Isolation, identification and investigation of the relationship between multidrug resistance (MDR) and the presence of mexC, exoS and plcH genes in P. aeruginosa clinical isolates
Subject Areas : Molecular MicrobiologyLeyli Ali Abd Alhasinat 1 , Bita Behboodian 2 , Samane Dolatabadi 3 , Elahe Vadayekheiry 4
1 - Department of biology, Science and Research Branch, Islamic Azad University Tehran, Iran
2 - Department of agriculture, kashmar Branch, Islamic Azad University, kashmar , Iran
3 - .Department of Microbiology, Neyshabur branch Islamic Azad Univercity, Neyshabur, Iran
4 -
Keywords: Pseudomonas aeruginosa, drug resistance, phospholipase C, exotoxin, mexC.,
Abstract :
Pseudomonas aeruginosa causes serious hospital acquired infections. The prevalence of multidrug-resistant strains reduces treatment options and significantly increases complication rates. Due to the importance of this topic, knowledge of pathogenic mechanisms and antibiotic resistance seems essential. The aim of this study is to evaluate drug resistance factors in Pseudomonas aeruginosa through the expression of mexC, exoS and plcH. First, 150 clinical samples of hospitalized patients were collected. P. aeruginosa isolates were identified by biochemical tests. Antibiotic sensitivity phenotype of P. aeruginosa isolates was done by disk diffusion method. PCR technique was used to check the presence of mexC, exoS and plcH genes in resistant isolates. The results showed that 66% of the samples were infected with P. aeruginosa, and the highest frequency of contamination was related to blood samples. According to the obtained results, the frequency of resistance to cefixime antibiotic was the highest and 86%. The lowest percentage of resistance to imipenem was 42%, 60% of isolates had MDR phenotype. PCR analysis showed that 90% of tested resistant isolates carry the plcH gene and 75% have exoS. Due to the increase of resistance to antibiotics in hospital acquired infections, including Pseudomonas aeruginosa, it is necessary to take immediate measures for timely identification and definitive treatment with appropriate antibiotics.
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