Comparison and optimization of antioxidant compounds produced by autochthonous and allochthonous Aspergillus strains

Subject Areas : Applied Microbiology

Fatemeh Moshki ¹ (M.Sc. student, Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran)
Nafiseh Sadat Naghavi ² (Assistant Professor, Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.)
Masoud Fouladgar ³ (Associate Professor, Department of Basic Sciences, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.)

Received: 2019-04-15 Accepted : 2019-04-23 Published : 2019-11-22

Keywords: Optimization, Aspergillus, Antioxidant, Screening,

Abstract :

Background & Objectives: Antioxidants reduce the risk of cardiovascular diseases, and stroke and prevent the development of cancer by neutralizing free radicals. The present study aimed to produce and optimize the production of antioxidant compounds by Aspergillus strains.Materials & Methods: Fungi samples were collected from different regions of Isfahan province or obtained from the Persian Type Culture Collection. Purified fungi samples were inoculated to the Czapek medium to produce antioxidant compounds. Screening and comparison of antioxidant properties of the selected strains were carried out using four assays including free DPPH (Diphenyl pycryle-Hydrazyl) radical reduction, iron radical recovery, and total phenol content and flavonoid content assays. Fungi antioxidant activity was optimized in terms of pH, temperature, and type of carbon and nitrogen sources by single factor and detailed factorial (Taguchi) designed experiments.Results: The highest antioxidant activity was observed in Aspergillus niger isolated from the soil of the Iron melting factory and the highest amount of flavonoid content was shown by Aspergillus fumigatus isolated from greenhouse soil. DPPH radical reduction rate by Aspergillus niger was 89.9% in the optimum condition (pH 6, the temperature of 25°C, sucrose as carbon source and potassium nitrate as nitrogen source). Optimization by Taguchi designed experiments resulted in a 10-15 percent increase in antioxidant compounds production.Conclusion: Autochthonous isolated fungi had high potential to produce antioxidant compounds and can be proposed to related industries.

References:

 1. Alaei A, Hamedi J, Panah Mohammadi F, Motaed Mohammadi S, Rezayat M. Evaluation of
antioxidant activity and thyroid toxicology of native actinomites of Iran. J Cell Biol Mol. 2014;
5(19): 9-19 [In Persian].
2. Gupta C, Prakash D, Gupta S. Natural useful therapeutic products from microbes. J Microbiol
Exper. 2014; 1(1): 1-9.
3. Shariat Zadeh M, Dezfoulian A, Fanny A, Maleki rad A. Free radicals and antioxidants. Tehran.
Aeez publications; 2007 [In Persian].
4. Malpure P, Shah A, Juvekar A. Antioxidant and anti-inflammatory activity of extract obtained
from Aspergillus candidus MTCC 2202 broth filtrate. Ind J Exp Biol. 2006; 44(6): 468- 473.
5. Chandra P, Arora D. Assay of antioxidant potential of two Aspergillus isolates by different
methods under various physio-chemical conditions. Braz J Microbiol. 2010; 41(3): 765-777.
دنیای میکروبها، سال دوازدهم شماره سوم پاییز .1389مقایسه و بهینه سازی تولید ترکیبات آنتی اکسیدان سویه های بومی و غیر بومی قارچ آسپرژیلوس. فاطمه مشکی و همکاران
249
6. Sun T, Powers J, Tang J. Loss of rutin and antioxidant activity of Asparagus juice caused by a
pectolytic enzyme preparation from Aspergillus niger. Food Chem. 2007; 115(1): 173-178.
7. Yadav M, Yadav A, Yadav J. In vitro antioxidant activity and total phenolic content of
endophytic fungi isolated from Eugenia jambolana Lam. Asian Pac J Trop Med. 2014; 7(1):
256- 261.
8. Hosseini S, qrachorelo M, Tarzi ghiasi B, ghavami M. A review on the methods of
determination of antioxidant capacity. Food Sci Nutr. 2014; 4(11): 84-110 [In Persian].
9. Perrone G, Susca A, Cozzi G, Ehrlich K, Varga J. Frisvad C, Meijer M, Noonim P,
Mahakarnchanakul W, Samson R. Biodiversity of Aspergillus species in some important
agricultural products. Stud Mycol. 2007; 59(7): 53-66.
10. Blainski A, Lopes G, Palazzo J. Application and analysis of the folin ciocalteu method for the
determination of the total phenolic content from Limonium brasiliense. Molecules. 2013; 18 (6):
6852-6865.
11. Mariken J, Haenen , Hans J, Voss P , Bast A. Antioxidant capacity of reaction products limits
the applicability of the trolox equivalent antioxidant capacity (TEAC) Assay. Food Chem
Toxicol. 2004; 42(1): 45-49.
12. Chen Q, Chen J, Du H, Li Q, Chen J, Zhang G, Liu H, Wang H. Structural characterization and
antioxidant activities of polysaccharides extracted from the pulp of Elaeagnus angustifolia. Int J
Mol Sci. 2014; 15(7): 11446-11455.
13. Akhavan sepahi A, farahani S. 2014. Soil microbiology. Tehran. Etminan publishing; 1972.
[In Persian].
14. Cerecetto H, Ferrandiz M. Antioxidant activity of Aspergillus fumigatus. ISRN Pharmacol.
2011; 2011(2011): 67-78.
15. Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other
oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods Enzymol.
1999; 299(1): 152-178.
16. Hamed S, Mahamoud M, Sayed E, Hamed S. Scavenging activity of secondary metabolites
extracted from food borne fungi. J Chem Pharm. 2016; 8(3): 600-604.
17. Beketov E, Pakhomov V, Nesterova O. Improved method of flavonoid extraction from bird
cherry fruits. Pharm Chem J. 2005; 39(6): 316-318.
18. Chandra P, Arora D. Antioxidant potential of fungal isolates assayed through various
procedures, screening of functional compounds and their purification from Aspergillus terreus.

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 1. Alaei A, Hamedi J, Panah Mohammadi F, Motaed Mohammadi S, Rezayat M. Evaluation of
antioxidant activity and thyroid toxicology of native actinomites of Iran. J Cell Biol Mol. 2014;
5(19): 9-19 [In Persian].
2. Gupta C, Prakash D, Gupta S. Natural useful therapeutic products from microbes. J Microbiol
Exper. 2014; 1(1): 1-9.
3. Shariat Zadeh M, Dezfoulian A, Fanny A, Maleki rad A. Free radicals and antioxidants. Tehran.
Aeez publications; 2007 [In Persian].
4. Malpure P, Shah A, Juvekar A. Antioxidant and anti-inflammatory activity of extract obtained
from Aspergillus candidus MTCC 2202 broth filtrate. Ind J Exp Biol. 2006; 44(6): 468- 473.
5. Chandra P, Arora D. Assay of antioxidant potential of two Aspergillus isolates by different
methods under various physio-chemical conditions. Braz J Microbiol. 2010; 41(3): 765-777.
دنیای میکروبها، سال دوازدهم شماره سوم پاییز .1389مقایسه و بهینه سازی تولید ترکیبات آنتی اکسیدان سویه های بومی و غیر بومی قارچ آسپرژیلوس. فاطمه مشکی و همکاران
249
6. Sun T, Powers J, Tang J. Loss of rutin and antioxidant activity of Asparagus juice caused by a
pectolytic enzyme preparation from Aspergillus niger. Food Chem. 2007; 115(1): 173-178.
7. Yadav M, Yadav A, Yadav J. In vitro antioxidant activity and total phenolic content of
endophytic fungi isolated from Eugenia jambolana Lam. Asian Pac J Trop Med. 2014; 7(1):
256- 261.
8. Hosseini S, qrachorelo M, Tarzi ghiasi B, ghavami M. A review on the methods of
determination of antioxidant capacity. Food Sci Nutr. 2014; 4(11): 84-110 [In Persian].
9. Perrone G, Susca A, Cozzi G, Ehrlich K, Varga J. Frisvad C, Meijer M, Noonim P,
Mahakarnchanakul W, Samson R. Biodiversity of Aspergillus species in some important
agricultural products. Stud Mycol. 2007; 59(7): 53-66.
10. Blainski A, Lopes G, Palazzo J. Application and analysis of the folin ciocalteu method for the
determination of the total phenolic content from Limonium brasiliense. Molecules. 2013; 18 (6):
6852-6865.
11. Mariken J, Haenen , Hans J, Voss P , Bast A. Antioxidant capacity of reaction products limits
the applicability of the trolox equivalent antioxidant capacity (TEAC) Assay. Food Chem
Toxicol. 2004; 42(1): 45-49.
12. Chen Q, Chen J, Du H, Li Q, Chen J, Zhang G, Liu H, Wang H. Structural characterization and
antioxidant activities of polysaccharides extracted from the pulp of Elaeagnus angustifolia. Int J
Mol Sci. 2014; 15(7): 11446-11455.
13. Akhavan sepahi A, farahani S. 2014. Soil microbiology. Tehran. Etminan publishing; 1972.
[In Persian].
14. Cerecetto H, Ferrandiz M. Antioxidant activity of Aspergillus fumigatus. ISRN Pharmacol.
2011; 2011(2011): 67-78.
15. Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other
oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods Enzymol.
1999; 299(1): 152-178.
16. Hamed S, Mahamoud M, Sayed E, Hamed S. Scavenging activity of secondary metabolites
extracted from food borne fungi. J Chem Pharm. 2016; 8(3): 600-604.
17. Beketov E, Pakhomov V, Nesterova O. Improved method of flavonoid extraction from bird
cherry fruits. Pharm Chem J. 2005; 39(6): 316-318.
18. Chandra P, Arora D. Antioxidant potential of fungal isolates assayed through various
procedures, screening of functional compounds and their purification from Aspergillus terreus.