan Environmental General Office of Environment, Iranian Environment Organisation, Rasht, Iran.
3 - Environmental research Department, Guilan Environmental General Office of Environment, Iranian Environment Organisation, Rasht, Iran.
4 - Aquatics and Fisheries Research Department, Gilan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran.
Keywords: silver carp, bacterial flora, Hypophthalmichthys molitrix, biolog, micolog.,
Abstract :
In silver carp farming, infectious diseases caused by bacteria are one of the important causes of mortality that occurs following changes in the quality of the culture water. The aim of this study was to isolate and identify the bacterial flora of water and sediment in silver carp farming ponds in the Sangar of Rasht. Four farms with Hypophthalmichthys molitrix broodstocks (76.5 ±0.99 kg) were considered as sampling sites. In order to isolate and identify the bacterial flora of water and sediment, the samples prepared individually were used for preliminary and differential microbial culture. The grown samples were re-cultivated, purified and their properties were identified to the species level based. No significant differences were observed in the study of the physicochemical variables of the water and the bacterial count of the sampled farms. Totally 11 genera were identified from the water and sediment of the breeding ponds, the most abundant of which were the genera Bacillus, Aeromonas and Pseudomonas. According to the results of this study, the difference in the density of gram-negative and gram-positive bacteria isolated from the samples was not significant. Although a large number of bacteria isolated in this study are known to be pathogenic, they are not primary pathogens and are considered part of the natural microflora in the body of aquatic animals, in seawater, estuaries and freshwater environments. Of course, in conditions of stress and degradation of the immune system of aquatic animals, they can become pathogenic.
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