The Effect of Ag, Cu, and ZnO Nanoparticles on TNF-α Expression in Staphylococcus aureus-Infected Balb/C Mice
Subject Areas : Molecular Microbiology
Yegane Gholami
1
,
Behnaz Esfandiari
2
*
,
Javad Arasteh
3
1 - Department of Biology, CTC., Islamic Azad University, Tehran, Iran.
2 - Department of Biology, ISIC, Islamic Azad University, Islamshahr, Iran.
3 - Department of Biology, CTC., Islamic Azad University, Tehran, Iran.
Keywords: Staphylococcus aureus, MRSA, TNF-α, Nanoparticles, MIC, MBC.,
Abstract :
Background and Objective: Antibiotic resistance, especially in methicillin-resistant Staphylococcus aureus (MRSA), is a global challenge. Nanoparticles (NPs) such as silver (Ag), copper (Cu), and zinc oxide (ZnO) have shown antibacterial potential. This study aimed to evaluate the effectiveness of these nanoparticles against MRSA and their impact on TNF-α gene expression in Balb/c mice.
Materials and Methods: Sixty-three mice were divided into nine groups of seven. Groups 5 to 9 were infected with MRSA and then treated with Ag (15.625 mg/L), Cu (62.5 mg/L), ZnO nanoparticles, vancomycin, or saline solution. TNF-α expression in the spleen was measured by Real-Time PCR on days 1 and 5. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the nanoparticles were also determined.
Results: Ag and Cu nanoparticles showed inhibitory and bactericidal effects, while ZnO was ineffective. On day 5, TNF-α expression significantly increased in the vancomycin group, followed by Ag, Cu, and ZnO groups. On day 1, only vancomycin, Ag, and Cu nanoparticles increased TNF-α expression.
Conclusion: These findings indicate that Ag NPs, through modulation of TNF-α expression in Balb/c mice, may serve as promising antibacterial agents warranting further translational studies.
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