Identification and enzymatic evaluation of peptidase producing bacteria

Subject Areas : Molecular Microbiology

Mojtaba Mortazavi ^{1 *} , Batool Khani Sakhvidi ² , Masoud Torkzadeh-Mahani ³ , safa lotfi ⁴ , Mahmood Maleki ⁵ , Mehdi Rahimi ⁶

1 - Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.
2 - Department of Biodiversity, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.
3 - Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.
4 - Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.
5 - Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.
6 - Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.

Received: 2024-09-15 Accepted : 2024-12-02 Published : 2024-12-10

Keywords: Protease enzyme, srRNA 16 gene, phylogenetic tree, protease activity, enzyme-producing bacteria ,

Abstract :

Background & Objectives: Proteases, hydrolases used extensively in industries like detergents and textiles, are particularly valuable when sourced from microbes due to their resilience. This study aims to identify bacteria capable of producing proteases and to investigate their enzymatic properties. Understanding the function and efficiency of these enzymes can lead to process improvements and novel applications in various industries.

Materials & Methods: Bacterial protease-producing bacteria were isolated from water and soil samples collected in Kerman and screened on SMA based on halo diameter. The species exhibiting the highest protease activity was selected, its 16S rDNA sequenced, and identified using BLAST. Protease activity was then evaluated at different temperatures.

Results: Enzyme tests show that sample 11b has protease activity in the temperature range of 25-45°C. After bacterial DNA extraction, PCR processing, and epidermal sequencing, Staphylococcus vitellinus, Bacillus toyonensis, Citrobacter jillini, and Staphylococcus sevieri were identified.

Conclusion: This analysis introduces a new bacterial species that can be used in various industrial and clinical fields.

References:

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