Optimization of Various Conditions (Temperature, Light Intensity Change, Culture Methods Batch, Fed Batch and kind of Carbon Source) for Phycocyanin Maximum Production by the Microalgae Spirulina Platensis(Arthrospira

Subject Areas : Microbiology

Keramat  Rezaee ¹ (استاد گروه علوم و مهندسی صنایع غذایی، دانشگاه تهران، تهران، ایران)
Maryam  kalantari ² (دانش آموخته کارشناسی ارشد گروه علوم و صنایع غذایی، واحد ورامین- پیشوا، باشگاه پژوهشگران جوان، دانشگاه آزاد اسلامی، ورامین، ایران)
Mahnaz  Hashemi ravan ³ (استادیار گروه علوم و صنایع غذایی، واحد ورامین- پیشوا، دانشگاه آزاد اسلامی، ورامین، ایران)
Mohamad Taghi  Golmakani ⁴ (استادیار گروه علوم و صنایع غذایی، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ایران)
Nasrin  Faragi ⁵ (دانش آموخته کارشناسی ارشد گروه علوم و صنایع غذایی، دانشگاه ارومیه، ارومیه، ایران)
Delnia  Faragi ⁶ (دانش آموخته کارشناسی ارشد گروه علوم و صنایع غذایی، واحد ورامین- پیشوا، دانشگاه آزاد اسلامی، ورامین، ایران)

Received: 2014-12-24 Accepted : 2014-12-24 Published : 2014-12-22

Keywords: Batch, Culture Methods, Fed Batch, Phycocyanin, Spirulina,

Abstract :

Introduction: Nowadays the application of natural dyes in food and pharmaceutical products are desirable and might be important. Phycociyanin is a natural pigment with powerful antioxidant activity and is extracted from the Spirulina algae. Due to the possible toxic effects of some synthetic colors and antioxidants the use of natural additives in food and pharmaceutical product are perfected. Materials and Methods: In this research, phycocyanin is produced at 30 and 35 , using glucose, ethanol and acetic acid as carbon source in both batch and semi continues methods with variable light intensitives of 2 and 3.5 Klx for maximum production. The experiments were carried out according to Full Factorial method using fixed and variable conditions on the samples. Results: The results show that in batch and semi continues methods with 1.0 mlL-1 carbon source and 2.0 Klx light intensity by increasing the temperature from 30 to 35 , the production of plycociyanin in glucose as compared to the other two carbon sources have rapidly declined. The results also indicated that by using a concentration of 1 mlL-1 carbon source and light intensity of 3.5 Klx at 30 , there were not significant differences between the batch and semi continuous methods. Conclusion: It was concluded that the amount of production by using glucose was maximum and also using ethanol showed good yields with minimal differences.