The Application of Taguchi Method in the Optimization of Dispersive Liquid – Liquid Microextraction of Menthe piperita Floral Water

Subject Areas : Microbiology

فرشته Nematolahi ¹ , شهلا Mozafari ² , سام Saremi ³

1 - استادیار گروه شیمی، واحد تهران شرق، دانشگاه آزاد اسلامی ، تهران، ایران
2 - استادیار گروه شیمی، دانشگاه پیام نور، تهران، ایران
3 - دانشجوی کارشناسی ارشد گروه شیمی، دانشگاه پیام نور، تهران، ایران

Received: 2013-08-25 Accepted : 2013-12-06 Published : 2015-03-21

Keywords: Floral Water, Liquid -Liquid Micro Extractio, Menthe piperit, Taguchi Method,

Abstract :

Introduction: Microextraction is regarded as an extraction technique where the volume of theextracting phase is much smaller than the sample. Therefore extraction does not take placecompletely and thus a fraction of analytes is transferred in to the extracting phase. However insome cases after the extraction the concentration of extractable correlates with the initialconcentration. Therefore the aim of this research work is the optimization of liquid –liquidmicro extraction of Menthe piperita floral water.Materials and Methods: Floral water of Menthe piperita was obtained from the localmarket. Ethanol and chloroform were used as extracting solvents and the obtained chemicalcompounds were applied onto a GC- MS. Taguchi method was applied to determine theoptimum extraction of active compounds in the sample.Results: The application of Taguchi method indicated that ethanol and chloroform tetrachloride at the concentrations of 500 and 60 showed the highest microextraction raterespectively.Conclusion: 93% of the chemical compounds present in the sample were identified using theleast quantity and volume of the extracting solvent.