The comparison between molecular and bacteriological detection for identification of abortion agents caused by Brucella and Salmonella in sheep in Shahrekord town

Subject Areas : Molecular Microbiology

Ali Sharifzadeh ¹ , Abbas Doosti ² , Mohsen Gaafarian ³

1 - Department of Microbiology, Faculty of Veterinary Medicine , Islamic Azad University, Shahrekord branch, Shahrekor, Iran
2 - Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
3 - Department of Microbiology, Faculty of Veterinary Medicine , Islamic Shahrekord branch, Shahrekor, Iran

Received: 2009-06-15 Accepted : 2009-08-16 Published : 2009-07-01

Keywords: culture, Multiplex PCR, Brucella spp, abortion, Salmonella abortus ovis,

Abstract :

Background and Objective: Brucella spp and Salmonella abortus ovis are important causes of ovine abortion around the world. Both Bacteria can be serologically diagnosed, but many factors may cause false positive and negative results. Direct methods based on bacteriological isolation are usual, but they are difficult, time consuming and dangerous. Polymerase chain reaction (PCR) have been successfully described for the detection of Brucella spp. and Salmonella abortus ovis. Material and Methods: The detection of these agents in aborted ovine fetuses by multiplex PCR is described. The mPCR was applied to 38 fetal stomach contents. 5(13.1%) samples collected from ovine fetus were Brucella spp. Results: 19 (50%) samples collected were salmonella abortus ovis. 10 (26.3%) samples collected were negative and 4 (10.6%) samples collected were Brucella spp. and  Salmonella abortus ovis .in Bacteriological examination 5(13.1%)samples collected from ovine fetus were Brucella spp. 9(23.7%)samples collected were salmonella abortus ovis and 24 (63.2%) samples collected were negative. Conclusion: Simplicity and the possibility of detection of both bactera in a single tube reaction support the use of the mPCR in the routine diagnosis.